Effects of Different Temperatures on the Chemical Structure and Antitumor Activities of Polysaccharides from Cordyceps militaris

Abstract

The effects of different extraction temperatures (4 and 80 °C) on the physicochemical properties and antitumor activity of water soluble polysaccharides (CMPs-4 and CMPs-80) from Cordyceps militaris (C. militaris) were evaluated in this study. The results of gas chromatography (GC) and high-performance gel permeation chromatography (HPGPC) showed that a higher extraction temperature could degrade the polysaccharides with 188 kDa, mainly composed of glucose, and increase the dissolution rate of polysaccharides about 308 kDa, mainly consisting of rhamnose and galactose. In addition, the CMPs displayed the same sugar ring and category of glycosidic linkage based on Fourier-transform infrared spectroscopy (FTIR) analysis, however, their invisible structural difference occurred in the specific rotation and conformational characteristics according to the results of specific optical rotation measurement and Congo red test. In vitro antitumor experiments indicated that CMPs-4 possessed stronger inhibitory effects on human esophagus cancer Eca-109 cells by inducing cell apoptosis more than CMPs-80 did. These findings demonstrated that the polysaccharides extracted with cold water (4 °C) could be applied as a novel alternative chemotherapeutic agent or dietary supplement with its underlying antitumor property.

Bioactivities of crude polysaccharide extracted from fermented soybean curd residue by Cordyceps militaris

“…Many natural polysaccharides and polysaccharide-protein complexes isolated from fungi can be used as a source of therapeutic agents that are relatively non-toxic and show no significant side effects. In recent studies, polysaccharides of C. militaris have been identified to possess antitumor [10], immunomodulatory [11], anti-oxidant [12] and anti-hypolipidemic [13] effects. In this study, the SCR was fermented by C. militaris mycelium using solid fermentation.…”

“…Â 104 cells/mL for 24 h at 37 C in a 5% CO2 atmosphere. Then cells were incubated with DOX (5 lmol/L) or LPS (1 lg/mL) in the presence or absence of various concentrations of CSCPS solutions(10,20,40, 80 lg/mL) for 24 h, 48 h and 72 h. After incubation, the medium was removed, then 100 lL of phosphate buffered saline (PBS) and 10 lL of MTT labeling reagent was added to each well for 4 h incubation.…”

Cmfhp Gene Mediates Fruiting Body Development and Carotenoid Production in Cordyceps militaris

“…[1][2][3][4]. These bioactive components endow C. militaris with anticancer, antitumor, anti-inflammatory, and antioxidant activities [5][6][7]. In recent years, novel carotenoids with good water solubility and pentostatin with anticancer activity have been found in C. militaris, and these findings have increased the market demand for C. militaris every year [8,9].…”

Structural and Biochemical Characterization of a Polysaccharide Isolated From Vaccinium uliginosum L

“…Polysaccharides with triple helix conformation can form complex with Congo red that causes a red shift in the maximum absorption wavelength (𝜆 max ). [37] Figure 4B showed the changes in 𝜆 max of Congo red-polysaccharide complexes at different alkaline concentrations (0-0.5 mol L -1 ). The red shifts in 𝜆 max were not observed in all concentrations, indicating that VUP-1 did not contain polysaccharides in a triple-helical conformation.…”