Functional evidence that the self-renewal gene NANOG regulates esophageal squamous cancer development

Deng, Li; Xiang, Xiaocong; Yang, Fei; Xiao, Dongqin; Liu, Kang; Chen, Zhu; Zhang, Ruolan; Feng, Gang

doi:10.1016/j.bbrc.2017.06.016

Cited by 16 publications

(13 citation statements)

References 29 publications

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“…First, sensitivity to cisplatin was decreased in ECA-9706 cells highly expressing Homeobox transcription factor NANOG (607937), a gene involved in self-renewal of embryonic stem cells, while the expression of ABCB1 was increased in these cells [ 164 ]. On the other hand, NANOG knockout suppressed drug resistance to 5-fluorouracil through downregulation of ABCG2 expression and caused G1 arrest by downregulation of CCND1 in ECA-109 cells [ 165 ].…”

Section: Search Results and Discussionmentioning

confidence: 99%

ABC Transporters and Their Role in the Neoadjuvant Treatment of Esophageal Cancer

Vrána

Hlaváč

Brynychová

et al. 2018

IJMS

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The prognosis of esophageal cancer (EC) is poor, despite considerable effort of both experimental scientists and clinicians. The tri-modality treatment consisting of neoadjuvant chemoradiation followed by surgery has remained the gold standard over decades, unfortunately, without significant progress in recent years. Suitable prognostic factors indicating which patients will benefit from this tri-modality treatment are missing. Some patients rapidly progress on the neoadjuvant chemoradiotherapy, which is thus useless and sometimes even harmful. At the same time, other patients achieve complete remission on neoadjuvant chemoradiotherapy and subsequent surgery may increase their risk of morbidity and mortality. The prognosis of patients ranges from excellent to extremely poor. Considering these differences, the role of drug metabolizing enzymes and transporters, among other factors, in the EC response to chemotherapy may be more important compared, for example, with pancreatic cancer where all patients progress on chemotherapy regardless of the treatment or disease stage. This review surveys published literature describing the potential role of ATP-binding cassette transporters, the genetic polymorphisms, epigenetic regulations, and phenotypic changes in the prognosis and therapy of EC. The review provides knowledge base for further research of potential predictive biomarkers that will allow the stratification of patients into defined groups for optimal therapeutic outcome.

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Section: Search Results and Discussionmentioning

confidence: 99%

ABC Transporters and Their Role in the Neoadjuvant Treatment of Esophageal Cancer

Vrána

Hlaváč

Brynychová

et al. 2018

IJMS

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“…Esophageal cancer (EC), a highly malignant carcinoma, is estimated to be the eighth most common cancer and the sixth leading cause of cancer death worldwide [24]. Currently, chemoradiotherapy has dramatically improved the curability for unresectable malignancies compared to traditional chemotherapy or radiotherapy separately, and has been widely used to cure EC, however, serious side effects are always accompanied including tumor relapse and drug resistance [25,26]. Therefore, more and more efforts should be made to find safe and efficient bioactive substances for treating cancer.…”

Section: Discussionmentioning

confidence: 99%

Effects of Different Temperatures on the Chemical Structure and Antitumor Activities of Polysaccharides from Cordyceps militaris

et al. 2018

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The effects of different extraction temperatures (4 and 80 °C) on the physicochemical properties and antitumor activity of water soluble polysaccharides (CMPs-4 and CMPs-80) from Cordyceps militaris (C. militaris) were evaluated in this study. The results of gas chromatography (GC) and high-performance gel permeation chromatography (HPGPC) showed that a higher extraction temperature could degrade the polysaccharides with 188 kDa, mainly composed of glucose, and increase the dissolution rate of polysaccharides about 308 kDa, mainly consisting of rhamnose and galactose. In addition, the CMPs displayed the same sugar ring and category of glycosidic linkage based on Fourier-transform infrared spectroscopy (FTIR) analysis, however, their invisible structural difference occurred in the specific rotation and conformational characteristics according to the results of specific optical rotation measurement and Congo red test. In vitro antitumor experiments indicated that CMPs-4 possessed stronger inhibitory effects on human esophagus cancer Eca-109 cells by inducing cell apoptosis more than CMPs-80 did. These findings demonstrated that the polysaccharides extracted with cold water (4 °C) could be applied as a novel alternative chemotherapeutic agent or dietary supplement with its underlying antitumor property.

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“…All statistical analyses were performed using SPSS 19.0 software. 39 P < 0.05 represents statistical significance.…”

Section: Methodsmentioning

confidence: 99%

<p>miR-148a Regulates the Stem Cell-Like Side Populations Distribution by Affecting the Expression of ACVR1 in Esophageal Squamous Cell Carcinoma</p>

Tan¹,

Lü²,

Cheng³

et al. 2020

OTT

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Introduction: Esophageal squamous cell carcinoma (ESCC) is a malignant tumor disease with high mortality and morbidity rates, especially for a terminal cancer. At present, the prognosis and treatment of ESCC cannot effectively control or inhibit the spread and proliferation of tumor cells. microRNAs, a class of small spliced RNAs, are essential in the regulation of tumorigenesis and tumor cell migration and proliferation. microRNAs interact with target mRNA to silence gene expression and degrade mRNA, thereby inhibiting the expression of tumor genes or impairing the expression of tumor suppressor genes. Methods: A total of 20 human ESCC samples were collected from the Affiliated Tumor Hospital of Xinjiang Medical University. Eca109 and Kyse510 cells, which are ESCC cell lines, were subjected to FACS analysis to get side population (SP) cells and non-SP cells. Cell cycle and cell proliferation were analyzed by flow cytometry. Cell migration and invasion were detected using a transwell assay. Quantitative PCR and Western blot were performed to analyze the expression levels of ABCG2, KLF4, OCT4, and ACVR1, which are related to the stemness of stem cells. The target genes of hsa-miR-148a were predicted using TargetScan (version 7.2) and verified by a dual luciferase reporter assay. A chromatin immunoprecipitation (ChIP) assay was carried out to demonstrate direct interaction between miR-148a and ACVR1. Results: The expression of miR-148a was significantly down-regulated in ESCC cells and significantly decreased in SP esophageal squamous cells when compared to the tumor cells. By analyzing the stem cell stemness of ESCC, overexpression of miR-148a decreased the expression of ABCG2, KLF4, SOX2, OCT4, and Nanog, indicating that miR-148a may regulate stem cell function. Target gene prediction and functional annotation of miR-148a suggested that miR-148a is involved in stem cell stemness of ESCC via ACVR1. Expression of the dual luciferase-labeled gene indicates that overexpression of miR-148a inhibits the expression of ACVR1, thereby affecting stem cell stemness. Conclusion: miR-148a regulates the stem cell-like side populations distribution by inhibiting the expression of ACVR1 in ESCC. miR-148a may be a promising targeted therapy for ESCC.

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Functional evidence that the self-renewal gene NANOG regulates esophageal squamous cancer development

Cited by 16 publications

References 29 publications

ABC Transporters and Their Role in the Neoadjuvant Treatment of Esophageal Cancer

ABC Transporters and Their Role in the Neoadjuvant Treatment of Esophageal Cancer

Effects of Different Temperatures on the Chemical Structure and Antitumor Activities of Polysaccharides from Cordyceps militaris

<p>miR-148a Regulates the Stem Cell-Like Side Populations Distribution by Affecting the Expression of ACVR1 in Esophageal Squamous Cell Carcinoma</p>

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