Functional endothelial progenitor cells derived from adipose tissue show beneficial effect on cell therapy of traumatic brain injury

Xue, Shan; Zhang, Hongtian; Zhang, Peng; Luo, Jie; Chen, Zhenzhou; Jang, Xiao-dan; Xu, Ran

doi:10.1016/j.neulet.2010.02.035

Cited by 45 publications

(34 citation statements)

References 21 publications

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“…Hormonal status also modulates circulating EPCs (Bulut et al, 2007). Allogeneic infusion of EPC augments hematopoiesis and increases vascular remodeling after TBI (Kalka et al, 2000;Xue et al, 2010). EPC protects the brain against ischemic injury, promotes neurovascular repair, and improves long-term neurobehavioral outcomes after stroke in mice (Fan et al, 2010).…”

mentioning

confidence: 99%

Progesterone Increases Circulating Endothelial Progenitor Cells and Induces Neural Regeneration after Traumatic Brain Injury in Aged Rats

Wang

Kan

et al. 2012

Journal of Neurotrauma

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Vascular remodeling plays a key role in neural regeneration in the injured brain. Circulating endothelial progenitor cells (EPCs) are a mediator of the vascular remodeling process. Previous studies have found that progesterone treatment of traumatic brain injury (TBI) decreases cerebral edema and cellular apoptosis and inhibits inflammation, which in concert promote neuroprotective effects in young adult rats. However, whether progesterone treatment regulates circulating EPC level and fosters vascular remodeling after TBI have not been investigated. In this study, we hypothesize that progesterone treatment following TBI increases circulating EPC levels and promotes vascular remodeling in the injured brain in aged rats. Male Wistar 20-month-old rats were subjected to a moderate unilateral parietal cortical contusion injury and were treated with or without progesterone (n = 54/group). Progesterone was administered intraperitoneally at a dose of 16mg/kg at 1 h post-TBI and was subsequently injected subcutaneously daily for 14 days. Neurological functional tests and immnunostaining were performed. Circulating EPCs were measured by flow cytometry. Progesterone treatment significantly improved neurological outcome after TBI measured by the modified neurological severity score, Morris Water Maze and the long term potentiation in the hippocampus as well as increased the circulating EPC levels compared to TBI controls ( p < 0.05). Progesterone treatment also significantly increased CD34 and CD31 positive cell number and vessel density in the injured brain compared to TBI controls ( p < 0.05). These data indicate that progesterone treatment of TBI improves multiple neurological functional outcomes, increases the circulating EPC level, and facilitates vascular remodeling in the injured brain after TBI in aged rats.

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mentioning

confidence: 99%

Progesterone Increases Circulating Endothelial Progenitor Cells and Induces Neural Regeneration after Traumatic Brain Injury in Aged Rats

Wang

Kan

et al. 2012

Journal of Neurotrauma

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“…Expression of typical endothelial markers CD31 or vWF was verified for EPC preparations from rats (a) and rabbits (b) using flow cytometry. Immunofluorescence staining of third-passage EPC cultures from rats (c) and rabbits (d) Cell Tissue Bank EPC can be isolated from umbilical cord blood (Lin et al 2011) and from various sources in adults, including PB (Yuan et al 2013), BM (Chen et al 2013), and adipose tissue (AT) (Xue et al 2010). While BM is the primary source of EPC, deriving EPC from BM or AT requires a multistep, highly invasive procedure and yields relatively few cells.…”

Section: Discussionmentioning

confidence: 99%

Combination of granulocyte colony-stimulating factor and CXCR4 antagonist AMD3100 for effective harvest of endothelial progenitor cells from peripheral blood and in vitro formation of primitive endothelial networks

Xiang

Huang

et al. 2015

Cell Tissue Bank

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Endothelial progenitor cells (EPC) derived from the circulation may be used to enhance neovascularization. Since the combination of granulocyte colony-stimulating factor (GCSF) and CXCR4 antagonist AMD3100 efficiently mobilizes hematopoietic stem cells into peripheral circulation, it may increase the pool of endogenously circulating EPC. We tested this hypothesis by administering GCSF and AMD3100 to adult rabbits and rats, isolating mononuclear cells from peripheral blood by Ficoll density gradient centrifugation, and characterizing the blood-derived EPC based on morphology, immunophenotyping, gene expression and other functional analyses. These EPC showed clonal growth similar to that of human umbilical vein endothelial cells when cultured in complete EGM-2 medium on collagen I-precoated culture plates. The EPC exhibited a typical cobblestone-like morphology and were relatively homogeneous by the third passage. The cells expressed the typical endothelial marker CD31 based on flow cytometry and fluorescence microscopy, formed capillary-like structures when cultured in Matrigel, internalized DiI-acetylated low-density lipoprotein, bound Ulex europaeus agglutinin-1, and expressed CD31 and several other endothelial markers (VEGFR2, VE-cadherin, Tie-2, eNOS, vWF) at significantly higher levels than bone marrow-derived mesenchymal stem cells. These results suggest that the combination of GCSF and AMD3100 can efficiently release stem cells into peripheral circulation and generate EPC that show the desired morphological, immunophenotypic and functional characteristics. This minimally invasive approach may be useful for autologous cell transplantation for postnatal neovasculogenesis and tissue repair.

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“…We have shown that expression of a GABA synthesizing enzyme is facilitated by pre-exposure of MSCs to Fsk and BDNF-Ad before transplantation. Brain endothelial cells forming microvessles are derived from MSCs and release neurotrophic factors such as BDNF, and NT-3 and VEGF [28][29][30] to promote differentiation of NSCs and regeneration of neurons [31]. The transplanted MSCs may behave as endogenous brain MSCs releasing neurotrophic factors.…”

Section: Discussionmentioning

confidence: 99%

Upregulation of TrkB by forskolin facilitated survival of MSC and functional recovery of memory deficient model rats

Heo

Yoo

Han

et al. 2013

Biochemical and Biophysical Research Communications

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Functional endothelial progenitor cells derived from adipose tissue show beneficial effect on cell therapy of traumatic brain injury

Cited by 45 publications

References 21 publications

Progesterone Increases Circulating Endothelial Progenitor Cells and Induces Neural Regeneration after Traumatic Brain Injury in Aged Rats

Progesterone Increases Circulating Endothelial Progenitor Cells and Induces Neural Regeneration after Traumatic Brain Injury in Aged Rats

Combination of granulocyte colony-stimulating factor and CXCR4 antagonist AMD3100 for effective harvest of endothelial progenitor cells from peripheral blood and in vitro formation of primitive endothelial networks

Upregulation of TrkB by forskolin facilitated survival of MSC and functional recovery of memory deficient model rats

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