Functional gold nanoparticles coupled with laser desorption ionization mass spectrometry for bioanalysis

Unnikrishnan, Binesh; Chang, Chia-Yin; Chu, Han‐Wei; Anand, Anisha; Huang, Chih‐Ching

doi:10.1039/c6ay02378a

Cited by 36 publications

(31 citation statements)

References 70 publications

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“…To overcome the ion suppression of the targeted analytes or to analyze low abundant species, monitoring the signals produced by the desorption/ionization of the surface ligands and/or nanosubstrate cluster ions is usually more convenient. This indirect targeted approach provides higher sensitivity relative to the direct approach consisting in analyzing the signal belonging to the analytes (Unnikrishnan et al, 2016). In the "mass-tag" approach, the nanosubstrates are functionalized to bind specifically with a molecule of interest (e.g., proteins, lipids, membrane receptor,…).…”

Section: Mass-tag Reportersmentioning

confidence: 99%

Surface‐assisted laser desorption/ionization mass spectrometry imaging: A review

Müller

Verdin

Pauw

et al. 2020

Mass Spectrometry Reviews

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In the last decades, surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) has attracted increasing interest due to its unique capabilities, achievable through the nanostructured substrates used to promote the analyte desorption/ionization. While the most widely recognized asset of SALDI-MS is the untargeted analysis of small molecules, this technique also offers the possibility of targeted approaches. In particular, the implementation of SALDI-MS imaging (SALDI-MSI), which is the focus of this review, opens up new opportunities. After a brief discussion of the nomenclature and the fundamental mechanisms associated with this technique, which are still highly controversial, the

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Section: Mass-tag Reportersmentioning

confidence: 99%

Surface‐assisted laser desorption/ionization mass spectrometry imaging: A review

Müller

Verdin

Pauw

et al. 2020

Mass Spectrometry Reviews

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“…and chemical (surface modification, binding energy to analytes, etc.) properties of the nanomaterials 18–23 . Gold nanoparticles (AuNP) exhibit excellent electrical conductivities, thermal and chemical stabilities and strong light absorption due to plasmonic effects.…”

Section: Introductionmentioning

confidence: 99%

Influence of core size and capping ligand of gold nanoparticles on the desorption/ionization efficiency of small biomolecules in AP‐SALDI‐MS

Liu

Zhang

Pyttlik

et al. 2020

Analytical Science Advances

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Gold nanoparticles (AuNP) are frequently used in surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) for analysis of biomolecules because they exhibit suitable thermal and chemical properties as well as strong surface plasmonic effects. Moreover, the structures of AuNP can be controlled by well-established synthesis protocols. This was important in the present work, which studied the influence of the nanoparticles' structures on atmospheric pressure (AP)-SALDI-MS performance. A series of AuNP with different core sizes and capping ligands were investigated, to examine the desorption/ionization efficiency (DIE) under AP-SALDI conditions. The results showed that both the AuNP core size as well as the nature of the surface ligand had a strong influence on DIE. DIE increased with the size of the AuNP and the hydrophobicity of the ligands. Chemical interactions between ligand and analytes also influenced DIE. Moreover, we discovered that removing the organic ligands from the deposited AuNP substrate layer by simple laser irradiation prior to LDI further amplified DIE values. The optimized AuNP were successfully used to analyze a wide arrange of different low molecular weight biomolecules as well as a crude pig brain extract, which readily demonstrated the ability of the technique to detect a wide range of lipid species within highly complex samples. 1 INTRODUCTION Since cobalt nanoparticles were first used for surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) by Tanaka et al, 1 nanomaterials made from carbon, 2-4 silicon, 5-7 metals (Au, Ag, Pa, etc.), 8-10 metal oxides, 11-13 and others materials 14-17 have been successfully applied to SALDI-MS. In general, nanoparticles are excellent substrate options for SALDI, because they require only simple and easy sample preparation techniques and the materials often exhibit minimal background signals-in particular in the low m/z range-as compared to matrix-assisted laser desorption/ionization (MALDI). 18-20 SALDI efficiencies can be enhanced by optimizing the nanoparticular structures, for example, by modifying the core size and nature of the surface ligands, 18 which was shown to be vital for sensitive quantitative analysis of biomolecules. 20 In previous studies, it was found that desorption/ionization efficiency of SALDI was highly dependent on physical (size, surface roughness, light absorption, electrical conductivity, melting point, etc.) and chemical (surface modification, binding energy to analytes, etc.) properties of the nanomaterials. 18-23 Gold nanoparticles (AuNP) exhibit excellent electrical conductivities, thermal and chemical stabilities and strong light This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. c

