Functional Genomics Approach Towards Dissecting Out Abiotic Stress Tolerance Trait in Plants

Joshi, Rohit; Gupta, B. K.; Pareek, Ashwani; Singh, Mohan B.; Singla‐Pareek, Sneh L.

doi:10.1007/978-3-319-91956-0_1

Cited by 4 publications

(4 citation statements)

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“…Functional studies of genomes readily produce information that is applicable to crop improvement. Functional genomics involves the functional characterization of genes and their interactions with other genes in a regulatory network [10]. Functional genomics includes different approaches to identify gene functions, such as sequence-or hybridizationbased methodologies, gene inactivation or editing-based approaches, and gene overexpression [10].…”

Section: Functional Genomicsmentioning

confidence: 99%

Genomic and Transcriptomic Approaches to Developing Abiotic Stress-Resilient Crops

Kamali,

Singh

2023

Agronomy

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In the realm of agriculture, a pressing concern remains the abiotic stresses, such as temperature fluctuation, drought, soil salinity, and heavy metal contamination. These adverse growth conditions hamper crop yields and global food security. In this review, we present a comprehensive examination of the recent advancements in utilizing genomics and transcriptomics, tools to enhance crop resilience against these stress factors. Genomics aids in the identification of genes responsive to stress, unravels regulatory networks, and pinpoints genetic variations linked to stress tolerance. Concurrently, transcriptomics sheds light on the intricate dynamics of gene expression during stress conditions, unearthing novel stress-responsive genes and signaling pathways. This wealth of knowledge shapes the development of stress-tolerant crop varieties, achieved through conventional breeding programs and state-of-the-art genetic engineering and gene editing techniques like CRISPR-Cas9. Moreover, the integration of diverse omics data and functional genomics tools empowers precise manipulation of crop genomes to fortify their stress resilience. In summary, the integration of genomics and transcriptomics holds substantial promise in elucidating the molecular mechanisms behind crop stress tolerance, offering a path towards sustainable agriculture and safeguarding food security amidst shifting environmental challenges.

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Section: Functional Genomicsmentioning

confidence: 99%

Genomic and Transcriptomic Approaches to Developing Abiotic Stress-Resilient Crops

Kamali,

Singh

2023

Agronomy

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“…It has discovered that functional genomic investigations easily yield information useful for crop development. Characterizing how genes function and how they interact with other genes in regulatory networks is a component of functional genomics ( Joshi et al., 2019 ). New genes and their functions has found thanks to recent developments in biotechnological methods.…”

Section: Functional Genomicsmentioning

confidence: 99%

“…New genes and their functions has found thanks to recent developments in biotechnological methods. In functional genomics, techniques based on sequencing, hybridization and gene inactivation or editing are all employed ( Joshi et al., 2019 ). Sequence-based techniques include EST (expressed sequence tags), SAGE (serial analysis of gene expression) and 50RACE (rapid amplification of cDNA ends).…”

Section: Functional Genomicsmentioning

confidence: 99%

Genomics and transcriptomics to protect rice (Oryza sativa. L.) from abiotic stressors: -pathways to achieving zero hunger

Ahmad

2022

Front. Plant Sci.

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More over half of the world’s population depends on rice as a major food crop. Rice (Oryza sativa L.) is vulnerable to abiotic challenges including drought, cold, and salinity since it grown in semi-aquatic, tropical, or subtropical settings. Abiotic stress resistance has bred into rice plants since the earliest rice cultivation techniques. Prior to the discovery of the genome, abiotic stress-related genes were identified using forward genetic methods, and abiotic stress-tolerant lines have developed using traditional breeding methods. Dynamic transcriptome expression represents the degree of gene expression in a specific cell, tissue, or organ of an individual organism at a specific point in its growth and development. Transcriptomics can reveal the expression at the entire genome level during stressful conditions from the entire transcriptional level, which can be helpful in understanding the intricate regulatory network relating to the stress tolerance and adaptability of plants. Rice (Oryza sativa L.) gene families found comparatively using the reference genome sequences of other plant species, allowing for genome-wide identification. Transcriptomics via gene expression profiling which have recently dominated by RNA-seq complements genomic techniques. The identification of numerous important qtl,s genes, promoter elements, transcription factors and miRNAs involved in rice response to abiotic stress was made possible by all of these genomic and transcriptomic techniques. The use of several genomes and transcriptome methodologies to comprehend rice (Oryza sativa, L.) ability to withstand abiotic stress have been discussed in this review

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“…Para la utilización de esas técnicas es necesario que se hayan identificado y caracterizado molecularmente los genes que estén relacionados con la respuesta al estrés. Entre estos genes están los afines con el transporte de iones y agua, la síntesis de sustancias osmoprotectoras como glicinabetaína, manitol y prolina, transportadores de exclusión de iones, mecanismos para la detoxificación de especies de oxígeno reactivo (ROS), producción de moléculas chaperonas y controles de la regulación transcripcional (Joshi et al, 2019;Parmar et al, 2017;Patel et al, 2019).…”

Section: Introductionunclassified

Ingeniería genética contra estrés abiótico en cultivos neotropicales: osmolitos, factores de transcripción y CRISPR/Cas9

Jiménez

Carvajal-Campos

2021

Rev. colomb. biotecnol.

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El neotrópico es sitio de origen de gran variedad de plantas que actualmente son cultivadas con éxito en diferentes regiones del mundo. Sin embargo, condiciones climáticas adversas, que se pueden ver acrecentadas por efectos del cambio climático antropogénico, pueden afectar su rendimiento y productividad debido a las situaciones de estrés abiótico que se pueden generar. Como alternativa para contrarrestar estos efectos, se ha experimentado con modificaciones genéticas, particularmente en genes relacionados con la producción de osmolitos y factores de transcripción que han llevado a que estas plantas, a nivel experimental, tengan mayor tolerancia a estrés oxidativo, altas y bajas temperaturas y fotoinhibición, sequía y salinidad, mediante la acumulación de osmoprotectores, la regulación en la expresión de genes y cambios en el fenotipo. En este trabajo se presentan y describen las estrategias metodológicas planteadas con estos fines y se complementan con ejemplos de trabajos realizados en cultivos de origen neotropical de importancia económica, como maíz, algodón, papa y tomate. Además, y debido a la novedad y potencial que ofrece la edición génica por medio del sistema CRISPR/Cas9, también se mencionan trabajos realizados en plantas con origen neotropical, enfocados en comprender e implementar mecanismos de tolerancia a sequía. Las metodologías aquí descritas podrían constituirse en opciones prácticas para mejorar la seguridad alimentaria con miras a contrarrestar las consecuencias negativas del cambio climático antropogénico.

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Functional Genomics Approach Towards Dissecting Out Abiotic Stress Tolerance Trait in Plants

Cited by 4 publications

References 158 publications

Genomic and Transcriptomic Approaches to Developing Abiotic Stress-Resilient Crops

Genomic and Transcriptomic Approaches to Developing Abiotic Stress-Resilient Crops

Genomics and transcriptomics to protect rice (Oryza sativa. L.) from abiotic stressors: -pathways to achieving zero hunger

Ingeniería genética contra estrés abiótico en cultivos neotropicales: osmolitos, factores de transcripción y CRISPR/Cas9

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