Functional nerve recovery after bridging a 15 mm gap in rat sciatic nerve with a biodegradable nerve guide

Meek, Marcel F.; Werff, J. F. A. van der; Klok, Fester; Robinson, P. H.; Nicolai, J. P. A.; Gramsbergen, Albert

doi:10.1080/02844310310019464

Cited by 32 publications

(29 citation statements)

References 33 publications

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“…On the other hand, recordings from the gastrocnemius muscle have been applied in various studies. These studies do not contain any inconsistencies about recording from gastrocnemius muscle [23][24][25] .…”

Section: Discussionmentioning

confidence: 99%

Minimally Invasive Motor Nerve Conduction Study of the Rat Sciatic and Tail Nerves

Huseyinoglu¹,

Özaydin²,

Hüseyınoğlu³

et al. 2013

Kafkas Univ Vet Fak Derg

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SummaryHuman and veterinary scientific researchers widely use rats as experimental animals. The rat nerves conduction studies are used for investigations of neural tissue injury, neural regeneration, peripheral neuropathies etc. in experimental models. Nerve conduction studies on animals do not follow strict rules, as studies in humans. Various methods and lack of the single recognized technique of nerve conduction study on the animals often lead to difficulties in the interpretation and comparison of the results of similar studies. In the present study, we have described the minimally invasive motor nerve conduction study on the rat sciatic and tail nerves. Electrophysiological examination including nerve conduction velocity and compound muscle action potential amplitudes measurements were performed on six normal growing male adult Wistar Albino rats. The mean motor nerve conduction velocity of the sciatic nerves was 58.90±5.07 m/s and of the tail nerves was 40.23±2.39 m/s. The mean compound muscle action potential amplitudes of the sciatic and tail nerves were 17.91±6.75 mV and 1.89±0.49 mV, respectively. Similar results of previous in vitro and in vivo studies prove the objectivity and reliability of our nerve conduction technique by bipolar needle electrodes on the non-exposed nerves. Keywords: Nerve conduction study, Sciatic nerve, Tail nerves, Rat Rat Siyatik ve Kuyruk Motor Sinirlerinin Minimal İnvaziv İletim Çalışması ÖzetBeşeri ve veteriner bilimsel araştırmacılar, ratları deneysel hayvan olarak yaygınca kullanmaktadırlar. Rat sinir iletim çalışmaları sinir dokusu hasarı, sinir rejenerasyonu, periferik nöropati ve buna benzer deneysel araştırma modellerinde kullanılmaktadır. Hayvanlar üzerinde yapılan sinir iletim çalışmaları, insan çalışmalarından farklı olarak, katı kurallara uymaz. Hayvanlarda sinir iletim çalışmalarının çeşitli yöntemleri ve tek tanınan tekniğin olmayışı benzer çalışmaların sonuçlarının yorumlanmasında ve kıyaslanmasında zorluklara yol açar. Çalışmamızın amacı, rat siyatik ve kuyruk motor sinirlerinin minimal girişimsel iletim çalışma yöntemlerini tanımlamaktır. Altı erkek erişkin Wistar Albino ratın sinir iletim hızları ile bileşik kas aksiyon potansiyelleri ölçümlerini içeren elektrofizyolojik incelemesi yapıldı. Ortalama motor sinir iletim hızları siyatik sinirlerde 58.90±5.07 m/sn ve kuyruk sinirlerinde 40.23±2.39 m/sn idi. Siyatik ve kuyruk sinirlerin ortalama bileşik kas aksiyon potansiyellerinin genliği sırasıyla 17.91±6.75 mV ve 1.89±0.49 mV idi. Bipolar iğne elektrotlar ile ve sinir ekspozisyonu yapılmadan uyguladığımız bu sinir iletim tekniğimizin sonuçlarının daha önceki in vitro ve in vivo çalışmaların sonuçları ile benzerlik göstermesi bu tekniğinin objektiflik ve güvenilirliğini desteklemektedir.

