Functional divergence of RrGL3 and RrEGL3 from Rosa roxburghii in mediating trichome development

Yan, Huiqing; Wu, Zongmin; Liu, Yanjing; Weng, Qingbei; Yi, Yin; Huang, Xiaolong

doi:10.1007/s11240-021-02125-z

Cited by 9 publications

(11 citation statements)

References 44 publications

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“…Previous studies have been mainly focused on investigating the morphological structure and chemical composition of R. roxburghii [ 28 , 29 , 30 ], the developmental stage of R. roxburghii [ 31 ], and L-ascorbic acid synthesis of R. roxburghii [ 1 ], while only a few reported on differences among R. roxburghii genotypes [ 32 ]. In this study, we focused on the fruit quality and metabolic differences among R. roxburghii genotypes.…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Fruit Metabolome Using Widely Targeted Metabolomics Reveals Nutritional Characteristics of Different Rosa roxburghii Genotypes

Jiang

Lü

Rao

et al. 2022

Foods

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The fruits of Rosa roxburghii (R. roxburghii) Tratt., which are rich in bioactive compounds, provide numerous health benefits, yet the overall metabolism of R. roxburghii fruits and the metabolic profiles among different genotypes of R. roxburghii fruits are not fully understood. In the research, we used ultra-performance liquid chromatography/tandem mass spectrometry analysis to identify and quantify metabolites including phenolic acids, amino acids, and organic acids in six R. roxburghii genotypes; a total of 723 metabolites were identified. Comparative analysis showed some different characteristic metabolites in each genotype. Moreover, flavonoids, triterpenoids, and phenolic acids were significantly correlated with the antioxidant capacity of the fruit extract. Our results suggest that R. roxburghii fruits have rich bioactive metabolites beneficial to human health and that Rr-7 and Rr-f have more potential to be used as medicinal material or functional food than other genotypes. This research provides helpful information for developing new functional foods of R. roxburghii genotypes.

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Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Fruit Metabolome Using Widely Targeted Metabolomics Reveals Nutritional Characteristics of Different Rosa roxburghii Genotypes

Jiang

Lü

Rao

et al. 2022

Foods

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“…Various types of genes and plant hormones are involved in the development of plant epidermal structures (Shibata and Sugimoto, 2019;Wang et al, 2019c;Nunes et al, 2020). Many transcription factors play important roles in plant epidermal cell development, such as MYB transcription factors (Wang et al, 2019b;Zhang et al, 2019;Xu et al, 2021) (Wang et al, 2019c;Yan et al, 2021), WD40 proteins (Yuan et al, 2019b), HD-ZIP transcription factors (Zhang et al, 2021), and zinc finger proteins (Chang et al, 2018;Yan et al, 2021). C2H2 zinc finger proteins, which play important roles in a variety of biological processes, comprise the largest transcription family in plants and are widely distributed in many species (Sakamoto et al, 2004;Han et al, 2020b;Zhang et al, 2020;Yang et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Advances in the Regulation of Epidermal Cell Development by C2H2 Zinc Finger Proteins in Plants

Han

Qiao

et al. 2021

Front. Plant Sci.

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Plant epidermal cells, such as trichomes, root hairs, salt glands, and stomata, play pivotal roles in the growth, development, and environmental adaptation of terrestrial plants. Cell fate determination, differentiation, and the formation of epidermal structures represent basic developmental processes in multicellular organisms. Increasing evidence indicates that C2H2 zinc finger proteins play important roles in regulating the development of epidermal structures in plants and plant adaptation to unfavorable environments. Here, we systematically summarize the molecular mechanism underlying the roles of C2H2 zinc finger proteins in controlling epidermal cell formation in plants, with an emphasis on trichomes, root hairs, and salt glands and their roles in plant adaptation to environmental stress. In addition, we discuss the possible roles of homologous C2H2 zinc finger proteins in trichome development in non-halophytes and salt gland development in halophytes based on bioinformatic analysis. This review provides a foundation for further study of epidermal cell development and abiotic stress responses in plants.

