Functional, genetic and chemical characterization of biosurfactants produced by plant growth-promoting<i>Pseudomonas putida</i>267

Kruijt, M.; Tran, Ha Thi Thanh; Raaijmakers, Jos M.

doi:10.1111/j.1365-2672.2009.04244.x

Cited by 107 publications

(86 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Despite the many potential roles of biosurfactants on leaves, only a few studies have examined their production in the phyllosphere (10,(12)(13)(14)(15)(16)(17). Furthermore, these studies have focused only …”

mentioning

confidence: 99%

High-Level Culturability of Epiphytic Bacteria and Frequency of Biosurfactant Producers on Leaves

Burch

Paulina

Sbodio

et al. 2016

Appl Environ Microbiol

View full text Add to dashboard Cite

To better characterize the bacterial community members capable of biosurfactant production on leaves, we distinguished culturable biosurfactant-producing bacteria from nonproducers and used community sequencing to compare the composition of these distinct cultured populations with that from DNA directly recovered from leaves. Communities on spinach, romaine, and head lettuce leaves were compared with communities from adjacent samples of soil and irrigation source water. Soil communities were poorly described by culturing, with recovery of cultured representatives from only 21% of the prevalent operational taxonomic units (OTUs) (>0.2% reads) identified. The dominant biosurfactant producers cultured from soil included bacilli and pseudomonads. In contrast, the cultured communities from leaves are highly representative of the culture-independent communities, with over 85% of the prevalent OTUs recovered. The dominant taxa of surfactant producers from leaves were pseudomonads as well as members of the infrequently studied genus Chryseobacterium. The proportions of bacteria cultured from head lettuce and romaine leaves that produce biosurfactants were directly correlated with the culture-independent proportion of pseudomonads in a given sample, whereas spinach harbored a wider diversity of biosurfactant producers. A subset of the culturable bacteria in irrigation water also became enriched on romaine leaves that were irrigated overhead. Although our study was designed to identify surfactant producers on plants, we also provide evidence that most bacteria in some habitats, such as agronomic plant surfaces, are culturable, and these communities can be readily investigated and described by more classical culturing methods. IMPORTANCEThe importance of biosurfactant production to the bacteria that live on waxy leaf surfaces as well as their ability to be accurately assessed using culture-based methodologies was determined by interrogating epiphytic populations by both culture-dependent and culture-independent methods. Biosurfactant production was much more frequently observed in cultured communities on leaves than in other nearby habitats, such as soil and water, suggesting that this trait is important to life on a leaf by altering either the leaf itself or the interaction of bacteria with water. While pseudomonads were the most common biosurfactant producers isolated, this habitat also selects for taxa, such as Chryseobacterium, for which this trait was previously unrecognized. The finding that most epiphytic bacterial taxa were culturable validates strategies using more classical culturing methodologies for their study in this habitat. The phyllosphere is recognized to be a selective habitat in which epiphytic bacteria must be able to access limited and spatially heterogeneous nutrient supplies and endure frequent fluctuations in moisture availability on a water-repellent surface (1, 2). Recent culture-independent community analyses have confirmed the apparent selectivity of leaf surfaces, finding that leaf sur...

show abstract

mentioning

confidence: 99%

High-Level Culturability of Epiphytic Bacteria and Frequency of Biosurfactant Producers on Leaves

Burch

Paulina

Sbodio

et al. 2016

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Published reports show plant and clinical strains of Pseudomonas (e.g., Pseudomonas putida, P. fluorescens, and other Pseudomonas spp.) produce secondary antimicrobial metabolites, including enzymes, volatiles (hydrogen cyanide), cyclic lipopeptides, and antibiotics (33)(34)(35). These have been applied in plant pathology to control fungal pathogens and in clinical studies to inhibit pathogenic strains (36)(37)(38).…”

Section: Discussionmentioning

confidence: 99%

Interstrain Interactions between Bacteria Isolated from Vacuum-Packaged Refrigerated Beef

