Functional Genomic Analysis of Sex Determination and Differentiation in Teleost Fish

Piferrer, Francesc; Martı́nez, Paulino; Ribas, Laia; Viñas, Ana; Díaz, Noèlia

doi:10.1002/9781118350041.ch8

Cited by 3 publications

(1 citation statement)

References 195 publications

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“…Although the many mysteries and complexities involved in the processes of development are far from solved, we now know that these processes involve widely distributed interactions across many levels of the individual and its environment. Scientists working with species as divergent as fruit flies, angler fish, macaques, and humans have provided compelling evidence over the last several decades that the development of any physical or behavioral trait is the result of a complex web of co‐actions among the organism's genes, molecular interactions within and across cells, and the nature and sequence of the physical, biological, and social environments in which the individual develops.…”

Section: Introductionmentioning

confidence: 99%

Developmental evolution

Lickliter

2016

WIRES Cognitive Science

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Biologists and psychologists are re-thinking the long-standing premise of genes as the primary cause of development, a view widely embraced in 20th-century biology. This shift in thinking is based in large part on: (1) the growing appreciation of the complex, distributed regulatory dynamics of gene expression; and (2) the growing appreciation of the probabilistic, contingent, and situated nature of development. We now appreciate that what actually unfolds during individual development represents only one of many possibilities. This expanded focus on the developmental process, often referred to as a developmental systems approach, has far-reaching implications for developmental and evolutionary theory, including new ways of thinking about the consequences of activity and experience, the emergence of novel properties or traits, the nature and extent of heredity, and the origins of phenotypic variability. WIREs Cogn Sci 2017, 8:e1422. doi: 10.1002/wcs.1422 For further resources related to this article, please visit the WIREs website.

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Section: Introductionmentioning

confidence: 99%

Developmental evolution

Lickliter

2016

WIRES Cognitive Science

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Reproduction and Sex Control in Turbot

Taboada

Robledo

Bouza

et al. 2018

Sex Control in Aquaculture

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Multiple evidences suggest sox2 as the main driver of a young and complex sex determining ZW/ZZ system in turbot (Scophthalmus maximus)

Martı́nez

Robledo

Taboada

et al. 2019

Preprint

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A major challenge in evolutionary biology is to find an explanation for the variation in sex-determining (SD) systems across taxa and to understand the mechanisms driving sex chromosome differentiation. We studied the turbot, holding a ZW/ZZ SD system and no sex chromosome heteromorphism, by combining classical genetics and genomics approaches to disentangle the genetic architecture of this trait. RAD-Seq was used to genotype 18,214 SNPs on 1,135 fish from 36 families and a genome wide association study (GWAS) identified a ~ 6 Mb region on LG5 associated with sex (P < 0.05). The most significant associated markers were located close to sox2, dnajc19 and fxr1 genes. A segregation analysis enabled narrowing down the associated region and evidenced recombination suppression in a region overlapping the candidate genes. A Nanopore/Illumina assembly of the SD region using ZZ and WW individuals identified a single SNP fully associated with Z and W chromosomes. RNA-seq from 5-90 day-old fish detected the expression along the gonad differentiation period of a short non-coding splicing variant (ncRNA) included in a vertebrate-conserved long non-coding RNA overlapping sox2. qPCR showed that sox2 was the only differentially expressed gene between males and females at 50-55 days post fertilization, just prior the beginning of gonad differentiation. More refined information on the involvement of secondary genetic and environmental factors and their interactions on SD was gathered after the analysis of a broad sample of families. Our results confirm the complex nature of SD in turbot and support sox2 as its main driver.

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Functional Genomic Analysis of Sex Determination and Differentiation in Teleost Fish

Cited by 3 publications

References 195 publications

Developmental evolution

Developmental evolution

Reproduction and Sex Control in Turbot

Multiple evidences suggest sox2 as the main driver of a young and complex sex determining ZW/ZZ system in turbot (Scophthalmus maximus)

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