Functional recovery after transplantation of bone marrow-derived human mesenchymal stromal cells in a rat model of spinal cord injury

Pal, Rakhi; Gopinath, Chaitanya; Rao, Nagesh M; Banerjee, Poulomi; Krishnamoorthy, Venkatesh; Venkataramana, N. K.; Totey, S.M.

doi:10.3109/14653249.2010.487899

Cited by 58 publications

(38 citation statements)

References 29 publications

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“…BMSCs survived for more than 30 days and found to have migrated to the injury site. However, those cells were not observed to have differentiated into other cells, possibly due to the short residing duration of the cells in the lesion site [233]. In contrast to previous findings, Himes et al discovered, based on a contusion SCI rat model, that the survival of BMSCs consistently declined during the course of study and failed to detect their robust survival 11 weeks after surgery [234].…”

Section: Mesenchymal Stem Cells (Mscs)contrasting

confidence: 50%

“…Furthermore, although MSCs did not improve motor functional recovery, they induced some hindlimb stepping activity, which might be attributable to the release of a diffusible cell-activating substance [232]. In order to assess the effects of BMSC dose, transplantation site and time (during and after SCI) on SCI repair, Pal et al transplanted human BMSCs into a contusive SCI rat model [233]. They found that the cell transplantation significantly improved the motor and sensory functional recovery and larger dose of cells showed better results.…”

Section: Mesenchymal Stem Cells (Mscs)mentioning

confidence: 99%

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Biodegradable Cell-Seeded Nanofiber Scaffolds for Neural Repair

Han

Cheung

2011

Polymers

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Central and peripheral neural injuries are traumatic and can lead to loss of motor and sensory function, chronic pain, and permanent disability. Strategies that bridge the site of injury and allow axonal regeneration promise to have a large impact on restoring quality of life for these patients. Engineered materials can be used to guide axonal growth. Specifically, nanofiber structures can mimic the natural extracellular matrix, and aligned nanofibers have been shown to direct neurite outgrowth and support axon regeneration. In addition, cell-seeded scaffolds can assist in the remyelination of the regenerating axons. The electrospinning process allows control over fiber diameter, alignment, porosity, and morphology. Biodegradable polymers have been electrospun and their use in tissue engineering has been demonstrated. This paper discusses aspects of electrospun biodegradable nanofibers for neural regeneration, how fiber alignment affects cell alignment, and how cell-seeded scaffolds can increase the effectiveness of such implants.

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Section: Mesenchymal Stem Cells (Mscs)contrasting

confidence: 50%

Section: Mesenchymal Stem Cells (Mscs)mentioning

confidence: 99%

Biodegradable Cell-Seeded Nanofiber Scaffolds for Neural Repair

Han

Cheung

2011

Polymers

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“…First, murine BMSCs, unlike their human equivalent, lack major histocompatibility complex (MHC) class II expression [13], and second, T-cell inhibition by BMSCs requires cell contact in mice but is mediated by soluble factors in humans [14,15]. In any event, reports of successful xenotransplantation with BMSCs from various species continue to appear frequently; for example, rat BMSC for bone formation in rabbit [16], human BMSC for spinal cord injury in rat [17,18], and human BMSC for bone formation in mice [19].…”

Section: Stem Cells and Developmentmentioning

confidence: 99%

Allogeneic and Xenogeneic Transplantation of Adipose-Derived Stem Cells in Immunocompetent Recipients Without Immunosuppressants

Lin

Lue

2012

Stem Cells and Development

187

152

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“…Several studies have been reported on xenogenic MSCs including studies in dogs by Plotnikov et al (2007), nude rats by Guest et al (2008), Wistar rats by Pal et al (2010), Park et al (2010), and Villanova Junior (2013), Balb/c mice by Ra et al (2011), who reported no adverse effects after transplantation and Source: Elaboration of the authors.…”

Section: In Vivo Evaluation Of Transplanted Hadscmentioning

confidence: 99%

“…The use of human stem cells in rats with spinal cord injury revealed migration of cells to the injured site (PAL et al, 2010) and reduction of the inflammatory reaction (PARK et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Effect of mesenchymal stem cells on movement and urination of rats with spinal cord injury

et al. 2014

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Cell therapy has frequently been reported as a possible treatment for spinal trauma in humans and animals; however, without pharmacologically curative action on damage from the primary lesion. In this study, we evaluated the effect of administering human adipose-derived stem cells (hADSC) in rats after spinal cord injury. The hADSC were used between the third and fifth passages and a proportion of cells were transduced for screening in vivo after transplantation. Spinal cord injury was induced with a Fogarty catheter no. 3 inserted into the epidural space with a cuff located at T8 and filled with 80-μL saline for 5 min. The control group A (n = 12) received culture medium (50 μL) and group B (n = 12) received hADSC (1.2 × 10 6 ) at 7 and 14 days post-injury, in the tail vein. Emptying of the bladder by massage was performed daily for 3 months. Evaluation of functional motor activity was performed daily until 3 months post-injury using the Basso-Beattie-Bresnahan scale. Subsequently, the animals were euthanized and histological analysis of the urinary bladder and spinal cord was performed. Bioluminescence analysis revealed hADSC at the application site and lungs. There was improvement of urinary bladder function in 83.3% animals in group B and 16.66% animals in group A. The analysis of functional motor activity and histology of the spinal cord and urinary bladder demonstrated no significant difference between groups A and B. The results indicate that transplanted hADSC improved urinary function via a telecrine mechanism, namely action at a distance. Key words: Spinal cord injury, paraplegia, urinary incontinence, cell therapy, bioluminescence ResumoA terapia celular tem sido reportada como possibilidade de tratamento para os traumas medulares, frequentes em pessoas e em animais, e que não apresentam cura farmacológica para danos advindos da lesão primária. Foram avaliados os efeitos da aplicação de células-tronco derivadas do tecido adiposo humano (hADSC) em ratos Wistar após lesão medular. As hADSC foram cultivadas, utilizadas entre a terceira e quinta passagens e parte delas foi transduzida para o rastreamento in vivo após o transplante. A lesão medular foi realizada com cateter de Fogarty nº. 3, introduzido no espaço epidural, com cuff localizado em T8 e preenchido com 80 μL de solução salina, por 5 minutos. Os animais do grupo controle (A, n=12) receberam aplicações de meio de cultura (50 μL) e o grupo B (n=12) recebeu duas aplicações de hADSC (1,2 x 10 6 ), sete e 14 dias pós-lesão, ambas na veia da cauda. O esvaziamento vesical, por massagem, foi efetuado diariamente, durante três meses. As avaliações motoras foram repetidas, diariamente, até três meses pós-lesão, utilizando a escala Basso-Beattie-Bresneham. Após este período os animais foram submetidos à eutanásia e foram realizadas análises histológicas de bexiga urinária e medula espinhal. As análises de bioluminescência revelaram hADSC no local da aplicação e nos pulmões. Houve melhora da função da bexiga urinária em 83,3% dos animais do grupo B...

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Functional recovery after transplantation of bone marrow-derived human mesenchymal stromal cells in a rat model of spinal cord injury

Cited by 58 publications

References 29 publications

Biodegradable Cell-Seeded Nanofiber Scaffolds for Neural Repair

Biodegradable Cell-Seeded Nanofiber Scaffolds for Neural Repair

Allogeneic and Xenogeneic Transplantation of Adipose-Derived Stem Cells in Immunocompetent Recipients Without Immunosuppressants

Effect of mesenchymal stem cells on movement and urination of rats with spinal cord injury

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