Functional monoclonal antibodies to p75 neurotrophin receptor raised in knockout mice

Rogers, Mary‐Louise; Atmosukarto, Ines I.; Berhanu, Degu A.; Matusica, Dusan; Macardle, Peter J.; Rush, Robert A.

doi:10.1016/j.jneumeth.2006.05.022

Cited by 30 publications

(33 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We used a trk inhibitor K252a to examine the role of trkA, and a mixture of MLR2 and MLR3, monoclonal antibodies for p75 NTR , to test the role of p75 NTR . The MLR antibodies have been confirmed to bind rat p75 NTR and to inhibit the effects of NGF mediated through p75 NTR (Rogers et al, 2006). Two-way ANOVA and Bonferroni posttests revealed that K252a failed to reduce the NGF-induced increase in the amplitude of EPSCs in cholinergic neurons ( p Ͼ 0.05) (Fig.…”

Section: Mechanisms Of Ngf Actionmentioning

confidence: 89%

Chronic Exposure to Nerve Growth Factor Increases Acetylcholine and Glutamate Release from Cholinergic Neurons of the Rat Medial Septum and Diagonal Band of Broca via Mechanisms Mediated by p75^NTR

Huh¹,

Danik²,

Manseau³

et al. 2008

J. Neurosci.

View full text Add to dashboard Cite

Basal forebrain neurons play an important role in memory and attention. In addition to cholinergic and GABAergic neurons, glutamatergic neurons and neurons that can corelease acetylcholine and glutamate have recently been described in the basal forebrain. Although it is well known that nerve growth factor (NGF) promotes synaptic function of cholinergic basal forebrain neurons, how NGF affects the newly identified basal forebrain neurons remains undetermined. Here, we examined the effects of NGF on synaptic transmission of medial septum and diagonal band of Broca (MS-DBB) neurons expressing different neurotransmitter phenotypes. We used MS-DBB neurons from 10-to 13-d-old rats, cultured on astrocytic microislands to promote the development of autaptic connections. Evoked and spontaneous postsynaptic currents were recorded, and neurotransmitters released were characterized pharmacologically. We found that chronic exposure to NGF significantly increased acetylcholine and glutamate release from cholinergic MS-DBB neurons, whereas glutamate and GABA transmission from noncholinergic MS-DBB neurons were not affected by NGF. Interestingly, the NGF-induced increase in neurotransmission was mediated by p75NTR . These results demonstrate a previously unidentified role of NGF and its receptor p75 NTR ; their interactions are crucial for cholinergic and glutamatergic transmission in the septohippocampal pathway.

show abstract

Section: Mechanisms Of Ngf Actionmentioning

confidence: 89%

Chronic Exposure to Nerve Growth Factor Increases Acetylcholine and Glutamate Release from Cholinergic Neurons of the Rat Medial Septum and Diagonal Band of Broca via Mechanisms Mediated by p75^NTR

Huh¹,

Danik²,

Manseau³

et al. 2008

J. Neurosci.

View full text Add to dashboard Cite

show abstract

“…Unbound anti-CD20 antibodies were removed by centrifuging diatom preparations at 1,000g for 3 min at 8°C, followed by three subsequent 5 min washes in PBS at room temperature and finally dispersed in PBS. The same experiment was performed with the anti-p75NTR mouse monoclonal antibody (MLR2) 41 . Anti-p75NTR antibodies were kindly provided by Mary-Louise Rogers (Department of Human Physiology, Centre for Neuroscience, Flinders University, South Australia, Australia).…”

Section: Methodsmentioning

confidence: 99%

Targeted drug delivery using genetically engineered diatom biosilica

et al. 2015

Self Cite

View full text Add to dashboard Cite

The ability to selectively kill cancerous cell populations while leaving healthy cells unaffected is a key goal in anticancer therapeutics. The use of nanoporous silica-based materials as drug-delivery vehicles has recently proven successful, yet production of these materials requires costly and toxic chemicals. Here we use diatom microalgae-derived nanoporous biosilica to deliver chemotherapeutic drugs to cancer cells. The diatom Thalassiosira pseudonana is genetically engineered to display an IgG-binding domain of protein G on the biosilica surface, enabling attachment of cell-targeting antibodies. Neuroblastoma and B-lymphoma cells are selectively targeted and killed by biosilica displaying specific antibodies sorbed with drug-loaded nanoparticles. Treatment with the same biosilica leads to tumour growth regression in a subcutaneous mouse xenograft model of neuroblastoma. These data indicate that genetically engineered biosilica frustules may be used as versatile 'backpacks' for the targeted delivery of poorly water-soluble anticancer drugs to tumour sites.

