Functional Living Biointerphases

Saadeddin, Anas; Rodrigo‐Navarro, Aleixandre; Monedero, Vicente; Rico, Patricia; Moratal, David; González-Martı́n, M.L.; Navarro, David; Garcı́a, Andrés J.; Salmerón‐Sánchez, Manuel

doi:10.1002/adhm.201200473

Cited by 15 publications

(25 citation statements)

References 30 publications

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“…lactis develops stable biofilms, while keeping high viability values [216]. Bacteria shows high viability levels even with low concentrations of tetracycline, a bacteriostatic antibiotic.…”

Section: Discussionmentioning

confidence: 99%

“…[14,15,295] From a different perspective, dynamic cell microenvironments have been engineered to present and release growth factors in a sustainable and controlled way to stimulate and support cell differentiation. [16,[296][297][298][299][300][301] As seen in Chapter 4, we have engineered [216] Lactococcus lactis to display the III 7−10 domains of human fibronectin as a membrane-bound protein. III 7−10 contains the RGD and PHSRN synergy sequences which promote cell adhesion and differentiation [31,302].…”

Section: Discussionmentioning

confidence: 99%

“…Several cell lines, namely fibronectin-null fibroblasts (Fn − /Fn − ), NIH3T3 and C2C12 adhere, spread and develop focal adhesion complexes on the engineered L. lactis biofilm, behaving in a similar way as when cultured directly on a FN-coated surface. [216] This strain produces stable biofilms that support cellular growth, adhesion and differentiation [211] . The formation of the biofilm starts when some individual cells adhere to the surface, a process governed by weak non-specific forces -Van der Waals and polar Lewis acid-base interactions [43,303].…”

Section: Discussionmentioning

confidence: 99%

“…With a size of 5706 bp, it has been successfully used as a membrane-anchored protein expression vector in L. lactis and other LAB. [61,215,216] Protein A (SpA) is a 42 kDa cell-wall anchored protein expressed by Staphylococcus aureus [212]; the anchoring mechanism includes a 35-residue sorting signal located at the C-terminal end of the protein A is required. SpA contains the LPETG motif followed by a C-terminal hydrophobic domain and tail composed of mainly positively charged residues.…”

Section: Lactis Why and Howmentioning

confidence: 99%

“…In this chapter, the use of Lactococcus lactis as a living biointerface [216] is presented. This bacterium has been genetically modified to express the FN-III 7−10 repeats of the human fibronectin, taking advantage of its low exopolysaccharide production, to make it accessible to the extracellular environment.…”

Section: Introductionmentioning

confidence: 99%

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Functional living biointerfaces to direct cell-material interaction

Navarro¹

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“…lactis develops stable biofilms, while keeping high viability values [216]. Bacteria shows high viability levels even with low concentrations of tetracycline, a bacteriostatic antibiotic.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Lactis Why and Howmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Functional living biointerfaces to direct cell-material interaction

Navarro¹

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Optoregulated Protein Release from an Engineered Living Material

Sankaran

Campo

2018

Adv. Biosys.

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Developing materials to encapsulate and deliver functional proteins inside the body is a challenging yet rewarding task for therapeutic purposes. High production costs, mostly associated with the purification process, short‐term stability in vivo, and controlled and prolonged release are major hurdles for the clinical application of protein‐based biopharmaceuticals. In an attempt to overcome these hurdles, herein, the possibility of incorporating bacteria as protein factories into a material and externally controlling protein release using optogenetics is demonstrated. By engineering bacteria to express and secrete a red fluorescent protein in response to low doses of blue light irradiation and embedding them in agarose hydrogels, living materials are fabricated capable of releasing proteins into the surrounding medium when exposed to light. These bacterial hydrogels allow spatially confined protein expression and dosed protein release over several weeks, regulated by the area and extent of light exposure. The possibility of incorporating such complex functions in a material using relatively simple material and genetic engineering strategies highlights the immense potential and versatility offered by living materials for protein‐based biopharmaceutical delivery.

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Bacteria‐Based Materials for Stem Cell Engineering

et al. 2018

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Materials can be engineered to deliver specific biological cues that control stem cell growth and differentiation. However, current materials are still limited for stem cell engineering as stem cells are regulated by a complex biological milieu that requires spatiotemporal control. Here a new approach of using materials that incorporate designed bacteria as units that can be engineered to control human mesenchymal stem cells (hMSCs), in a highly dynamic-temporal manner, is presented. Engineered Lactococcus lactis spontaneously colonizes a variety of material surfaces (e.g., polymers, metals, and ceramics) and is able to maintain growth and induce differentiation of hMSCs in 2D/3D surfaces and hydrogels. Controlled, dynamic, expression of fibronectin fragments supports stem cell growth, whereas inducible-temporal regulation of secreted bone morphogenetic protein-2 drives osteogenesis in an on-demand manner. This approach enables stem cell technologies using material systems that host symbiotic interactions between eukaryotic and prokaryotic cells.

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Functional Living Biointerphases

Cited by 15 publications

References 30 publications

Functional living biointerfaces to direct cell-material interaction

Functional living biointerfaces to direct cell-material interaction

Optoregulated Protein Release from an Engineered Living Material

Bacteria‐Based Materials for Stem Cell Engineering

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