Functional inhibition of UQCRB suppresses angiogenesis in zebrafish

Cho, Yoon Sun; Jung, Hye Jin; Seok, Seung Hyeok; Payumo, Alexander Y.; Chen, James; Kwon, Ho Jeong

doi:10.1016/j.bbrc.2013.02.082

Cited by 28 publications

(15 citation statements)

References 23 publications

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“…Another oxidative phosphorylation-related DEG, UQCRB, encodes subunit 7 of ubiquinol cytochrome c reductase. UQCRB plays a key role in hypoxia-induced angiogenesis and mitochondria-mediated metabolic disorders (Jung et al 2011;Chang et al 2014;Chang et al 2015), and the loss of UQCRB function inhibits angiogenesis (Cho et al 2013). NDUFS6 encodes NADH, ubiquinone oxidoreductase and dehydrogenase (ubiquinone) Fe-S protein 6 (Loeffen et al 1998).…”

Section: Resultsmentioning

confidence: 99%

Cardiac transcriptome study of the effect of heat stress in yellow-feather broilers

Zhang

Luo

2019

Italian Journal of Animal Science

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The yellow-feather broiler is a popular poultry breed in Asia, particularly in China. In this study, we performed RNA-seq analysis to identify differentially expressed genes (DEGs) in the heart of yellow-feather broilers that had been subjected to heat stress treatment (38 ± 1 C for 8 h, over 7 d) and determine the response of the heart to high temperature and its effects on yellowfeather broiler physiology. We found that body weight (BW) of the heat stress treatment group (BW 28 ¼ 354.8 ± 34.8 g) was significantly decreased (p ¼ .033) compared with that of the control group (BW 28 ¼ 384.8 ± 58.9 g). However, there was no significant reduction in the heart relative weight (HRW) (p ¼ .538). A total of 37 DEGs related to energy metabolism responded to heat stress in the heart of yellow-feather broiler. The results of KEGG pathways analysis indicated that these genes are involved in oxidative phosphorylation (KO: 00190), cardiac muscle contraction (KO: 04260) and carbon metabolism (KO: 01200). Analysis of the cardiac transcriptome of yellowfeather broilers subjected to heat stress indicated that the heart of these birds has specific physiological mechanisms for regulating body growth in response to high-temperature environments. HIGHLIGHTS Yellow-feather broilers, a popular poultry breed in Asia, were used to determine how the heart responds to heat stress. A total of 37 genes in the heart of yellow-feather broilers showed differential expression in response to heat stress. The differentially expressed genes (DEGs) are associated with oxidative phosphorylation, cardiac muscle contraction and carbon metabolism pathways.

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Section: Resultsmentioning

confidence: 99%

Cardiac transcriptome study of the effect of heat stress in yellow-feather broilers

Zhang

Luo

2019

Italian Journal of Animal Science

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“…Of these, pyridaben, fenpyroxymate, tebufenpyrad, and rotenone inhibit Complex I of the electron transport chain, while pyraclostrobin and trifloxystrobin inhibit respiratory Complex III. Corroborating the importance of Complex III for angiogenesis, knock down or chemical inhibition of ubiquinol-cytochrome c reductase binding protein (Uqcrb), a Complex III subunit, in zebrafish inhibits ISV angiogenesis and suppresses VEGF levels [64]. It should be noted that inhibition of mitochondrial function is a relatively common mechanism of action for pesticides, and the correlation between mitochondrial disruptive activity and high VDC ranking might be ancillary.…”

Section: Discussionmentioning

confidence: 99%

Identification of vascular disruptor compounds by analysis in zebrafish embryos and mouse embryonic endothelial cells

McCollum

Conde‐Vancells

Hans

et al. 2017

Reproductive Toxicology

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To identify vascular disruptor compounds (VDCs), this study utilized an in vivo zebrafish embryo vascular model in conjunction with a mouse endothelial cell model to screen a subset of the U.S. Environmental Protection Agency (EPA) ToxCast Phase I chemical inventory. In zebrafish, 161 compounds were screened and 34 were identified by visual inspection as VDCs, of which 28 were confirmed as VDCs by quantitative image analysis. Testing of the zebrafish VDCs for their capacity to inhibit endothelial tube formation in the murine yolk-sac-derived endothelial cell line C166 identified 22 compounds that both disrupted zebrafish vascular development and murine endothelial in vitro tubulogenesis. Putative molecular targets for the VDCs were predicted using EPA’s Toxicological Prioritization Index tool and a VDC signature based on a proposed adverse outcome pathway for developmental vascular toxicity. In conclusion, our screening approach identified 22 novel VDCs, some of which were active at nanomolar concentrations

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“…Subsequent studies revealed that terpestacin suppresses angiogenesis by inhibiting the generation of ROS by complex III during hypoxia. The loss of the ROS signal in turn limits the hypoxia-induced stabilization of HIF-1α, a transcriptional driver of VEGF expression and angiogenesis 107 . At concentrations that suppress ROS generation, terpestacin did not inhibit cellular respiration or ATP generation.…”

Section: Further Evidence For Complex Iii-derived Ros In O2 Sensingmentioning

confidence: 99%

O 2 sensing, mitochondria and ROS signaling: The fog is lifting

Waypa

Smith

Schumacker

2016

Molecular Aspects of Medicine

163

121

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Mitochondria are responsible for the majority of oxygen consumption in cells, and thus represent a conceptually appealing site for cellular oxygen sensing. Over the past 40 years a number of mechanisms to explain how mitochondria participate in oxygen sensing have been proposed. However, no consensus has been reached regarding how mitochondria could regulate transcriptional and post-translational responses to hypoxia. Nevertheless, a growing body of data continues to implicate a role for increased reactive oxygen species (ROS) signals from the electron transport chain (ETC) in triggering responses to hypoxia in diverse cell types. The present article reviews our progress understanding this field and considers recent advances that provide new insight, helping to lift the fog from this complex topic.

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Functional inhibition of UQCRB suppresses angiogenesis in zebrafish

Cited by 28 publications

References 23 publications

Cardiac transcriptome study of the effect of heat stress in yellow-feather broilers

Cardiac transcriptome study of the effect of heat stress in yellow-feather broilers

Identification of vascular disruptor compounds by analysis in zebrafish embryos and mouse embryonic endothelial cells

O 2 sensing, mitochondria and ROS signaling: The fog is lifting

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