Functional genomics assessment of narcotic and specific acting chemical pollutants using E. coli

Guan, Miao; Fang, Wendi; Ullah, Sana; Zhang, Xiaowei; Saquib, Quaiser; Al-Khedhairy, Abdulaziz A.

doi:10.1016/j.envpol.2017.09.027

Cited by 9 publications

(2 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to its use in the determination of underlying toxicological mechanisms, functional genome screening could also be valuable in mechanism-based chemical classification. In our recent studies using functional genomics screening with Escherichia coli , a group of four genes ( flhC, fliN , fliH , and flhD ) that are associated with motility and the ability to form biofilms, was capable of distinguishing substances with narcotic action from specifically acting substances …”

Section: Using Functional Genomics To Study Novel Molecular Mechanism...mentioning

confidence: 99%

“…In our recent studies using functional genomics screening with Escherichia coli, a group of four genes (f lhC, f liN, f liH, and f lhD) that are associated with motility and the ability to form biofilms, was capable of distinguishing substances with narcotic action from specifically acting substances. 20…”

Section: Using Functional Genomics To Study Novel Molecular Mechanism...mentioning

confidence: 99%

See 1 more Smart Citation

Omics Advances in Ecotoxicology

Zhang

Xia

Wang

et al. 2018

Environ. Sci. Technol.

Self Cite

145

View full text Add to dashboard Cite

Toxic substances in the environment generate adverse effects at all levels of biological organization from the molecular level to community and ecosystem. Given this complexity, it is not surprising that ecotoxicologists have struggled to address the full consequences of toxic substance release at ecosystem level, due to the limits of observational and experimental tools to reveal the changes in deep structure at different levels of organization. -Omics technologies, consisting of genomics and ecogenomics, have the power to reveal, in unprecedented detail, the cellular processes of an individual or biodiversity of a community in response to environmental change with high sample/observation throughput. This represents a historic opportunity to transform the way we study toxic substances in ecosystems, through direct linkage of ecological effects with the systems biology of organisms. Three recent examples of -omics advance in the assessment of toxic substances are explored here: (1) the use of functional genomics in the discovery of novel molecular mechanisms of toxicity of chemicals in the environment; (2) the development of laboratory pipelines of dose-dependent, reduced transcriptomics to support high-throughput chemical testing at the biological pathway level; and (3) the use of eDNA metabarcoding approaches for assessing chemical effects on biological communities in mesocosm experiments and through direct observation in field monitoring. -Omics advances in ecotoxicological studies not only generate new knowledge regarding mechanisms of toxicity and environmental effect, improving the relevance and immediacy of laboratory toxicological assessment, but can provide a wholly new paradigm for ecotoxicology by linking ecological models to mechanism-based, systems biology approaches.

show abstract

Section: Using Functional Genomics To Study Novel Molecular Mechanism...mentioning

confidence: 99%

Section: Using Functional Genomics To Study Novel Molecular Mechanism...mentioning

confidence: 99%