Functional expression of TRPV1 in human peripheral blood basophils and its regulation in atopic dermatitis

Limberg, Maren M.; Wiebe, Daniela; Gray, Natalie; Weihrauch, Tobias; Bräuer, Anja U.; Kremer, Andreas E.; Homey, Bernhard; Raap, Ulrike

doi:10.1111/all.15802

Cited by 3 publications

(15 citation statements)

References 6 publications

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“…Since TRPV1 activation contributes to calcium flux in human basophils [20], we analyzed the functionality of TRPV1 on eosinophils by measuring transient changes of intracellular calcium levels after channel activation via different concentrations of the specific agonist capsaicin (Figure 2A,B). We used the calcium ionophore ionomycin as a positive control which induces maximum calcium transients (Figure 2A,B).…”

Section: Activation Of Trpv1 Induces Calcium Influxmentioning

confidence: 99%

“…IL-31 is an itch-mediating cytokine which is also released by eosinophils in AD [18]. Since its receptor IL-31RA/OSMR is co-expressed with the TRPV1 ion channel on dorsal root ganglia [19], and Th1 and Th2 cytokines increase TRPV1 expression in human basophils [20] and neurons [1], it is a new candidate for playing an important role in itch. TRPV1 is a non-selective homotetrameric cation channel of the TRP superfamily.…”

Section: Introductionmentioning

confidence: 99%

“…TRPV1 can be activated by noxious stimuli like vanilloids (capsaicin), high temperature, extracellular acidification [21,23,24], chemokines [25], or inflammatory molecules such as histamine [26], adenosine triphosphate (ATP) [27], Bradykinin [28], Prostaglandin [29], and nerve growth factors like NGF [30]. The activation of TRPV1 leads to depolarization and calcium influx which then regulates cell functions like nociception, production and release of cytokines, phagocytosis, and cell migration [20,[31][32][33]. This, in turn, influences inflammation, pain, and pruritus [26,34,35].…”

Section: Introductionmentioning

confidence: 99%

“…TRPV1 is known to be expressed on sensory neurons [21]. However, TRPV1 has also been identified in different non-neuronal cells like keratinocytes, epithelial cells [36][37][38][39], and immune cells such as human mast cells [40], dendritic cells [41], T cells [42], basophils [20], neutrophils [43,44], monocytes, and macrophages [45]. Although the expression of TRPV1 on eosinophils was demonstrated by Zhu et al [46], the functional role of the channel in eosinophils is not known.…”

Section: Introductionmentioning

confidence: 99%

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TRPV1 Channel in Human Eosinophils: Functional Expression and Inflammatory Modulation

Weihrauch,

Gray,

Wiebe

et al. 2024

IJMS

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The transient receptor potential vanilloid 1 (TRPV1) is a non-selective cation channel expressed on sensory neurons and immune cells. We hypothesize that TRPV1 plays a role in human eosinophil function and is modulated by inflammatory conditions. TRPV1 expression on human eosinophils was examined by qPCR, flow cytometry, and immunohistochemistry, respectively. TRPV1 functionality was analyzed by investigating calcium flux, apoptosis, modulation by cytokines and acidic pH, and CD69 externalization using flow cytometry. Activation of TRPV1 induced calcium influx and prolonged survival. Although eosinophils were not directly activated by TRPV1 agonists, activation by IL-3 or GM-CSF was mainly restricted to TRPV1-positive eosinophils. TRPV1 surface content was increased by acidic pH, IL-3, IL-31, IL-33, TSLP, TNF-α, BDNF, and NGF-β. Interestingly, TRPV1 was also expressed by eosinophils located in proximity to peripheral nerves in atopic dermatitis (AD) skin. In conclusion, eosinophils express functional TRPV1 channels which are increased by extracellular acidification and AD-related cytokines. Since eosinophils also express TRPV1 in AD skin, our results indicate an important role of TRPV1 for neuroimmune interaction mechanisms in itchy, inflammatory skin diseases, like AD.

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Section: Activation Of Trpv1 Induces Calcium Influxmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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TRPV1 Channel in Human Eosinophils: Functional Expression and Inflammatory Modulation

Weihrauch,

Gray,

Wiebe

et al. 2024

IJMS

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“…We have used this method for publishing our research on basophils in Limberg et al. (2023) and Gray et al. (2022).…”

Section: Introductionmentioning

confidence: 99%

Density Gradient Centrifugation‐Independent Purification of Human Basophils

Gray,

Wiebe,

Weihrauch

et al. 2024

Current Protocols

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Basophils represent the rarest type of granulocyte in human peripheral blood. Thus, researching basophils has historically been challenging and has often been reliant on enrichment protocols using density gradient centrifugation. This article describes a novel, fast, and cost‐effective method to purify highly viable human basophils from peripheral blood through negative immunomagnetic selection, foregoing the density centrifugation step in the Basic Protocol. The technique is easy to use and consistently produces purities >96%. Furthermore, the Support Protocols describe procedures to determine basophil yield, purity, and viability, and how to investigate functional activity of the purified basophils through flow cytometry and visualize the basophils through microscopy. © 2024 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol: Gradient centrifugation‐independent basophil isolationSupport Protocol 1: Flow cytometry staining to assess basophil yield, purity, and viabilitySupport Protocol 2: Giemsa stainingSupport Protocol 3: Calcium flux analysisSupport Protocol 4: Basophil activation test

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TRPV1 Regulates Proinflammatory Properties of M1 Macrophages in Periodontitis Via NRF2

Li,

Guo,

Zhan

et al. 2024

Inflammation

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Functional expression of TRPV1 in human peripheral blood basophils and its regulation in atopic dermatitis

Cited by 3 publications

References 6 publications

TRPV1 Channel in Human Eosinophils: Functional Expression and Inflammatory Modulation

TRPV1 Channel in Human Eosinophils: Functional Expression and Inflammatory Modulation

Density Gradient Centrifugation‐Independent Purification of Human Basophils

TRPV1 Regulates Proinflammatory Properties of M1 Macrophages in Periodontitis Via NRF2

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