Functional Identification of Corynespora cassiicola-Responsive miRNAs and Their Targets in Cucumber

Wang, Xiangyu; Yu, Guangchao; Zhao, Jing; Cui, Na; Yu, Yang; Fan, Haiyan

doi:10.3389/fpls.2019.00668

Cited by 12 publications

(5 citation statements)

References 67 publications

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“…Boeremia is thought to cause soybean black spot in Australia ( Jones et al, 2011 ). Corynespora could cause cucumber brown spot ( Wang et al, 2019 ). Exophiala is associated with the soybean cyst nematode ( Hu et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Effect of No Tillage System on Soil Fungal Community Structure of Cropland in Mollisol: A Case Study

Gao

2022

Front. Microbiol.

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Conservation tillage is generally regarded as a sustainable farming system for the future. The fungal community structure has a strong response to conservation tillage. However, how the conservation tillage system affects the soil fungal community structure is little known. Using the high-throughput sequencing technology, the soil fungal community was explored under no tillage (NT) and conventional tillage (CT) in Northeast China Mollisol. The copy number of fungal genes in NT20 was significantly lower than that in other treatments. NT changed the composition of soil fungal communities at the taxonomic level of phylum and genus. The diversity indices of the soil fungal community in no tillage at soil depths of 0–5 cm (NT5) were significantly higher than those in soil depths of 5–20 cm (NT20). The fungal community under NT and CT could form a good cluster distribution and NT5, conventional tillage at soil depths of 0–5 cm (CT5) and 5–20 cm (CT20) had specific indicator species. Most of the potential pathogens were significantly higher in NT5 than in NT20. Tillage and soil depth could explain 64% of the diversity and 95% of the composition of the fungal community, which indirectly changed the diversity and composition of fungi by using soil organic carbon, pH value, and soil bulk density. Furthermore, soil organic carbon (SOC) best explained the soil fungal community, followed by soil pH. The study indicated that the NT system had a comprehensive effect on the soil fungal community and SOC is the most crucial factor in determining this community.

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Section: Discussionmentioning

confidence: 99%

Effect of No Tillage System on Soil Fungal Community Structure of Cropland in Mollisol: A Case Study

Gao

2022

Front. Microbiol.

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“…The transient expression of tobacco via Agrobacterium mediated is an extremely well known and reliable method for gene interaction study in plants (Wang et al, 2019; Yin et al, 2017). This method is generally used to examine the expression of the GUS genes in tobacco leaves following the transient expression of miRNAs and target genes (Han et al, 2016; Rao et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…The confirmed target gene was transformed to binary vector pCAMBIA1304. Finally, all the three constructs were transformed into the Agrobacterium tumefaciens EHA105 strain (Wang et al, 2019). Further, the constructs were transiently expressed in 28 days old Nicotiana benthamiana ( N. benthamiana ) leaves using an A. tumefaciens transformation protocol (Rao et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

Identification and analysis of miRNAs‐lncRNAs‐mRNAs modules involved in stem‐elongation of deepwater rice (Oryza sativa L.)

Panda

Rawal

Jain

et al. 2022

Physiologia Plantarum

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Deepwater is an abiotic stress that limits rice cultivation worldwide due to recurrent floods. The miRNAs and lncRNAs are two non‐coding RNAs emerging as major regulators of gene expressions under different abiotic stresses. However, the regulation of these two non‐coding RNAs under deepwater stress in rice is still unexplored. In this study, small RNA‐seq and RNA‐seq from internode and node tissues were analyzed to predict deepwater stress responsive miRNAs and lncRNAs, respectively. Additionally, a competitive endogenous RNA (ceRNA) study revealed about 69 and 25 lncRNAs acting as endogenous target mimics (eTM) with the internode and node miRNAs, respectively. In ceRNA analyses, some of the key miRNAs such as miR1850.1, miR1848, and IN‐nov‐miR145 were upregulated while miR159e was downregulated, and their respective eTM lncRNAs and targets were found to have opposite expressions. Moreover, we have transiently expressed one module (IN‐nov‐miR145–Cc‐TCONS_00011544‐Os11g36430.3) in tobacco leaves. The integrated analysis has identified differentially expressed (DE) miRNAs, lncRNAs and their target genes, and the complex regulatory network, which might lead to stem elongation under deepwater stress. In this novel attempt to identify and characterize miRNAs and lncRNAs under deepwater stress in rice, we have provided, probably for the first time, a reference platform to study the interactions of these two non‐coding RNAs with respective target genes through transient expression analyses.

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“…The activity levels of these enzymes are positively correlated with fruit disease resistance and could be used as indicators for inducing fruit disease resistance levels. PAL is a crucial enzyme in the phenylpropanoid metabolic pathway, which is essential for normal growth, development and resistance of plants ( Qin et al, 2015 ; Wang et al, 2019a ). In plant disease resistance, PAL can promote the production of secondary metabolites, including total phenols, flavonoids and lignin ( Romanazzi et al, 2016 ; Wang et al, 2016 ; Ge et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Impact of vanillin on postharvest disease control of apple

et al. 2022

Self Cite

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Apple fruits are susceptible to infection by postharvest fungal pathogens, which may cause fruit decay and severe economic losses. This study investigated the antifungal spectrum of vanillin against common decay pathogens of apple and explored the antifungal mechanisms of vanillin in vitro. In vivo experiments were carried out to evaluate the effects of vanillin on apple postharvest disease control and fruit quality. Moreover, the induced resistance mechanism of vanillin on apple fruit was preliminarily explored. The results showed that vanillin has broad-spectrum antifungal effects, especially on Alternaria alternata. Vanillin could significantly inhibit the growth rate, mycelium biomass, and spore germination of pathogenic fungi by increasing the cell membrane permeability and lipid peroxidation. Importantly, vanillin treatment reduced the incidence of apple decay caused by A. alternata and Penicillium expansum, and contributed to improve fruit quality. Further studies indicated that vanillin could induce elevation in the activities of defense-related enzymes in apple fruit, such as phenylalanine ammonia-lyase (PAL), chitinase (CHI) and β-1,3-glucanase (β-1,3-GA), and increase total phenols and flavonoids contents. Generally, these results suggest that vanillin may contribute to the induced resistance of apple fruits to pathogenic fungi. To conclude, the results of this research provide theoretical foundations for the application of vanillin in the control of apple postharvest decay caused by fungal pathogens.

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Functional Identification of Corynespora cassiicola-Responsive miRNAs and Their Targets in Cucumber

Cited by 12 publications

References 67 publications

Effect of No Tillage System on Soil Fungal Community Structure of Cropland in Mollisol: A Case Study

Effect of No Tillage System on Soil Fungal Community Structure of Cropland in Mollisol: A Case Study

Identification and analysis of miRNAs‐lncRNAs‐mRNAs modules involved in stem‐elongation of deepwater rice (Oryza sativa L.)

Impact of vanillin on postharvest disease control of apple

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