show abstract

“…Rabbit anti-sortilin to the whole extracellular domain was kindly provided by Professor Claus Munck Petersen (Munck Petersen et al, 1999). The monoclonal anti-p75NTR from MLR2 hybridoma was purified as previously described (Rogers et al, 2006). Monoclonal p75NTR antibodies from ME20.4 and 8211 clones were from Biosensis and Millipore respectively and NGFR5 from BD Biosciences.…”

Section: Methodsmentioning

confidence: 99%

“…The cells were then incubated with the primary antibodies overnight at 4° C. In experiments aiming at detecting surface expression of the receptors, the cells were incubated overnight at 4° C with primary antibodies diluted in PBS-BSA without prior permeabilization. Primary antibodies used were rabbit anti-sortilin to the whole extracellular domain (1/400; (Munck Petersen et al, 1999), anti-trkA 5C3 (10 μg/ml; (LeSauteur et al, 1996) and anti-p75NTR MLR2 (20 μg/ml; (Rogers et al, 2006). Cells were then washed twice and incubated with Alexa-fluor 488 anti-rabbit or 647 anti-mouse (1/250) for 45 min at room temperature (Invitrogen).…”

Section: Methodsmentioning

confidence: 99%

ProNGF mediates death of Natural Killer cells through activation of the p75NTR–sortilin complex

Rogers

Bailey

Matusica

et al. 2010

Journal of Neuroimmunology

Self Cite

View full text Add to dashboard Cite

The common neurotrophin receptor P75NTR, its co-receptor sortilin and ligand proNGF, have not previously been investigated in Natural Killer (NK) cell function. We found freshly isolated NK cells express sortilin but not significant amounts of P75NTR unless exposed to interleukin-12 (IL-12), or cultured in serum free conditions, suggesting this receptor is sequestered. A second messenger associated with p75NTR, neurotrophin-receptor-interacting-MAGE-homologue (NRAGE) was identified in NK cells. Cleavage-resistant proNGF123 killed NK cells in the presence of IL-12 after 20h and without IL-12 in serum free conditions at 48h. This was reduced by blocking sortilin with neurotensin. However, we found no evidence that NK cells produce endogenous proNGF or NGF. We conclude that proNGF induced apoptosis of NK cells may have important implications for limiting the innate immune response.

show abstract

Functional monoclonal antibodies to p75 neurotrophin receptor raised in knockout mice

Cited by 30 publications

References 45 publications

Chronic Exposure to Nerve Growth Factor Increases Acetylcholine and Glutamate Release from Cholinergic Neurons of the Rat Medial Septum and Diagonal Band of Broca via Mechanisms Mediated by p75^NTR

Chronic Exposure to Nerve Growth Factor Increases Acetylcholine and Glutamate Release from Cholinergic Neurons of the Rat Medial Septum and Diagonal Band of Broca via Mechanisms Mediated by p75^NTR

Targeted drug delivery using genetically engineered diatom biosilica

ProNGF mediates death of Natural Killer cells through activation of the p75NTR–sortilin complex

Contact Info

Product

Resources

About

Functional monoclonal antibodies to p75 neurotrophin receptor raised in knockout mice

Cited by 30 publications

References 45 publications

Chronic Exposure to Nerve Growth Factor Increases Acetylcholine and Glutamate Release from Cholinergic Neurons of the Rat Medial Septum and Diagonal Band of Broca via Mechanisms Mediated by p75NTR

Chronic Exposure to Nerve Growth Factor Increases Acetylcholine and Glutamate Release from Cholinergic Neurons of the Rat Medial Septum and Diagonal Band of Broca via Mechanisms Mediated by p75NTR

Targeted drug delivery using genetically engineered diatom biosilica

ProNGF mediates death of Natural Killer cells through activation of the p75NTR–sortilin complex

Contact Info

Product

Resources

About

Chronic Exposure to Nerve Growth Factor Increases Acetylcholine and Glutamate Release from Cholinergic Neurons of the Rat Medial Septum and Diagonal Band of Broca via Mechanisms Mediated by p75^NTR

Chronic Exposure to Nerve Growth Factor Increases Acetylcholine and Glutamate Release from Cholinergic Neurons of the Rat Medial Septum and Diagonal Band of Broca via Mechanisms Mediated by p75^NTR