Functional role of a specialized class of spinal commissural inhibitory neurons during fast escapes in zebrafish

Satou, Chie; Kimura, Yukiko; Kohashi, Tsunehiko; Horikawa, Kazuki; Takeda, Hiroyuki; Oda, Yoichi; Higashijima, Shin-ichi

doi:10.1016/j.neures.2009.09.469

Cited by 38 publications

(86 citation statements)

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“…Experiments were carried out at room temperature (25-28°C). Wild type and five of transgenic lines were used: glyt2:GFP (McLean et al 2007), Tol-056 (expressing GFP in the M-cells; Satou et al 2009;Tanimoto et al 2009), hspGFF62A (Asakawa et al 2008), UAS: mCherry-2A-AIP2 (this study) and UAS:mCherry-2A-ACP2 (this study). From 26 hpf, the hspGFF62A fish expresses Gal4FF (Asakawa et al 2008) in the M-cells, other neurons and cardiac muscles.…”

Section: Methodsmentioning

confidence: 99%

Glycinergic transmission and postsynaptic activation of CaMKII are required for glycine receptor clustering in vivo

et al. 2013

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Synaptic transmission‐dependent regulation of neurotransmitter receptor accumulation at postsynaptic sites underlies the formation, maintenance and maturation of synaptic function. Previous in vitro studies showed that glycine receptor (GlyR) clustering requires synaptic inputs. However, in vivo GlyR regulation by synaptic transmission is not fully understood. Here, we established a model system using developing zebrafish, in which GlyRs are expressed in Mauthner cells (M‐cells), a pair of giant, reticulospinal, hindbrain neurons, thereby enabling analysis of GlyR clusters over time in identifiable cells. Bath application of a glycinergic blocker, strychnine, to developing zebrafish prevented postsynaptic GlyR cluster formation in the M‐cells. After strychnine removal, the GlyR clusters appeared in the M‐cells. At a later stage, glycinergic transmission blockade impaired maintenance of GlyR clusters. We also found that pharmacological blockade of either L‐type Ca2+ channels or calcium‐/calmodulin‐dependent protein kinase II (CaMKII) disturbed GlyR clustering. In addition, the M‐cell‐specific CaMKII inactivation using the Gal4‐UAS system significantly impaired GlyR clustering in the M‐cells. Thus, the formation and maintenance of GlyR clusters in the M‐cells in the developing animals are regulated in a synaptic transmission‐dependent manner, and CaMKII activation at the postsynapse is essential for GlyR clustering. This is the first demonstration of synaptic transmission‐dependent modulation of synaptic GlyRs in vivo.

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Section: Methodsmentioning

confidence: 99%

Glycinergic transmission and postsynaptic activation of CaMKII are required for glycine receptor clustering in vivo

et al. 2013

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“…Further work is required to identify neurons that contain Cx35 immunoreactivity. Satou et al (2009) examined Cx35 immunoreactivity in the larval spinal cord. Widely distributed immunoreactive puncta were found throughout the spinal cord, but the most intensely labeled sites corresponded to appositions of the Mauthner axons to CoLo inhibitory interneurons (Satou et al, 2009).…”

Section: Cx35b Expression During Spinal Cord Developmentmentioning

confidence: 99%

Connexin 35b expression in the spinal cord of Danio rerio embryos and larvae

Carlisle

Ribera

2014

J of Comparative Neurology

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Electrical synapses are expressed prominently in the developing and mature nervous systems. Unlike chemical synapses, little is known about the developmental role of electrical synapses, reflecting the limitations imposed by the lack of selective pharmacological blockers. At a molecular level, the building blocks of electrical synapses are connexin proteins. In this study, we report the expression pattern for neuronally expressed connexin 35b (cx35b), the zebrafish orthologue of mammalian Connexin (Cx) 36. We find that cx35b is expressed at the time of neural induction, indicating a possible early role in neural progenitor cells. Additionally, cx35b localizes to the ventral spinal cord during embryonic and early larval stages. We detect cx35b mRNA in secondary motor neurons (SMNs) and interneurons. We identified the premotor circumferential descending (CiD) interneuron as one interneuron subtype expressing cx35b. In addition, cx35b is present in other ventral interneurons of unknown subtype(s). This early expression of cx35b in SMNs and CiDs suggests a possible role in motor network function during embryonic and larval stages.