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“…Surface-assisted laser desorption/ionisation mass spectrometry (SALDI-MS) is probably the most sensitive method of detecting residual CTAB on AuNRs, since quaternary ammonium ions have a strong capacity to be desorbed and ionised in LDI-MS. 24,25 The ionisation principle of SALDI is similar to that of matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-MS), [26][27][28] with the UV-absorbing organic matrix commonly used in MALDI replaced by UV-absorbing nanomaterials such as gold nanoparticles. 28 The organic matrix-free ionisation technique of SALDI-MS can avoid interference caused by organic matrix molecules in the low-mass region (below 500 Da).…”

Section: 2425mentioning

confidence: 99%

“…In particular, SALDI-MS allows highly sensitive detection of analytes adsorbed on the surface of gold nanoparticles via energy transfer from gold to the analyte. [26][27][28] For instance, Liz-Marzán and co-workers demonstrated the presence of CTAB on PEG-AuNRs by SALDI-MS. 24 Schulz and coworkers reported that although CTAB could not be identied in puried samples of PEG-AuNRs by nuclear magnetic resonance and X-ray photoelectron spectroscopy (XPS), the presence of residual CTA ions could be conrmed by SALDI-MS. 25 Thus, the development of an effective strategy for removing surfacebound CTA cations from PEG-AuNRs is highly challenging.…”

Section: 2425mentioning

confidence: 99%

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Effective removal of surface-bound cetyltrimethylammonium ions from thiol-monolayer-protected Au nanorods by treatment with dimethyl sulfoxide/citric acid

Nishida

Kawasaki

2017

RSC Adv.

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Cetyltrimethylammonium bromide (CTAB)-based surfactants are typically used as morphology-directing/ stabilising agents for gold nanorods (AuNRs), forming bilayers on their surface. However, the biological applications of AuNRs require the removal of surface-bound CTAB due its high toxicity and the poor colloidal stability of CTAB-covered AuNRs in biological media. Herein, we report a simple and effective strategy for removing surface-bound cetyltrimethylammonium (CTA) cations from poly(ethylene glycol)thiolate-protected AuNRs (PEG-AuNRs) by treatment with dimethyl sulfoxide/citric acid (DMSO/Cit), achieving residual CTA ion levels that cannot be detected by highly sensitive mass spectrometry or X-ray photoelectron spectroscopy (XPS) techniques. The DMSO/Cit treatment is thought to destabilise the Ag-Br-CTA complex on AuNRs, since citric acid forms strongly bound chelate complexes with CTA cations.

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Functional gold nanoparticles coupled with laser desorption ionization mass spectrometry for bioanalysis

Abstract: Monitoring of surface ligand and gold cluster ions produced by pulsed-laser-induced desorption/ionization of gold nanoparticles enables the transduction and amplification of mass signals for biosensing and bioimaging applications.

Cited by 36 publications

References 70 publications

Surface‐assisted laser desorption/ionization mass spectrometry imaging: A review

Surface‐assisted laser desorption/ionization mass spectrometry imaging: A review

Influence of core size and capping ligand of gold nanoparticles on the desorption/ionization efficiency of small biomolecules in AP‐SALDI‐MS

Effective removal of surface-bound cetyltrimethylammonium ions from thiol-monolayer-protected Au nanorods by treatment with dimethyl sulfoxide/citric acid

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