Functional development of fetal suprachiasmatic nucleus grafts in suprachiasmatic nucleus-lesioned rats

Griffioen, Henriëtte A.; Duindam, H.; Woude, T.P. van der; Rietveld, W.J.; Boer, G.J.

doi:10.1016/0361-9230(93)90021-3

Cited by 25 publications

(25 citation statements)

References 29 publications

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“…Circadian rhythms in SCN-AVP are observed both in vivo and in vitro, at the level of mRNA transcription [29], mRNA quantities [10], peptide content [25,26], and peptide release [30,42,46]. Although an output role of AVP has been hypothesized [22,39], the peptide is not a prerequisite for restoring circadian locomotor rhythms by fetal grafts in SCN-lesioned rats and hamsters [3,18,31] and in SCN-intact old rats [32]. Brattleboro rats lacking AVP expression are behaviorally rhythmic, albeit with a decreased amplitude [38].…”

Section: Introductionmentioning

confidence: 99%

Circadian dynamics of vasopressin in mouse selection lines: Translation and release in the SCN

et al. 2005

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Section: Introductionmentioning

confidence: 99%

Circadian dynamics of vasopressin in mouse selection lines: Translation and release in the SCN

et al. 2005

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“…However, the area of the SCN-like structure is commonly virtually devoid of SOM staining (DeCoursey and Buggy, 1989) and SOM-positive neurons are seen elsewhere in the surviving solid piece graft Griffioen et al, 1993). This may tentatively be interpreted as partially aberrant development of the grafted foetal SCN; the SOMergic SCN cells develop autonomically.…”

Section: Scn In Situ Vs Graftedmentioning

confidence: 92%

“…A small piece of the anterio-ventral hypothalamus obtained from foetuses and placed in the IIIrd ventricle of SCN-lesioned arhythmic hamsters and rats restored circadian rhythmicity (Kawamura and Nihonmatsu, 1983;Sawaki et al, 1984;Drucker-Colin et al, 1984;Lehman et al, 1987;DeCoursey and Buggy, 1989;Griffioen et al, 1993). The restored rhythm had moreover the genotypically determined circadian period length of the donor (Ralph et al, 1990).…”

Section: Neurotransplantation Of the Scnmentioning

confidence: 92%

“…In rat studies, VP-and VIPergic SCN-like conglomerations are clearly visible in transplants surviving one month or longer (Wiegand and Gash, 1988;Boer and Griffioen, 1990;Aguilar-Roblero et al, 1992;Griffioen et al, 1993). In hamster studies the VPergic cells are stained much more faintly than the VIPergic neurons (Lehman et al, 1987;DeCoursey and Buggy, 1989).…”

Section: Scn In Situ Vs Graftedmentioning

confidence: 93%

“…In hamster only locomotor activity has been tested in the various studies (Lehman et al, 1987;DeCoursey and Buggy, 1989;Ralph et al, 1990), and in this model even the use of dispersed cell suspension grafts with poor SCN cell survival restored rhythmicity . In rat, recovery is usually based on drinking data (Drucker-Colin et al, 1984;Sawaki et al, 1984;Boer et al, 1990;Griffioen et al, 1993) but locomotor and eating behavior revealed renewed circadian rhythms as well (Saitoh et al, 1990;Boer et al, 1993).…”

Section: Scn In Situ Vs Graftedmentioning

confidence: 99%

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Neurotransplantation of the Biological Clock in Mammals, Importance and Perspectives

Boer¹

1997

Biological Rhythm Research

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The tool of neurotransplantation has been successfully introduced in the chronobiology of mammals. Grafting of the foetal suprachiasmatic nucleus (SCN) in the IIIrd ventricle of the brain of SCNlesioned arhythmic rodents restored free-running circadian activity patterns. This ultimately proves the SCN to be the central circadian pacemaker system. However, recovery is not seen in all animals with a surviving SCN implant and the rhythm is usually not as robust as seen for the intact system. Moreover, the grafted foetal SCN has a partially deviant development, whereas the structure-function relationship after restoration of circadian rhythm was reported to differ in the various studies. This has led to two possible mechanisms of graft action: the one a circadian humoral signal diffusing into the SCN-lesioned host brain, and the other a neuritic afferent outgrowth into the brain. There is, moreover, doubt about the integration of the 'new' SCN in terms of afferent input. Given the fact that the in situ SCN has an extensive efferent and afferent system in the intact brain, the SCN grafting experiments seem to indicate that only limited aspects of the SCN can drive circadian physiological rhythms. However, on the basis of current knowledge on grafting results the present paper recommends performing more sophisticated SCN grafting experiments to contribute to the knowledge on the SCN clock system.

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Organization of neural inputs to the suprachiasmatic nucleus in the rat

1997

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The circadian timing of the suprachiasmatic nucleus (SCN) is modulated by its neural inputs. In the present study, we examine the organization of the neural inputs to the rat SCN using both retrograde and anterograde tracing methods. After Fluoro-Gold injections into the SCN, retrogradely labeled neurons are present in a number of brain areas, including the infralimbic cortex, the lateral septum, the medial preoptic area, the subfornical organ, the paraventricular thalamus, the subparaventricular zone, the ventromedial hypothalamic nucleus, the posterior hypothalamic area, the intergeniculate leaflet, the olivary pretectal nucleus, the ventral subiculum, and the median raphe nuclei. In the anterograde tracing experiments, we observe three patterns of afferent termination within the SCN that correspond to the photic/raphe, limbic/hypothalamic, and thalamic inputs. The median raphe projection to the SCN terminates densely within the ventral subdivision and sparsely within the dorsal subdivision. Similarly, areas that receive photic input, such as the retina, the intergeniculate leaflet, and the pretectal area, densely innervate the ventral SCN but provide only minor innervation of the dorsal SCN. A complementary pattern of axonal labeling, with labeled fibers concentrated in the dorsal SCN, is observed after anterograde tracer injections into the hypothalamus and into limbic areas, such as the ventral subiculum and infralimbic cortex. A third, less common pattern of labeling, exemplified by the paraventricular thalamic afferents, consists of diffuse axonal labeling throughout the SCN. Our results show that the SCN afferent connections are topographically organized. These hodological differences may reflect a functional heterogeneity within the SCN.

show abstract

Functional development of fetal suprachiasmatic nucleus grafts in suprachiasmatic nucleus-lesioned rats

Cited by 25 publications

References 29 publications

Circadian dynamics of vasopressin in mouse selection lines: Translation and release in the SCN

Circadian dynamics of vasopressin in mouse selection lines: Translation and release in the SCN

Neurotransplantation of the Biological Clock in Mammals, Importance and Perspectives

Organization of neural inputs to the suprachiasmatic nucleus in the rat

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