Functional role of LASP1 in cell viability and its regulation by microRNAs in bladder cancer

Chiyomaru, Takeshi; Enokida, Hideki; Kawakami, Kazumori; Tatarano, Shuichi; Uchida, Youhei; Kawahara, Kazuya; Nishiyama, Kenryu; Seki, Naohiko; Nakagawa, Masayuki

doi:10.1016/j.urolonc.2010.05.008

Cited by 101 publications

(100 citation statements)

References 23 publications

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“…The initial gap length (0 h) and residual gap length 24 h after wounding were calculated from photomicrographs as described previously. 15,16 A cell invasion assay was carried out using modified Boyden Chambers consisting of transwell-precoated Matrigel membrane filter inserts with 8 mm pores in 24-well tissue culture plates (BD Biosciences, Bedford, MA, USA). Minimum essential medium containing 10% fetal bovine serum in the lower chamber served as the chemoattractant, as described previously.…”

Section: Cell Proliferation Migration and Invasion Assaysmentioning

confidence: 99%

“…Minimum essential medium containing 10% fetal bovine serum in the lower chamber served as the chemoattractant, as described previously. 15,16 All experiments were performed in triplicate.…”

Section: Cell Proliferation Migration and Invasion Assaysmentioning

confidence: 99%

“…Oligo-microarray human 44 K (Agilent) was used for gene expression analysis, and procedure of the experiment as described previously. 15,16 To identify the biological processes or pathways potentially regulated by selected miRNAs, we performed GeneCodis analysis (http://genecodis. dacya.ucm.es/) with our predicted target list.…”

Section: Identification Of Mir-222 or Mir-31 Regulated Target Genes Amentioning

confidence: 99%

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Tumor suppressive microRNAs (miR-222 and miR-31) regulate molecular pathways based on microRNA expression signature in prostate cancer

et al. 2012

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microRNAs (miRNAs) have key roles in human tumorigenesis, tumor progression and metastasis. miRNAs are aberrantly expressed in many human cancers and can function as tumor suppressors or oncogenes that target many cancer-related genes. This study seeks to identify novel miRNA-regulated molecular pathways in prostate cancer (PCa). The miRNA expression signature in clinical specimens of PCa showed that 56 miRNAs were significantly downregulated in PCa compared with nonPCa tissues. We focused on the top four downregulated miRNAs (miR-187, miR-205, miR-222 and miR-31) to investigate their functional significance in PCa cells. Expression levels of these four miRNAs were validated in PCa specimens (15 PCa tissues and 17 non-PCa tissues) to confirm that they were significantly reduced in these PCa tissues. Gain-of-function analysis demonstrated that miR-222 and miR-31 inhibited cell proliferation, invasion and migration in PCa cell lines (PC3 and DU145), suggesting that miR-222 and miR-31 may act as tumor suppressors in PCa. Genome-wide gene expression analysis using miR-222 or miR-31 transfectants to identify the pathways they affect showed that many cancer-related genes are regulated by these miRNAs in PC3 cells. Identification and categorization of the molecular pathways regulated by tumor suppressive miRNAs could provide new information about the molecular mechanisms of PCa tumorigenesis.

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Section: Cell Proliferation Migration and Invasion Assaysmentioning

confidence: 99%

“…Minimum essential medium containing 10% fetal bovine serum in the lower chamber served as the chemoattractant, as described previously. 15,16 All experiments were performed in triplicate.…”

Section: Cell Proliferation Migration and Invasion Assaysmentioning

confidence: 99%

Section: Identification Of Mir-222 or Mir-31 Regulated Target Genes Amentioning

confidence: 99%

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Tumor suppressive microRNAs (miR-222 and miR-31) regulate molecular pathways based on microRNA expression signature in prostate cancer

et al. 2012

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“…Recent studies have demonstrated that miR-218 is downregulated in various types of cancer, including cervical cancer, gastric cancer, lung cancer, colon cancer, prostate cancer, and bladder cancer (Davidson et al, 2010;Song et al, 2010;Tie et al, 2010;Leite et al, 2011;Uesugi et al, 2011;Li et al, 2012a;Li et al, 2012b;Chiyomaru et al, 2012;He et al, 2012). However, the level of miR0218 expression, and its clinical significance in pancreatic cancer, remains to be investigated.…”

Section: Discussionmentioning

confidence: 99%

“…microRNA-218 (miR-218) is a vertebrate-specific miRNA that has been predicted and experimentally confirmed to play a crucial role in tumorigenesis and tumor progression by regulating the expression of potential targets (Hassan et al, 2012). Recent studies have indicated that miR-218 is down-regulated in various types of cancer, including cervical cancer, gastric cancer, lung cancer, colon cancer, prostate cancer, and bladder cancer (Davidson et al, 2010;Song et al, 2010;Tie et al, 2010;Uesugi et al, 2011;Leite et al, 2011;Li et al, 2012a,b;Chiyomaru et al, 2012;He et al, 2012). However, the level of expression of miR-218 and its clinical significance in pancreatic cancer has not been investigated.…”

Section: Introductionmentioning

confidence: 99%

Reduced miRNA-218 expression in pancreatic cancer patients as a predictor of poor prognosis

2015

Genet. Mol. Res.

