Functional Dyspepsia is Associated with cagA-positive Helicobacter pylori Strains

Loffeld, R.J.L.F.; Werdmuller, B. F. M.; Kusters, Johannes G.; Kuipers, E.J.

doi:10.1080/003655201300051072

Cited by 21 publications

(15 citation statements)

References 15 publications

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“…VIP is also one of the main inhibitory neurotransmitters acting on intestinal muscle and its level is altered during gut inflammation 25 . Chronic H. pylori infection can cause a twofold increase in the density of VIP immunoreactive nerves in the stomach of mice 26 . In this study a significant increase (twofold to threefold) in the expression of VIP in the stomach and dorsal horn of the spinal cord of mice was noted in the course of both acute and chronic H. pylori infection, and this may explain in part the generation of impaired accommodation and delayed gastric emptying associated symptoms such as belching, fullness, early satiety, nausea and vomiting.…”

Section: Discussionmentioning

confidence: 99%

“…H. pylori infection and the related gastric mucosal inflammatory response may lead to increased visceral sensitivity and dyspeptic symptom perception through the sensitization of afferent neuronal endings 2,15 . H. pylori infection and gastritis were associated with functional dyspepsia 26,27 . The resolution of gastritis after H. pylori eradication was a predictor of symptomatic improvement in patients with functional dyspepsia 10 …”

Section: Discussionmentioning

confidence: 99%

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Role of Helicobacter pylori infection on neuronal expression in the stomach and spinal cord of a murine model

Chen

Lü

et al. 2009

J of Digest Diseases

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OBJECTIVE:To investigate the effect of Helicobactor pylori (H. pylori) infection on neuronal expressions in the stomach and spinal cord of mice so as to explain dyspepsia symptoms in H. pylori infected patients.METHODS: C57BL/6 female mice were studied at 2 weeks (acute infection group) and 12 weeks (chronic infection group) after H. pylori inoculation. Histological analyses for gastric inflammation and bacterial colonization were assessed by HE staining and Warthin-Starry staining. Fos, vasoactive intestinal polypeptide (VIP) and calcitonin generelated peptide expressions (CGRP) were studied by immunohistochemistry. RESULTS:H. pylori colonization was present mainly in pyloric region, but bacterial density was similar in both infected groups. The intensity of mucosal inflammation and activity was significantly higher in two infected groups than in those in the control group. The degree of mononuclear and polymorphonuclear cell infiltration in proventricular-glandular region and gastric corpus at 12 weeks after H. pylori inoculation was higher than that at 2 weeks after inoculation. The neuronal expressions of fos, VIP, and CGRP in the stomach and spinal cord were significantly more marked in the infected groups than in the control group, but there was no significant difference between two infected groups. CONCLUSION: H. pylori infection induced differentdegrees of gastric mucosal inflammation in the murine model. Both early and chronic infection groups of mice showed enhanced neuronal expressions of fos, VIP and CGRP of stomach and spinal cord and these could form a basis for appearance of functional dyspeptic symptoms in patients with H. pylori infection.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Role of Helicobacter pylori infection on neuronal expression in the stomach and spinal cord of a murine model

Chen

Lü

et al. 2009

J of Digest Diseases

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“…So far, many attempts have been made to find serological markers of H. pylori virulence, which could be correlated with the severity of the H. pylori-associated diseases. These studies indicate that seropositivity to some H. pylori antigens could be used as serological marker for bacterial virulence (17)(18)(19)(20)28,29).…”

Section: Discussionmentioning

confidence: 99%

Serum Antibodies Positivity to 12 Helicobacter pylori Virulence Antigens in Patients with Benign or Malignant Gastroduodenal Diseases – Cross-sectional Study

et al. 2009

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Aim To investigate the association of gastric histological and endoscopic findings in patients with Helicobacter pylori (H. pylori), according to presence of seropositivity to 12 bacterial virulence antigens.Methods This is a cross-sectional single-center study of 360 consecutive outpatients referred in the period of one year to upper gastrointestinal endoscopy because of dyspeptic complaints. Patients sera were tested by Western blot method to determine the presence of serum antibodies to bacterial virulence antigens -p120 (CagA -cytotoxin-associated antigen), p95 (VacA -vacuolating cytotoxin), p67 (FSH -flagellar sheath protein), p66 (UreB -urease enzyme heavy subunit), p57 (HSP homologue -heath shock protein homologue), p54 (flagellin), p33, p30 (OMP -outer membrane protein), p29 (UreA -urease enzyme light subunit), p26, p19, and p17. Upper gastrointestinal endoscopy was performed, endoscopic diagnosis recorded, and 4 mucosal biopsy samples were obtained and assessed according to Updated Sydney protocol. ResultsThe sera of 207 patients were analyzed. Thirty patients had gastric adenocarcinoma, 126 peptic ulcers, and 51 normal finding. p120 (CagA) seropositivity was significantly more often present in patients with higher activity grade in the antrum (P = 0.025), p30 in patients with greater inflammation in the antrum (P = 0.025) and the corpus (P = 0.010), p33 in patients with greater inflammation in the corpus (P = 0.050), and p19 (OMP) in patients with lower intestinal metaplasia grades in the corpus (P = 0.025). Seroreactivity to all other bacterial proteins showed no association with the histological status of the stomach mucosa. Except for the seropositivity to protein p95 (VacA), which was more often present in patients with duodenal ulcer (P = 0.006), there was no difference in seroreactivity to other bacterial proteins and upper gastrointestinal endoscopic findings.Conclusions p120 (CagA), p33, p 30 (OMP), and p19 (OMP) seropositivity was more often present in patients with higher grades of the histological parameters of gastritis and seropositivity to protein p95 (VacA) with endoscopic presence of duodenal ulcer. Histological parameters of gastritis are more associated with bacterial virulence than endoscopic findings.

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“…There is definitely a link between the clinical presentation and the virulence status of H.pylori [9].…”

Section: Discussionmentioning

confidence: 99%

“…A serum sample was taken and frozen at minus 70 degrees Celsius. Specific IgG antibodies against the H.pylori cagA were determined as described earlier [9,11].…”

Section: Introductionmentioning

confidence: 99%

Long-Term Outcome Of Patients With Functional Dyspepsia Infected With A Cag-Positive Helicobacter Pylori Strain. A Descriptive Study

Kemps¹,

Loffeld²

2017

JGPLD

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Functional Dyspepsia is Associated with cagA-positive Helicobacter pylori Strains

Abstract: Functional dyspeptics with cagA-positive H. pylori strains have more dyspeptic symptoms and higher symptom scores than patients with cagA-negative H. pylori strains as well as H. pylori-negative functional dyspeptics.

Cited by 21 publications

References 15 publications

Role of Helicobacter pylori infection on neuronal expression in the stomach and spinal cord of a murine model

Role of Helicobacter pylori infection on neuronal expression in the stomach and spinal cord of a murine model

Serum Antibodies Positivity to 12 Helicobacter pylori Virulence Antigens in Patients with Benign or Malignant Gastroduodenal Diseases – Cross-sectional Study

Long-Term Outcome Of Patients With Functional Dyspepsia Infected With A Cag-Positive Helicobacter Pylori Strain. A Descriptive Study

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