Functional Signatures of the Epiphytic Prokaryotic Microbiome of Agaves and Cacti

Flores‐Núñez, Víctor M.; Fonseca-García, Citlali; Desgarennes, Damaris; Eloe‐Fadrosh, Emiley A.; Woyke, Tanja; Partida‐Martínez, Laila P.

doi:10.3389/fmicb.2019.03044

Cited by 45 publications

(34 citation statements)

References 57 publications

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“…Furthermore, QS helps in selecting and shaping the bacterial partners with specific PGPR and biocontrol functions thereby benefiting plants to achieve optimum fitness (Hartmann et al, 2014;Altaf et al, 2017;Zaytseva et al, 2019). Such PGPR can be found enriched in the rhizospheres of desert plants such as Agave and cacti (Flores-Núñez et al, 2020).…”

Section: Application Of Desert Pgpr For Sustainable Agriculturementioning

confidence: 99%

Desert Microbes for Boosting Sustainable Agriculture in Extreme Environments

2020

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A large portion of the earth's surface consists of arid, semi-arid and hyper-arid lands. Life in these regions is profoundly challenged by harsh environmental conditions of water limitation, high levels of solar radiation and temperature fluctuations, along with soil salinity and nutrient deficiency, which have serious consequences on plant growth and survival. In recent years, plants that grow in such extreme environments and their naturally associated beneficial microbes have attracted increased interest. The rhizosphere, rhizosheath, endosphere, and phyllosphere of desert plants display a perfect niche for isolating novel microbes. They are well adapted to extreme environments and offer an unexploited reservoir for bio-fertilizers and bio-control agents against a wide range of abiotic and biotic stresses that endanger diverse agricultural ecosystems. Their properties can be used to improve soil fertility, increase plant tolerance to various environmental stresses and crop productivity as well as benefit human health and provide enough food for a growing human population in an environment-friendly manner. Several initiatives were launched to discover the possibility of using beneficial microbes. In this review, we will be describing the efforts to explore the bacterial diversity associated with desert plants in the arid, semi-arid, and hyper-arid regions, highlighting the latest discoveries and applications of plant growth promoting bacteria from the most studied deserts around the world.

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Section: Application Of Desert Pgpr For Sustainable Agriculturementioning

confidence: 99%

Desert Microbes for Boosting Sustainable Agriculture in Extreme Environments

2020

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“…The differences in microbial community structure between wild and cultivated plants have been reported for different species of Agave ( Coleman-Derr et al, 2016 ; Fonseca-García et al, 2018 ) suggesting that cultivation of A. tequilana negatively influences its microbial diversity. A recent report ( Flores-Nuñez et al, 2020 ) approached by shotgun metagenome analysis, pointed again in the same direction; the rhizospheric bacterial community of A. tequilana was less diverse than that of wild A. salmiana , and other wild cacti. Although their cultivated and native non-rhizospheric soil samples showed similar diversities.…”

Section: Discussionmentioning

confidence: 71%

Composition, Structure, and PGPR Traits of the Rhizospheric Bacterial Communities Associated With Wild and Cultivated Echinocactus platyacanthus and Neobuxbaumia polylopha

et al. 2020

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The Queretaro semi-desert in central Mexico is the most southern extension of the Chihuahua desert. This semi-arid zone shelters a vast cactus diversity with many endemic species. Currently, two cacti species from this semi-desert namely, Echinocactus platyacanthus and Neobuxbaumia polylopha are under a threat to their survival. So far, there are no reports on the bacterial communities associated with these plants. In this study, we assessed the structure and diversity of the rhizospheric bacterial communities associated with Echinocactus platyacanthus and Neobuxbaumia polylopha growing in wild and cultivated conditions. Samples of E. platyacanthus were also approached with culture-based methods in search of isolates with plant growth promoting abilities. Metagenomic DNA was extracted from rhizospheric samples and used for Illumina sequencing of the 16S rRNA gene. α-diversity and amplicon sequence variant (ASV) richness were higher in both groups of E. platyacanthus samples. All samples accounted for 14 phyla, and the major 6 were common to all treatments. The dominant phyla in all four sample groups were Actinobacteria and Proteobacteria. Analysis at family and genus levels showed association patterns with the cultivated samples from both species grouping together, while the wild samples of each cactus species were grouping apart. High abundance values of Rubrobacteraceae (15.9-18.4%) were a characteristic feature of wild E. platyacanthus samples. In total, 2,227 ASVs were scored in all 12 rhizospheric samples where E. platyacanthus samples showed higher richness with 1,536 ASVs. Regarding the growing conditions, both groups of cultivated samples were also richer accounting for 743 and 615 ASVs for E. platyacanthus and N. polylopha, respectively. The isolates from E. platyacanthus rhizosphere were mainly assigned to Bacilli and Gammaproteobacteria. In total 35

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“…Specifically, the relative abundance of Pseudoalteromonas in groups A and D decreased more than five-fold after QSI-treatment, compared with that of the control group (group C). Numerous reports have shown that biofilm formation is closely associated with Pseudoalteromonas (a typical bacterium that relies heavily on QS), which could regulate microbial population and help other bacteria colonize [30][31][32]. Like Pseudoalteromonas, Halomonas is also a QS producer that is sensitive to QSI.…”

Section: Discussionmentioning

confidence: 99%

The Rhodamine Isothiocyanate Analogue as a Quorum Sensing Inhibitor Has the Potential to Control Microbially-Induced Biofouling

et al. 2020

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Quorum sensing inhibitors (QSIs) have been proven to be an innovative approach to interfering with biofilm formation, since this process is regulated by QS signals. However, most studies have focused on single-species biofilm formation, whereas studies of the effects of signal interference on the development of multispecies biofilm, especially in the natural environment, are still lacking. Here we develop and evaluate the anti-biofilm capability of a new QSI (rhodamine isothiocyanate analogue, RIA) in natural seawater. During the experiment, biofilm characteristics, microbial communities/functions and network interactions were monitored at 36, 80, and 180 h, respectively. The results showed that the biomass and 3D structure of the biofilm were significantly different in the presence of the QSI. The expression of genes involved in extracellular polysaccharide synthesis was also downregulated in the QSI-treated group. Dramatic differences in microbial composition, β-diversity and functions between the RIA-treated group and the control group were also observed, especially in the early stage of biofilm development. Furthermore, co-occurrence model analysis showed that RIA reduced the complexity of the microbial network. This study demonstrates that rhodamine isothiocyanate analogue is an efficient QS inhibitor and has potential applications in controlling biofouling caused by multispecies biofilm, especially in the early stage of biofouling formation.

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Functional Signatures of the Epiphytic Prokaryotic Microbiome of Agaves and Cacti

Cited by 45 publications

References 57 publications

Desert Microbes for Boosting Sustainable Agriculture in Extreme Environments

Desert Microbes for Boosting Sustainable Agriculture in Extreme Environments

Composition, Structure, and PGPR Traits of the Rhizospheric Bacterial Communities Associated With Wild and Cultivated Echinocactus platyacanthus and Neobuxbaumia polylopha

The Rhodamine Isothiocyanate Analogue as a Quorum Sensing Inhibitor Has the Potential to Control Microbially-Induced Biofouling

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