Functional role of lncRNA LOC101927497 in N -methyl- N ′-nitro- N -nitrosoguanidine-induced malignantly transformed human gastric epithelial cells

Luo, Yuanwei; Liang, Min; Yao, Wenqian; Liu, Jifang; Niu, Qiuling; Chen, Jitao; Liu, Zhaoyu; Li, Ming; Shi, Boyun; Pan, Jinhui; Zhou, Lin; Zhou, Xinke

doi:10.1016/j.lfs.2017.12.007

Cited by 22 publications

(21 citation statements)

References 29 publications

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“…Downregulation of AC002454.1 expression can inhibit the proliferation of leukemia cells, affect the cell cycle, increase apoptosis rate and affect the expression of CDK6 (Cao et al 2016). Other work has shown that ENST00000424523 is expressed at low levels in gastric cancer cells and inhibits both cell proliferation and migration (Luo et al 2018). However, the role of ENST00000452050 has not been reported previously in the literature.…”

Section: Discussionmentioning

confidence: 95%

AC002454.1 and CDK6 synergistically promote endometrial cell migration and invasion in endometriosis

Liu¹,

Wang²,

Chen³

et al. 2019

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Previous lncRNA microarray screening found that the AC002454.1 gene was highly expressed in endometriosis (EMS), and these expression levels were highly correlated with cyclin-dependent kinase-6 (CDK6). This study investigated the expression level and correlation between AC002454.1 and CDK6 in endometrium tissues and the influence of these changes in expression upon the biological behavior of eutopic endometrial cells. We confirmed AC002454.1 and CDK6 mRNA and protein were highly expressed in ectopic and eutopic endometrial tissue from patients with EMS and were clearly correlated. In vitro, both AC002454.1 and CDK6 positively regulated the proliferation, migration and invasion ability of eutopic endometrial cells and could promote the transformation of cells from G0/G1 phase to S phase. AC002454.1 and CDK6 may have synergistic effects, thereby affecting the biological behavior of endometrial cells, and thus promote the progression of EMS.

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Section: Discussionmentioning

confidence: 95%

AC002454.1 and CDK6 synergistically promote endometrial cell migration and invasion in endometriosis

Liu¹,

Wang²,

Chen³

et al. 2019

Reproduction

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“…High-throughput sequencing was performed and data was analyzed as we previously described (12,20,21). CircRNAs were subjected to further analysis including annotation, sequence prediction, and the calculated expression levels.…”

Section: High-throughput Sequencing and Data Analysismentioning

confidence: 99%

“…Flow cytometric analysis was performed as previously described (12). Cell cycle and apoptosis were determined on a FACScan ow cytometer (Becton Dickinson, Franklin Lakes, NJ, USA).…”

Section: Flow Cytometric Analysismentioning

confidence: 99%

“…Increasing studies have revealed the role of ncRNAs in carcinogenesis induced by environmental carcinogens, and demonstrated that alterations in ncRNAs expression are associated with exposure to chemical carcinogens (9,10). In the study by Yang et al, malignant-transformed human gastric epithelial cells (GES-1-T cells) induced by MNNG were established to explore the molecular mechanism of MNNG-induced gastric carcinogenic processes in humans (11); moreover, Yang et al and we respectively showed that microRNA (miRNA) miR-21 and long noncoding RNA LOC101927497 were aberrantly expressed and involved in malignant progression of MNNG-induced GC (11,12).…”

Section: Introductionmentioning

confidence: 99%

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Circular RNA hsa_circ_0001829 Promotes Gastric Cancer Progression through miR-155-5p/SMAD2 Axis

