Functional divergence and intron variability during evolution of angiosperm TERMINAL FLOWER1 (TFL1) genes

Gao, Jian; Huang, Bing; Wan, Yu; Chang, JenYu; Li, Jun Qing; Liao, Pei Chun

doi:10.1038/s41598-017-13645-0

Cited by 13 publications

(11 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our results also recover a eudicot specific duplication resulting in the EudiCEN-like and EudiTFL1-like copies (Figure 4). All monocot TFL1-like homologs are clustered in the MonTFL1-like clade (Figure 4) consistent to what has been previously reported (Liu et al, 2016;Gao et al, 2017).…”

Section: Tfl1-like Genes Are Completely Lacking or Found In Low Numbesupporting

confidence: 89%

Evolution and Expression of Reproductive Transition Regulatory Genes FT/TFL1 With Emphasis in Selected Neotropical Orchids

et al. 2020

View full text Add to dashboard Cite

Flowering is a rigorously timed and morphologically complex shift in plant development. This change depends on endogenous as well as environmental factors. FLOWERING LOCUS T (FT) integrates several cues from different pathways acting as a flowering promoter. Contrary to the role of FT, its paralog TERMINAL FLOWER 1 (TFL1) delays floral transition. Although FT/TFL1 homologs have been studied in model eudicots and monocots, scarce studies are available in non-model monocots like the Orchidaceae. Orchids are very diverse and their floral complexity is translated into a unique aesthetic display, which appeals the ornamental plant market. Nonetheless, orchid trade faces huge limitations due to their long vegetative phase and intractable indoor flowering seasons. Little is known about the genetic basis that control reproductive transition in orchids and, consequently, manipulating their flowering time remains a challenge. In order to contribute to the understanding of the genetic bases that control flowering in orchids we present here the first broad-scale analysis of FT/TFL1-like genes in monocots with an expanded sampling in Orchidaceae. We also compare expression patterns in three selected species and propose hypotheses on the putative role of these genes in their reproductive transition. Our findings show that FT-like genes are by far more diversified than TFL1-like genes in monocots with six subclades in the former and only one in the latter. Within MonFT1, the comparative protein sequences of MonFT1A and MonFT1B suggest that they could have recruited functional roles in delaying flowering, a role typically assigned to TFL1-like proteins. On the other hand, MonFT2 proteins have retained their canonical motifs and roles in promoting flowering transition. This is also shown by their increased expression levels from the shoot apical meristem (SAM) and leaves to inflorescence meristems (IM) and floral buds (FBs). Finally, TFL1-like genes are retained as single copy and often times are lost. Their loss could be linked to the parallel recruitment of MonFT1A and MonFT1B homologs in delaying flowering and maintaining indeterminacy of the inflorescence meristem. These hypotheses lay the foundation for future functional validation in emerging model orchid species and comparative analyses in orchids with high horticultural potential in the market.

show abstract

Section: Tfl1-like Genes Are Completely Lacking or Found In Low Numbesupporting

confidence: 89%

Evolution and Expression of Reproductive Transition Regulatory Genes FT/TFL1 With Emphasis in Selected Neotropical Orchids

et al. 2020

View full text Add to dashboard Cite

show abstract

“…FT and TFL1 played important roles in flowering induction (Gao et al, 2017;Putterill & Varkonyi-Gasic, 2016). The expressions of PbFT and TFL1 homologues (gene23124 and gene16540) in pear buds showed the opposite expression patterns before and in the critical period of the flower bud differentiation.…”

Section: Expression and Function Of Pbftmentioning

confidence: 98%

Genome-wide identification of the PEBP genes in pears and the putative role of PbFT in flower bud differentiation

Zhao¹,

Wei²,

Pang³

et al. 2020

PeerJ

View full text Add to dashboard Cite

Although Phosphatidylethanolamine-binding protein (PEBP) genes have been identified in several plants, little is known about PEBP genes in pears. In this study, a total of 24 PEBP genes were identified, in which 10, 5 and 9 were from Pyrus bretschneideri genome, Pyrus communis genome and Pyrus betuleafolia genome, respectively. Subsequently, gene structure, phylogenetic relationship, chromosomal localization, promoter regions, collinearity and expression were determined with these PEBP genes. It was found that only PbFT from PEBP genes of P. bretschneideri was relatively highly expressed in leaves during flower bud differentiation. Whereas, expression patterns of TFL1 homologues, gene23124 and gene16540, were different from PbFT in buds. The expression pattern and the treatment of reduction day-length indicated that the expression of PbFT in leaves were regulated by day-length and circadian clock. Additionally, the phenotype of transgenic Arabidopsis suggested that PbFT played a role in not only promoting flower bud differentiation, but also regulating the balance between vegetative and reproductive growth. These results may provide important information for further understanding of the evolution and function of PEBP genes in pears.

show abstract

“…The TFL1 ancestor underwent two separate duplication events in the common ancestor of angiosperms, which created three lineages corresponding to TFL1, BROTHER OF FT AND TFL1 (BFT), and the CENTRORADIALIS homolog (ATC or CEN) [10]. The TFL1/BFT/CEN-like genes control inflorescence meristem identity and delay transition to the reproductive phase [11] and are involved in growth and dormancy cycles [12], and seasonality of flowering in perennials [13,14]. Furthermore, the proteins from the TFL1/CEN/BFT-like subfamily can act as anti-florigen, a transmissible flowering repressor [15].…”

Section: Introductionmentioning

confidence: 99%

Phylogenomic Analysis of the PEBP Gene Family from Kalanchoë

et al. 2019

View full text Add to dashboard Cite

The PEBP family comprises proteins that function as key regulators of flowering time throughout the plant kingdom and they also regulate growth and plant architecture. Within the PEBP protein family, three subfamilies can be distinguished in angiosperms: MOTHER OF FT AND TFL1-like (MFT), FLOWERING LOCUS T-like (FT-like), and TERMINAL FLOWER1-like (TFL1-like). Taking advantage of the genome sequences available from K. fedtschenkoi and K. laxiflora, we performed computational analysis to identify the members of the PEBP gene family in these species. The analyses revealed the existence of 11 PEBP genes in K. fedtschenkoi and 18 in K. laxiflora, which are clustered in two clades: FT-like and TFL1-like. The PEBP genes had conserved gene structure and the proteins had highly conserved amino acid sequences in the positions crucial for the protein functions. The analysis of Ka/Ks ratio revealed that most recently duplicated genes are under positive selection. Despite being an economically important genus, the genetics underlying the regulation of flowering in Kalanchoë is poorly understood. The results of this study may provide a new insight into the molecular control of flowering that will allow further studies on flowering control in Kalanchoë.

show abstract

Functional divergence and intron variability during evolution of angiosperm TERMINAL FLOWER1 (TFL1) genes

Cited by 13 publications

References 68 publications

Evolution and Expression of Reproductive Transition Regulatory Genes FT/TFL1 With Emphasis in Selected Neotropical Orchids

Evolution and Expression of Reproductive Transition Regulatory Genes FT/TFL1 With Emphasis in Selected Neotropical Orchids

Genome-wide identification of the PEBP genes in pears and the putative role of PbFT in flower bud differentiation

Phylogenomic Analysis of the PEBP Gene Family from Kalanchoë

Contact Info

Product

Resources

About