Functional expression, characterization and application of the human S100A4 protein

Wang, Degang; Zhang, Jianwei; Liu, Ziquan; Chen, Yunyun; Xu, Chuanxiang; Zhang, Zhiqing; Liu, Xiaohua; Wu, Lei; Zhou, Xuesi; Meng, Xiangyan; Li, Hua; Liu, Hongtao; Jiang, Zhi‐Yun; Wang, Tianhui

doi:10.3892/mmr.2014.2745

Cited by 3 publications

(2 citation statements)

References 17 publications

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“…The SDS-PAGE procedure was performed as previously described with slight modification (13). The concentrations of the resolving and stacking gels were 15 and 5%, respectively.…”

Section: Sds-page and Western Blot Analysis Of Recombinant S100bmentioning

confidence: 99%

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Functional expression, characterization, and application of human S100B

Wu¹,

Zhou²,

Xiao³

et al. 2017

Oncology Reports

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The EF-hand calcium-binding protein S100B presents a wide range of biological activities and functions. This binding protein is involved in various human diseases, including cancer, brain trauma and ischemia, neuro-degenerative disease (Alzheimer's disease), and psychiatric disorders. In this study, we prepared human S100B protein and its monoclonal antibodies. Human S100B protein was expressed in Escherichia coli, successfully purified by diethylaminoethyl cellulose anion-exchange chromatography, and then identified by western blot analysis. Monoclonal antibodies (mAbs) were produced by the standard hybridoma method and validated by enzyme-linked immunosorbent assay and western blot analysis. The prepared human S100B protein and its mAbs demonstrated potential biological activities. The KD of one mAb is approximately 4.72x10-8 mol/l, and its cross reactivity is low with human S100A4, mouse S100A4, and human S100A1. Recombinant Soluble S100B can promote the migration and invasion of HeLa cells. The expression of S100B protein in tumor tissues can be detected effectively by using the prepared monoclonal antibodies. Increasing concentration of the anti-human S100B mAbs showed a reduced expression of the S100B protein. Subsequently, the expression of p53 increased significantly (P<0.05) in A375 cells. A significant increase in apoptosis in A375 cells was observed with increasing S100B mAb concentration. Results showed that our prepared S100B mAbs were suitable for detecting S100B expression in human tissues, furnishing promising tools for further functional investigation and clinical applications.

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“…The SDS-PAGE procedure was performed as previously described with slight modification (13). The concentrations of the resolving and stacking gels were 15 and 5%, respectively.…”

Section: Sds-page and Western Blot Analysis Of Recombinant S100bmentioning

confidence: 99%

“…Cross reactivity. To identify the cross reactivity (CR) of the recombinant S100B and its analogs, indirect ELISA was utilized as previously described (12,13). As coated antigens, recombinant S100B, recombinant rat S100A4, human S100A4, and S100A1 were all dissolved in PBS.…”

Section: Sds-page and Western Blot Analysis Of Recombinant S100bmentioning

confidence: 99%

Functional expression, characterization, and application of human S100B

Wu¹,

Zhou²,

Xiao³

et al. 2017

Oncology Reports

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Aberrant DNA Methylation in Cutaneous Squamous Cell Carcinoma

et al. 2019

Int J Dermatol Venereol

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A modular framework for the development of targeted Covid-19 blood transcript profiling panels

et al. 2020

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Background: Covid-19 morbidity and mortality are associated with a dysregulated immune response. Tools are needed to enhance existing immune profiling capabilities in affected patients. Here we aimed to develop an approach to support the design of targeted blood transcriptome panels for profiling the immune response to SARS-CoV-2 infection. Methods: We designed a pool of candidates based on a pre-existing and well-characterized repertoire of blood transcriptional modules. Available Covid-19 blood transcriptome data was also used to guide this process. Further selection steps relied on expert curation. Additionally, we developed several custom web applications to support the evaluation of candidates. Results: As a proof of principle, we designed three targeted blood transcript panels, each with a different translational connotation: immunological relevance, therapeutic development relevance and SARS biology relevance. Conclusion: Altogether the work presented here may contribute to the future expansion of immune profiling capabilities via targeted profiling of blood transcript abundance in Covid-19 patients.

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Functional expression, characterization and application of the human S100A4 protein

Cited by 3 publications

References 17 publications

Functional expression, characterization, and application of human S100B

Functional expression, characterization, and application of human S100B

Aberrant DNA Methylation in Cutaneous Squamous Cell Carcinoma

A modular framework for the development of targeted Covid-19 blood transcript profiling panels

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