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“…The CDS of RrTTG1 was cloned in-frame into the Xba I-digested pM999-eGFP plasmid, which contains an enhancer green fluorescent protein (eGFP) tag driven by CaMV 35S promoter. The constructed and empty vector plasmids were independently transformed into suspension protoplasts of Arabidopsis by PEG (polyethylene glycol) transformation ( Yan et al, 2021 ). The fusion protein localization was monitored in the next 24 h. The nucleus was stained with 4′,6-diamidino-2-phenylindole (DAPI; Roche, Switzerland) at 0.1 μg/mL for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…Previously, we found that RrGL1 from R. roxburghii was evolutionarily similar to GL1 and positively regulated the epidermal cell fate ( Huang et al, 2019 ). RrEGL3 had significantly higher expression than RrGL3 in R. roxburghii ( Yan et al, 2021 ). However, EGL3 in Arabidopsis had a much smaller effect than GL3 on trichome development ( Bernhardt et al, 2003 ).…”

Section: Introductionmentioning

confidence: 98%

“…It is a typical structure involved in thermoregulation and defense against multiple biotic and abiotic stresses ( Kellogg et al, 2011 ). Rosa roxburghii Tratt, a Chinese perennial Rosaceae rosebush, is well-known for its high nutritional and medicinal values, but produces prickles on the surface of stem, sepals, petioles, and fruits ( Wang D. J. et al, 2019 ; Yan et al, 2021 ). Its fruit prickles exhibit multiple morphological structures, including flagelliform, triangular, conical, and acicular glandular shapes at various stages ( Wang D. J. et al, 2019 ; Li et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

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RrTTG1 promotes fruit prickle development through an MBW complex in Rosa roxburghii

Huang

Liu

et al. 2022

Front. Plant Sci.

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Fruit prickles are widely distributed on the pericarp and exhibit polymorphic traits at different developmental stages. Although they are multicellular appendages that are well-known for helping plants defend against biotic and abiotic stresses, their origination and molecular mechanism are still less known. Here, we studied the origination and molecular mechanism of fruit prickles in Rosa roxburghii. Using morphological and histological observations, we found that the fruit prickle primordium of R. roxburghii originated from the ground meristem that underwent cell division to form flagelliform prickles, continued to enlarge, and finally lignified to form mature fruit prickles. We amplified a homolog of candidate gene TRANSPARENT TESTA GLABRA1 (TTG1) from R. roxburghii, named RrTTG1. RrTTG1 harbored four conserved WD-repeat domains and was exclusively nuclear-localized. Using qRT-PCR and in situ hybridization, we found that RrTTG1 was constitutively expressed and highly expressed during the initiation and cell expansion phases of fruit prickles. Ectopic expression analysis in Arabidopsis proved that RrTTG1 substantially enhanced the number of trichome and pigmentation production and inhibited root hair formation. Besides, RrTTG1 complemented the phenotypes of the ttg1 mutant in Arabidopsis, thus indicating that RrTTG1 played pleiotropic roles akin to AtTTG1. We demonstrated that the RrTTG1 only interacted with RrEGL3, a homolog of ENHANCER OF GLABRA3 (EGL3), via yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays. Briefly, RrTTG1 might positively regulate the initiation of fruit prickle primordium and cell enlargement by forming the RrTTG1-RrEGL3-RrGL1 complex in R. roxburghii. Therefore, our results help characterize the RrTTG1 in R. roxburghii and also elucidate the establishment of the prickles regulatory system in the Rosaceae plants.

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Functional divergence of RrGL3 and RrEGL3 from Rosa roxburghii in mediating trichome development

Cited by 9 publications

References 44 publications

Comparative Analysis of Fruit Metabolome Using Widely Targeted Metabolomics Reveals Nutritional Characteristics of Different Rosa roxburghii Genotypes

Comparative Analysis of Fruit Metabolome Using Widely Targeted Metabolomics Reveals Nutritional Characteristics of Different Rosa roxburghii Genotypes

Advances in the Regulation of Epidermal Cell Development by C2H2 Zinc Finger Proteins in Plants

RrTTG1 promotes fruit prickle development through an MBW complex in Rosa roxburghii

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