Functional role of the Tau protein in epithelial ovarian cancer cells

Yamauchi, A; Kobayashi, Asami; Oikiri, Hiroe; Yokoyama, Yoshihito

doi:10.1002/rmb2.12019

Cited by 15 publications

(18 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many regulatory factors in polymerization and dynamic behavior of microtubules include, but are not limited to, the interactions with microtubule-associated proteins, anticancer agents, or even interactions with some molecular motors [9] , [10] , [11] , [12] , [13] . In addition, the results of our studies and others indicate that the differences which exist in the composition of microtubules in terms of distribution of beta tubulin isotypes, may also alter both their biomechanical specifications, as well as functions of some molecular motors along them [25] , [26] , [27] , [28] , [29] , [30] , [31] , [32] , [33] , [34] , [35] .…”

Section: Discussionmentioning

confidence: 99%

“…Since a possible link has been reported between the overexpression of Tau protein and drug resistance, the study of the Tau on cancer microtubules will contribute to new knowledge that may be significant in overcoming drug resistance, leading to impactful therapeutic strategies. Previous studies have found that the Tau protein interacts with the beta tubulin in the microtubules’ structure [13] . Remarkably, there are multiple different beta tubulin isotypes.…”

Section: Introductionmentioning

confidence: 98%

“…While Tau proteins are known as one of the neuronal MAPs, they are observed to be expressed in several other cells, including both cancerous and normal ovarian and breast cells [13] , [14] , [15] . Since a possible link has been reported between the overexpression of Tau protein and drug resistance, the study of the Tau on cancer microtubules will contribute to new knowledge that may be significant in overcoming drug resistance, leading to impactful therapeutic strategies.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The regulatory effect of Tau protein on polymerization of MCF7 microtubules in vitro

Feizabadi

Hernandez

Breslin

et al. 2019

Biochemistry and Biophysics Reports

View full text Add to dashboard Cite

Growing evidence continues to point toward the critical role of beta tubulin isotypes in regulating some intracellular functions. Changes that were observed in the microtubules’ intrinsic dynamics, the way they interact with some chemotherapeutic agents, or differences on translocation specifications of some molecular motors along microtubules, were associated to their structural uniqueness in terms of beta tubulin isotype distributions. These findings suggest that the effects of microtubule associated proteins (MAPs) may also vary on structurally different microtubules. Among different microtubule associated proteins, Tau proteins, which are known as neuronal MAPs, bind to beta tubulin, stabilize microtubules, and consequently promote their polymerizations.In this study, in a set of well controlled experiments, the direct effect of Tau proteins on the polymerization of two structurally different microtubules, porcine brain and breast cancer (MCF7), were tested and compared. Remarkably, we found that in contrast with the promoted effect of Tau proteins on brain microtubules’ polymerization, MCF7 expressed a demoted polymerization while interacting with Tau proteins. This finding can potentially be a novel insight into the mechanism of drug resistance in some breast cancer cells.It has been reported that microtubules show destabilizing behavior in some MCF7 cells with overexpression of Tau protein when treated with a microtubules’ stabilizing agent, Taxol. This behavior has been classified by others as drug resistance, but it may instead be potentially caused by a competition between the destabilizing effect of the Tau protein and the stabilizing effect of the drug on MCF7 microtubules. Also, we quantified the polarization coefficient of MCF7 microtubules in the presence and absence of Tau proteins by the electro-orientation method and compared the values. The two significantly different values obtained can possibly be one factor considered to explain the effect of Tau proteins on the polymerization of MCF7 microtubules.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The regulatory effect of Tau protein on polymerization of MCF7 microtubules in vitro

