“…As expected, based on cohesin interactions with Mediator and with CTCF (Kagey et al, 2010; Xiao et al, 2011), MED1 and cohesin loading factor NIPBL can immunoprecipitate CTCF and cohesin component SMC1 (Figure 3A). Similarly, antibodies to GATA1 immunoprecipitate CTCF and MED1 (Stumpf et al, 2006; Manavathi et al, 2012) and SMC1, possibly indirectly (Xiao et al, 2011). Consistent with GATA1 principally functioning as part of the LDB1 complex (Li et al, 2013), LDB1 antibodies also immunoprecipitate CTCF and SMC1, although not MED1.…”