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Section: Discussionmentioning

confidence: 99%

Minimally Invasive Motor Nerve Conduction Study of the Rat Sciatic and Tail Nerves

Huseyinoglu¹,

Özaydin²,

Hüseyınoğlu³

et al. 2013

Kafkas Univ Vet Fak Derg

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“…15 This suggests that the factors which guided the outgrowing motor axons towards their target muscles at initial stages of development (e.g. reorganizations of neural circuits at spinal levels or compensations or 'relearning' at central levels) are no longer effective at this age in rats.…”

Section: Discussionmentioning

confidence: 99%

Functional recovery after transection of the sciatic nerve at an early age: a pilot study in rats

Meek¹,

Ijkema‐Paassen²,

Gramsbergen³

2007

Develop Med Child Neuro

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It is often clinically noted that peripheral nerve transections in infants and very young children seem to show better functional recovery after nerve repair compared with adult patients. There can be several reasons for this (e.g. outgrowing axons might have maintained their potential to locate their genuine target muscle, the distance from the lesion site to the target organ is shorter, or there is enhanced compensational capacity of central brain areas at early stages of development). We decided to study the effects on walking performance of sciatic nerve transection and repair in nine rats at the youngest age at which appreciable numbers of motoneurons survive transaction (10th day of life). The follow-up period was 52 weeks. Walking movements were recorded on video and performance was qualitatively assessed. The stance factor and the sciatic function index were calculated and abnormalities such as automutilation of the toes or flexion contractures were noted. Postmortem inspection aimed to examine the continuity of the regenerated nerve and the presence of neuromas. Nine days after the transection and nerve repair, walking performance in all rats was close to the level in rats that had not undergone the procedure. Exorotation of the hind leg on the operated side was observed in only two rats. However, 20 days after lesioning the walking performance started to deteriorate, and walking patterns in all but one rat showed severe disability until the end of the observations. Only one out of the nine animals walked relatively undisturbed, with a sciatic function index approaching normal values but with an abnormal stance factor. We conclude that a sciatic nerve transection in rats aged 10 days leads to long-term impairments in walking patterns.Clinical evidence has suggested that peripheral nerve lesions or transections at neonatal and young age may lead to less severe impairments than similar lesions at adult age, 1 and comparable results have been reported in infant monkeys. 2 Others, however, have questioned this optimism. 3 There is only scant evidence from animal experiments. The effects of transection and repair of the posterior tibial nerve at 6 and 22 days in rats has been studied; 4 walking in the animals operated at the 6th day was more impaired than in rats operated at the 22nd day.Adult motoneuronal axons have the capacity to grow out towards the distal stump of a severed sciatic nerve. However, when arriving at the target area, the outgrowing axons obviously are unable to relocate their muscles of origin and they randomly reinnervate these muscles. 5 Retrograde labelling of motoneurons showed different neurons within one and the same motoneuronal pool but innervating antagonistic muscles. This, and the drastic effects on the properties of the denervated and reinnervated muscles, are the main reasons for maladaptive functioning. 6 It might be hypothesized that nerve transections at early stages of development have less severe consequences. The outgrowing axons might still have maintained their potenti...

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“…In another peripheral vascular study performed in 1994, it was concluded that muscle atrophy of the injured limb was responsible for the reduction of the stance duration. 56 The relation of stance phase duration at the operated and nonoperated sides, de®ned by the stance factor, was considered by Dijkstra et al 62 and Meek et al 68 to be a precise method for the evaluation of functional motor nerve recovery in a nerve guide model. Recently, in sciatic and tibial nerve injury models, the shortened stance phase was related to a decreased power in the injured limb.…”

Section: 64à66mentioning

confidence: 99%

Functional assessment of peripheral nerve recovery in the rat: Gait kinematics

Varejão¹,

Cabrita²,

Patrício

et al. 2001

Microsurgery

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Computerized rat gait analysis has become an invaluable technique of functional evaluation for some peripheral nerve investigators, comparing the normal and the pathological kinematic data. Appropriate selection of the methods to evaluate the functional outcome should be sensitive enough to moderate changes. By combining kinematic data and traditional methods in regeneration studies, it is possible to achieve better documentation of functional changes with the passage of time. A review of the three commonly kinematic parameters used in nerve regeneration studies, such as the calculation of sciatic function index, stance factor, and ankle angle, will provide the reader with detailed information about this accurate and consistent means of evaluating peripheral nerve function after nerve injury and repair. This study aims to review the different methods and potentialities of the rat gait kinematics as a noninvasive evaluation during regeneration, allowing for measurement of the rate of functional recovery in experimental studies.

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Functional nerve recovery after bridging a 15 mm gap in rat sciatic nerve with a biodegradable nerve guide

Cited by 32 publications

References 33 publications

Minimally Invasive Motor Nerve Conduction Study of the Rat Sciatic and Tail Nerves

Minimally Invasive Motor Nerve Conduction Study of the Rat Sciatic and Tail Nerves

Functional recovery after transection of the sciatic nerve at an early age: a pilot study in rats

Functional assessment of peripheral nerve recovery in the rat: Gait kinematics

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