Zhang

Baranyi

Tamplin

2015

Appl Environ Microbiol

View full text Add to dashboard Cite

bThe formation of bacterial spoilage communities in food is influenced by both extrinsic and intrinsic environmental factors. Although many reports describe how these factors affect bacterial growth, much less is known about interactions among bacteria, which may influence community structure. This study investigated interactions among representative species of bacteria isolated from vacuum-packaged (VP) beef. Thirty-nine effectors and 20 target isolates were selected, representing 10 bacterial genera: Carnobacterium, Pseudomonas, Hafnia, Serratia, Yersinia, Rahnella, Brochothrix, Bacillus, Leuconostoc, and Staphylococcus. The influence of live effectors on growth of target isolates was measured by spot-lawn agar assay and also in liquid culture medium broth using live targets and effector cell-free supernatants. Inhibition on agar was quantified by diameter of inhibition zone and in broth by measuring detection time, growth rate, and maximum population density. A number of interactions were observed, with 28.6% of isolates inhibiting and 4.2% promoting growth. The majority of Pseudomonas isolates antagonized growth of approximately one-half of target isolates. Two Bacillus spp. each inhibited 16 targets. Among lactic acid bacteria (LAB), Carnobacterium maltaromaticum inhibited a wider range of isolates compared to other LAB. The majority of effector isolates enhancing target isolate growth were Gram-negative, including Pseudomonas spp. and Enterobacteriaceae. These findings markedly improve the understanding of potential interactions among spoilage bacteria, possibly leading to more mechanistic descriptions of bacterial community formation in VP beef and other foods. The shelf-life of meat is influenced, in part, by the composition and levels of bacteria within the spoilage community (1). Independent laboratories have confirmed relatively high microbial diversity at the time of meat packaging, and showing a progressive shift to lower community complexity toward the end of shelf-life (2-4). For refrigerated vacuum-packaged (VP) beef, over time and under best-practice conditions, lactic acid bacteria (LAB) tend to predominate and, to a lesser extent, Enterobacteriaceae (5).Such change in bacterial community structure is based on intrinsic and extrinsic factors, including temperature, atmosphere, pH, and organic acids, all of which may influence growth (5, 6). However, the underlying forces of microbial interactions may also be important in shaping biodiversity of communities (7-10); such studies have received relatively little attention in foods. Bacteria interact in any given ecological niche through different mechanisms, including quorum sensing, contact-dependent inhibition, and nutrient competition, and via production of defense compounds such as bacteriocins, antibiotics, and organic acids (10-14). There have been numerous reports exploring the effectiveness of protective cultures and related antibacterial compounds at enhancing food safety and extending shelf-life (15-18); however, few have investigated int...

show abstract

“…Infante-Duarte et al [35] demonstrated that the lipopeptides derived from B. burgdorferi stimulate the IL-17 expression (Cytokine produced by activated murine cytotoxic T-cells) in both murine and human T H cells. They induce the IL-17 expression together with TNF-α, so are coexpressed with other proinflammatory cytokines.…”

Section: Lipopeptides As Immunomodulatorsmentioning

confidence: 99%

Lipopeptides: Biosynthesis and Applications

Walia¹

2015

J Microb Biochem Technol

View full text Add to dashboard Cite

Microbial surfactants are amphiphilic molecules. They possess a strong tendency to reduce the interfacial tension among compounds. Biosurfactants possess diverse chemical structures and are nontoxic and hence are biodegradable. They have many applications in bioremediation, enhanced oil recovery, pharmaceutical and in food industry. In addition, biosurfactants have antifungal, antibacterial and antiviral properties. They are also found to have many therapeutic applications which as a result are very helpful for mankind in eradicating many pathogenic diseases. This review depicts the applications of lipopeptide biosurfactants in various fields.

show abstract

Functional, genetic and chemical characterization of biosurfactants produced by plant growth-promotingPseudomonas putida267

Cited by 107 publications

References 37 publications

High-Level Culturability of Epiphytic Bacteria and Frequency of Biosurfactant Producers on Leaves

High-Level Culturability of Epiphytic Bacteria and Frequency of Biosurfactant Producers on Leaves

Interstrain Interactions between Bacteria Isolated from Vacuum-Packaged Refrigerated Beef

Lipopeptides: Biosynthesis and Applications

Contact Info

Product

Resources

About