Feizabadi

Hernandez

Breslin

et al. 2019

Biochemistry and Biophysics Reports

View full text Add to dashboard Cite

show abstract

“…For example, Tau overexpression observed in breast and ovarian cancer cells was correlated with a poor outcome [293][294][295], while downregulation of Tau in breast and ovarian cancer cell lines increased sensitivity to paclitaxel [293,295]. Because Tau and taxanes bind to the same tubulin surface, it was proposed that Tau may compete with paclitaxel for binding to β-tubulin, causing taxane ineffectiveness [293,295]. On the other hand, in mice, docetaxel-sensitive pancreatic neoplasms show a higher level of Tau and MAP2 with respect to those that are docetaxel-resistant [296,297].…”

Section: Microtubule-associated Proteins and Cancermentioning

confidence: 99%

Intrinsic and Extrinsic Factors Affecting Microtubule Dynamics in Normal and Cancer Cells

et al. 2020

View full text Add to dashboard Cite

Microtubules (MTs), highly dynamic structures composed of α- and β-tubulin heterodimers, are involved in cell movement and intracellular traffic and are essential for cell division. Within the cell, MTs are not uniform as they can be composed of different tubulin isotypes that are post-translationally modified and interact with different microtubule-associated proteins (MAPs). These diverse intrinsic factors influence the dynamics of MTs. Extrinsic factors such as microtubule-targeting agents (MTAs) can also affect MT dynamics. MTAs can be divided into two main categories: microtubule-stabilizing agents (MSAs) and microtubule-destabilizing agents (MDAs). Thus, the MT skeleton is an important target for anticancer therapy. This review discusses factors that determine the microtubule dynamics in normal and cancer cells and describes microtubule–MTA interactions, highlighting the importance of tubulin isoform diversity and post-translational modifications in MTA responses and the consequences of such a phenomenon, including drug resistance development.

show abstract

“…This stabilizes microtubules and influences transportation of cellular secretory proteins. Moreover, MAPT has been reported to accelerate cancer cell growth [ 119 ], while its inactivation through gene knockdown suppressed cell proliferation [ 120 ]. Therefore, it is speculated that the diverse affinity of a natural drug to different functional protein targets may be one of the key factors for different selectivity profiles on VSMCs or VECs.…”

Section: Selective Inhibition Of Vsmcs Versus Vecs Shows Significamentioning

confidence: 99%

Plant‐Derived Products for Treatment of Vascular Intima Hyperplasia Selectively Inhibit Vascular Smooth Muscle Cell Functions

Al-ani

Pan

et al. 2018

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Natural products are used widely for preventing intimal hyperplasia (IH), a common cardiovascular disease. Four different cells initiate and progress IH, namely, vascular smooth muscle, adventitial and endothelial cells, and circulation or bone marrow-derived cells. Vascular smooth muscle cells (VSMCs) play a critical role in initiation and development of intimal thickening and formation of neointimal hyperplasia. In this review, we describe the different originating cells involved in vascular IH and emphasize the effect of different natural products on inhibiting abnormal cellular functions, such as VSMC proliferation and migration. We further present a classification for the different natural products like phenols, flavonoids, terpenes, and alkaloids that suppress VSMC growth. Abnormal VSMC physiology involves disturbance in MAPKs, PI3K/AKT, JAK-STAT, FAK, and NF-κB signal pathways. Most of the natural isolate studies have revealed G1/S phase of cell cycle arrest, decreased ROS production, induced cell apoptosis, restrained migration, and downregulated collagen deposition. It is necessary to screen optimal drugs from natural sources that preferentially inhibit VSMC rather than vascular endothelial cell growth to prevent early IH, restenosis following graft implantation, and atherosclerotic diseases.

show abstract

Functional role of the Tau protein in epithelial ovarian cancer cells

Cited by 15 publications

References 23 publications

The regulatory effect of Tau protein on polymerization of MCF7 microtubules in vitro

The regulatory effect of Tau protein on polymerization of MCF7 microtubules in vitro

Intrinsic and Extrinsic Factors Affecting Microtubule Dynamics in Normal and Cancer Cells

Plant‐Derived Products for Treatment of Vascular Intima Hyperplasia Selectively Inhibit Vascular Smooth Muscle Cell Functions

Contact Info

Product

Resources

About