Niu

Dong

Liang

et al. 2020

Preprint

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Background: Accumulating evidences have shown that circular RNAs (circRNAs) play important roles in regulating the pathogenesis of cancer. However, the role of circRNAs in gastric cancer (GC) remains largely unclear. Methods: In this study, we identified a novel upregulated circRNA, hsa_circ_0001829, in chemically induced malignant transformed human gastric epithelial cells using RNA-seq. Subsequent qRT-PCR was performed to detect the expression level of hsa_circ_0001829 in GC cell lines and tissues. Functional roles of hsa_circ_0001829 in GC were then explored by loss- and gain-of- function assays. Bioinformatic prediction and luciferase assay were used to investigate potential mechanisms of hsa_circ_0001829. Finally, the mice xenograft models were constructed to assess the function of hsa_circ_0001829 in vivo.Results: We found that hsa_circ_0001829 was significantly upregulated in GC tissues and cell lines. Loss- and gain-of- function assays showed that hsa_circ_0001829 promotes GC cells proliferation, migration and invasion, and the affected cell cycle progression and apoptosis rates may account for the effect of hsa_circ_0001829 on GC proliferation. In addition, bioinformatic prediction and luciferase assay showed that hsa_circ_0001829 acts as a molecular sponge for miR-155-5p and that SMAD2 was a target gene of miR-155-5p; moreover, hsa_circ_0001829 sponges miR-155-5p to regulate SMAD2 expression and hsa_circ_0001829 promotes GC progression through the miR-155-5p–SMAD2 pathway. Finally, suppression of hsa_circ_0001829 expression inhibited subcutaneous tumor growth in vivo. Conclusion: Taken together, our findings firstly demonstrated a novel oncogenic role of hsa_circ_0001829 in GC progression, which is through miR-155-5p–SMAD2 axis, and our study may offer novel biomarkers and therapeutic targets for GC.

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“…As a result, cancer and its complications are major mental and economic burdens for patients and their families. To date, the etiology and pathogenesis of cancer have been extensively explored, but the underlying mechanisms remain unclear [ 6 , 7 ]. Prior studies indicate that environmental pollution [ 8 ], unhealthy living habits [ 9 ], viral infection [ 10 ], and radioactive exposure [ 11 ] may contribute to tumorigenesis.…”

Section: Introductionmentioning

confidence: 99%

The association between rs16917496 T/C polymorphism ofSET8gene and cancer risk in Asian populations: a meta-analysis

et al. 2018

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Epidemiological studies have demonstrated close associations between SET8 rs16917496 T/C polymorphism and cancer risk, but the results of published studies were not consistent. We therefore performed this meta-analysis to explore the associations between rs16917496 T/C polymorphism and cancer risk. Five online databases were searched. Odds ratios (ORs) with a 95% confidence interval (CI) were calculated to assess the association between rs16917496 T/C polymorphism and cancer risk. In addition, heterogeneity, accumulative, sensitivity analysis, and publication bias were conducted to check the statistical power. Overall, 13 publications involving 5878 subjects were identified according to included criteria. No significant cancer risk was observed in genetic model of SET8 rs16917496 T/C polymorphism in Asian populations (C vs. T: OR = 1.04, 95%CI = 0.88–1.23, P = 0.63%; TC vs. TT: OR = 1.17, 95%CI = 0.96–1.24, P = 0.11%; CC vs. TT: OR = 0.90, 95%CI = 0.60–1.37, P = 0.63; TC+CC vs. TT: OR = 1.11, 95%CI = 0.90–1.38, P = 0.33; CC vs. TT+TC: OR = 0.92, 95%CI = 0.65–1.30, P = 0.63). Furthermore, similar associations were found in the subgroup analysis of race diversity, control design, genotyping methods, and different cancer types. In summary, our meta-analysis indicated that the SET8 rs16917496 T/C polymorphism may not play a critical role in cancer development in Asian populations.

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Functional role of lncRNA LOC101927497 in N -methyl- N ′-nitro- N -nitrosoguanidine-induced malignantly transformed human gastric epithelial cells

Cited by 22 publications

References 29 publications

AC002454.1 and CDK6 synergistically promote endometrial cell migration and invasion in endometriosis

AC002454.1 and CDK6 synergistically promote endometrial cell migration and invasion in endometriosis

Circular RNA hsa_circ_0001829 Promotes Gastric Cancer Progression through miR-155-5p/SMAD2 Axis

The association between rs16917496 T/C polymorphism ofSET8gene and cancer risk in Asian populations: a meta-analysis

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Functional role of lncRNA LOC101927497 in N -methyl- N ′-nitro- N -nitrosoguanidine-induced malignantly transformed human gastric epithelial cells

Cited by 22 publications

References 29 publications

AC002454.1 and CDK6 synergistically promote endometrial cell migration and invasion in endometriosis

AC002454.1 and CDK6 synergistically promote endometrial cell migration and invasion in endometriosis

Circular RNA hsa_circ_0001829 Promotes&nbsp;Gastric Cancer Progression through miR-155-5p/SMAD2 Axis

The association between rs16917496 T/C polymorphism ofSET8gene and cancer risk in Asian populations: a meta-analysis

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Circular RNA hsa_circ_0001829 Promotes Gastric Cancer Progression through miR-155-5p/SMAD2 Axis