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“…9D). To observe the axons of another type of commissural neuron, we crossed SAGFF(LF)134A;Tg(UAS:RFP) with the Tol-056 enhancer trap line, which expresses GFP in Mauthner cells, commissural local interneurons (CoLo), and Kolmer-Agduhr (KA) neurons (Satou et al 2009). In SAG-FF(LF)134A;Tg(UAS:RFP);Tol-056 at 3 dpf, we could trace each commissural axon from CoLo neurons by using the series of Z-stack images.…”

Section: Location Of Paths For Axons Of Commissural Neurons In the Flmentioning

confidence: 99%

“…CoLos are glycinergic interneurons, present at one cell per hemi-segment, active only during escape behaviors. They are activated by the ipsilateral Mauthner-axon, and shut down the activity of contralateral motoneurons (Liao & Fetcho 2008;Satou et al 2009). The CoLo axon extends ventrally and, after encountering the ipsilateral Mauthner axon on its dorsal side, crosses the midline of the spinal cord by a route dorsal to the Mauthner axon (Satou et al 2009).…”

Section: Midline Glial Structures In the Spinal Cordmentioning

confidence: 99%

“…They are activated by the ipsilateral Mauthner-axon, and shut down the activity of contralateral motoneurons (Liao & Fetcho 2008;Satou et al 2009). The CoLo axon extends ventrally and, after encountering the ipsilateral Mauthner axon on its dorsal side, crosses the midline of the spinal cord by a route dorsal to the Mauthner axon (Satou et al 2009). It is also possible that CoLo axons penetrate the endfoot of the floor-plate cell or on the basal membrane at early stages, and that their paths in the floor plate shift dorsally.…”

Section: Midline Glial Structures In the Spinal Cordmentioning

confidence: 99%

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Transgenic line with gal4 insertion useful to study morphogenesis of craniofacial perichondrium, vascular endothelium‐associated cells, floor plate, and dorsal midline radial glia during zebrafish development

et al. 2012

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Zebrafish is a good model for studying vertebrate development because of the availability of powerful genetic tools. We are interested in the study of the craniofacial skeletal structure of the zebrafish. For this purpose, we performed a gene trap screen and identified a Gal4 gene trap line, SAGFF(LF)134A. We then analyzed the expression pattern of SAGFF(LF)134A;Tg(UAS:GFP) and found that GFP was expressed not only in craniofacial skeletal elements but also in the vascular system, as well as in the nervous system. In craniofacial skeletal elements, strong GFP expression was detected not only in chondrocytes but also in the perichondrium. In the vascular system, GFP was expressed in endothelium-associated cells. In the spinal cord, strong GFP expression was found in the floor plate, and later in the dorsal radial glia located on the midline. Taking advantage of this transgenic line, which drives Gal4 expression in specific tissues, we crossed SAGFF(LF)134A with several UAS reporter lines. In particular, time-lapse imaging of photoconverted floor-plate cells of SAGFF(LF)134A;Tg(UAS:KikGR) revealed that the floor-plate cells changed their shape within 36 hours from cuboidal/trapezoidal to wine glass shaped. Moreover, we identified a novel mode of association between axons and glia. The putative paths for the commissural axons, including pax8-positive CoBL interneurons, were identified as small openings in the basal endfoot of each floor plate. Our results indicate that the transgenic line would be useful for studying the morphogenesis of less-well-characterized tissues of interest, including the perichondrium, dorsal midline radial glia, late-stage floor plate, and vascular endothelium-associated cells.

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Functional role of a specialized class of spinal commissural inhibitory neurons during fast escapes in zebrafish

Cited by 38 publications

References 0 publications

Glycinergic transmission and postsynaptic activation of CaMKII are required for glycine receptor clustering in vivo

Glycinergic transmission and postsynaptic activation of CaMKII are required for glycine receptor clustering in vivo

Connexin 35b expression in the spinal cord of Danio rerio embryos and larvae

Transgenic line with gal4 insertion useful to study morphogenesis of craniofacial perichondrium, vascular endothelium‐associated cells, floor plate, and dorsal midline radial glia during zebrafish development

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