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ABSTRACT. microRNA-218 (miR-218) is a vertebrate-specific miRNA that plays a crucial role in tumorigenesis and tumor progression. This study analyzed the miR-218 expression level and clinical significance in pancreatic cancer. One hundred and seven pairs of pancreatic cancer and adjacent normal tissues were analyzed by quantitative reversetranscriptase polymerase chain reaction. The correlation between miR-218 expression and clinicopathological characters was determined by the two-sample Student t-test. The survival correlations were analyzed by the Kaplan-Meier method and Cox proportional hazards model. The relative expression of miR-218 in pancreatic cancer tissues (2.63 ± 1.59) was significantly lower than that in matched noncancerous pancreatic tissues (6.52 ± 2.50, P < 0.001). The low expression of miR-218 in the pancreatic cancer tissues were strongly correlated with the TNM classification (P = 0.02), distant metastasis (P = 0.001), and tumor differentiation (P = 0.003). The low level of miR-218 expression was significantly correlated with the shorter overall survival time of pancreatic cancer patients (5-year overall survival rate: 7.5 vs 34.9%; log-rank test: P < 0.001). Multivariate analyses confirmed that a low level of miR-218 expression was an independent 16373 miR-218 is a prognosis factor for pancreatic cancer ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 16372-16378 (2015) predictor of poor prognosis in pancreatic cancer patients (Hazard ratio: 7.24; 95% confidence interval: 2.01-18.28; P = 0.007). Our findings suggested a significant downregulation in the expression of miR-218; this might have considerable potential value in the prognosis for pancreatic cancer.

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microRNA expression profile in a large series of bladder tumors: Identification of a 3‐miRNA signature associated with aggressiveness of muscle‐invasive bladder cancer

Pignot

Cizeron-Clairac

Vacher

et al. 2013

Intl Journal of Cancer

161

108

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The aim of this study was to evaluate the expression levels of microRNAs (miRNAs) in bladder tumors in order to identify miRNAs involved in bladder carcinogenesis with potential prognostic implications. Expression levels of miRNAs were assessed by quantitative real-time RT-PCR in 11 human normal bladder and 166 bladder tumor samples (86 non-muscle-invasive bladder cancer (NMIBC) and 80 muscle-invasive bladder cancer (MIBC)). The expression level of 804 miRNAs was initially measured in a well-defined series of seven NMIBC, MIBC and normal bladder samples (screening set). The most strongly deregulated miRNAs in tumor samples compared to normal bladder tissue were then selected for RT-PCR validation in a well-characterized independent series of 152 bladder tumors (validation set), and in six bladder cancer cell lines. Expression levels of these miRNAs were tested for their association with clinical outcome. A robust group of 15 miRNAs was found to be significantly deregulated in bladder cancer. Except for two miRNAs, miR-146b and miR-9, which were specifically upregulated in MIBC, the majority of miRNAs (n 5 13) were deregulated in the same way in the two types of bladder tumors, irrespective of pathological stage : three miRNAs were upregulated (miR-200b, miR-182 and miR-138) and the other 10 miRNAs were downregulated (miR-1, miR-133a, miR-133b, miR-145, miR-143, miR-204, miR-921, miR-1281, miR-199a and miR-199b). A 3-miRNA signature (miR-9, miR-182 and miR-200b) was found to be related to MIBC tumor aggressiveness and was associated with both recurrence-free and overall survival in univariate analysis with a trend to significance in the multivariate analysis (p 5 0.05). Our results suggested a promising individual prognostic value of these new markers.

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Functional role of LASP1 in cell viability and its regulation by microRNAs in bladder cancer

Cited by 101 publications

References 23 publications

Tumor suppressive microRNAs (miR-222 and miR-31) regulate molecular pathways based on microRNA expression signature in prostate cancer

Tumor suppressive microRNAs (miR-222 and miR-31) regulate molecular pathways based on microRNA expression signature in prostate cancer

Reduced miRNA-218 expression in pancreatic cancer patients as a predictor of poor prognosis

microRNA expression profile in a large series of bladder tumors: Identification of a 3‐miRNA signature associated with aggressiveness of muscle‐invasive bladder cancer

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