Functional peptide presentation on different hydrogen bonding biomaterials using supramolecular additives

Gaal, Ronald C. van; Buskermolen, Antonetta B.C.; Ippel, Bastiaan D.; Fransen, Peter-Paul K.H.; Zaccaria, Sabrina; Bouten, Cvc Carlijn; Dankers, Patricia Y. W.

doi:10.1016/j.biomaterials.2019.119466

Cited by 19 publications

(26 citation statements)

References 59 publications

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“…The employed BU-additive design entailing C 6 -urea-C 4 -urea-C 12 -(OEG) 12 -R, where R can be a variety of functional groups, was shown to be successful in presenting functional peptides at the biomaterial surface. 29 Screening of the synthetic additive library indicated that all BU-additives were unable to exert a meaningful positive effect on monolayer quality of HK-2, with overgrowth and defects observed in the cell layer (Fig. 3A-C and S6 †).…”

Section: Synthetic Supramolecular Biomaterials Library Screening For Mmentioning

confidence: 99%

“…According to previous studies, BUpeptides additives were estimated to be effective at a concentration of 1 mol%. 17,19,29 BU-peptide ineffectiveness could be caused by insufficient peptide presentation. Nevertheless, a concentration increase from 1 mol% to ultimately 5 mol% did not improve monolayer confluency for RPTEC cells (Fig.…”

Section: Synthetic Supramolecular Biomaterials Library Screening For Mmentioning

confidence: 99%

“…19 The presently employed BUsystem has superior effective peptide presenting properties compared to the previously employed material system. 29 This suggests that any initial cues provided by the BU-peptides are unable to steer the currently selected cell types to form full coverage monolayers. Monomeric catechol surface functionalization by BU-catechol addition resulted in fully confluent RPTEC monolayers, yet not for HK-2 cells.…”

Section: Biomaterials Sciencementioning

confidence: 99%

“…1B). [25][26][27][28][29] BU-moieties allow for more efficient surface functionalization compared to UPy-units, resulting in a more pronounced cellular response. 29 In combination with the aforementioned features, the intrinsic modular nature of supramolecular BU-moieties makes them eminently suitable for the creation of complex screening libraries.…”

Section: Introductionmentioning

confidence: 99%

“…As a base material PCL-BU was selected, which has been shown to effectively present BU-peptides at the surface. 29 Initial screening was performed with two different REC cell lines, i.e. renal proximal tubule epithelial cells (RPTEC) and human kidney 2 cells (HK-2).…”

Section: Introductionmentioning

confidence: 99%

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Biomaterial screening of protein coatings and peptide additives: towards a simple synthetic mimic of a complex natural coating for a bio-artificial kidney

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Section: Synthetic Supramolecular Biomaterials Library Screening For Mmentioning

confidence: 99%

Section: Synthetic Supramolecular Biomaterials Library Screening For Mmentioning

confidence: 99%

Section: Biomaterials Sciencementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biomaterial screening of protein coatings and peptide additives: towards a simple synthetic mimic of a complex natural coating for a bio-artificial kidney

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Renal Epithelial Cell Responses to Supramolecular Thermoplastic Elastomeric Concave and Convex Structures

Gaal

Miltenburg

Kurniawan

et al. 2020

Adv Materials Inter

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The nephron naturally provides a concave conformation for epithelial cells, yet biomedical applications such as bioartificial kidney can employ convex seeding. Frequently glass or polydimethylsiloxane are utilized as base materials to study renal epithelial cell response to curvatures. Insights on relevant materials for biomedical applications remain limited. Here it is investigated how human immortalized renal proximal tubule epithelial cells (RPTEC) respond to a range of concave and convex curvatures made from a bis‐urea modified polycaprolactone material. Solvent cast chips containing a 50–500 µm diameter range of both concave and convex semicylindrical structures are successfully produced. Concave structures are completely covered by cells under all conditions. Yet cell layers present gaps on the summits of convex structures; the relative gap size enlarges as the diameter decreased. Increased proliferation time and cell seeding density allow for cells to overcome summit avoidance and nearly engulf convex structures. Interestingly, sample inversion also results in gap closure on convex structures during live imaging. Polarization of RPTECs is more prominent on concave compared to convex structures. These findings suggest that biomedical applications such as the bioartificial kidney should focus on concave seeding of cells to acquire more functional cell monolayers.

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Advances in Additive Manufactured Scaffolds Mimicking the Osteochondral Interface

Beeren,

Dijkstra,

Mota

et al. 2024

Advanced NanoBiomed Research

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Architectural, compositional, and mechanical gradients are present in many interfacial tissues in the body. Yet desired for regeneration, the recreation of these complex natural gradients in porous scaffolds remains a challenging task. Additive manufacturing (AM) has been highlighted as a technology to fabricate constructs to regenerate interfacial tissues. Integration of different types of gradients, which can be physical, mechanical, and/or biochemical, shows promise to control cell fate and the regeneration process in a spatial controlled manner. One of the most studied tissue interfaces is the osteochondral unit which connects cartilage to bone. This tissue is often damaged because of trauma or ageing, leading to osteoarthritis; a degenerative disease and a major cause of disability worldwide. Therefore, in view of osteochondral (OC) regeneration, a state‐of‐the‐art overview of current approaches is presented to manufacture gradient scaffolds prepared by AM techniques. The focus is on thermoplastic, hydrogel, and hybrid scaffolds comprising gradients that induce biomimicry by their physical and biological properties. The effect of these different systems on OC tissue formation in‐vitro and in‐vivo is addressed. Finally, an outlook on current trends of dynamic materials is provided, including proposals on how these materials could improve the mimicry of scaffolds applied for OC regeneration.

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Functional peptide presentation on different hydrogen bonding biomaterials using supramolecular additives

Cited by 19 publications

References 59 publications

Biomaterial screening of protein coatings and peptide additives: towards a simple synthetic mimic of a complex natural coating for a bio-artificial kidney

Biomaterial screening of protein coatings and peptide additives: towards a simple synthetic mimic of a complex natural coating for a bio-artificial kidney

Renal Epithelial Cell Responses to Supramolecular Thermoplastic Elastomeric Concave and Convex Structures

Advances in Additive Manufactured Scaffolds Mimicking the Osteochondral Interface

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