Functional stability of the nitrate-reducing community in grassland soils towards high nitrate supply

Deiglmayr, Kathrin; Philippot, Laurent; Kandeler, Ellen

doi:10.1016/j.soilbio.2006.04.034

Cited by 17 publications

(15 citation statements)

References 13 publications

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“…It could be argued that the type of fertilizer added (mineral) may have played a role in this experiment as in other studies (Enwall et al ., 2005; Wallenstein et al ., 2006a; Chu et al ., 2007a) and that organic forms may have had a different effect. However, such resilience to a sustained response to mineral fertilization was also described for other soils (Deiglmayr & Kandeler, 2006; Wallenstein et al ., 2006b; Chu et al ., 2007b). It is doubtful whether longer treatment by any type of N‐fertilizer would have drastically changed the C/N ratio in the Dünnwald soil, because the site is consistently exposed to large N inputs from the air.…”

Section: Discussionsupporting

confidence: 53%

Diversity of total, nitrogen‐fixing and denitrifying bacteria in an acid forest soil

Rösch¹,

Bothe²

2009

European J Soil Science

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To assess the diversity of total, denitrifying and N 2 -fixing bacteria in a nitrogen (N)-limited, acid forest soil, isolated DNA was analysed for the genes 16S rRNA, nosZ and nifH. Sequence information for these genes was obtained from clone libraries and from our TReFID computer program, which employs terminal restriction patterns for bacteria using multiple restriction enzymes. Both approaches indicated that Proteobacteria (α-and γ -groups) and Acidobacteria dominated. A comprehensive list of bacteria retrieved from this soil is provided and compared with literature data on the bacterial community compositions from other sites. The study indicated that the current PCR conditions with the primers employed allowed retrieval of only a portion of the bacteria occurring in soils. Massive treatment of a soil plot with NH 4 NO 3 caused an increase in the N content, which was rapidly followed by an enhancement of carbon (C) content. Thus the C/N ratio stayed below 16.0 and the soil remained N-limited. This may explain why the bacterial diversity did not undergo drastic shifts as was tentatively inferred from the available data sets.

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Section: Discussionsupporting

confidence: 53%

Diversity of total, nitrogen‐fixing and denitrifying bacteria in an acid forest soil

Rösch¹,

Bothe²

2009

European J Soil Science

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“…Assessment protocols linked to ecological drivers of wetland microbial communities and their processes are needed to ensure successful delivery of ecosystem services by mitigation banks. Along with hydrology, our results indicate that fertility (e.g., nitrate and carbon availability) is an important indicator of the capacity of wetland soils to support resident microbial communities and denitrifying activity (20,22,40). Microorganisms are responsible for driving nutrient cycling, and understanding their dynamics in response to wetland mitigation activities can offer insight into appropriate management and monitoring of restored areas (33,79).…”

Section: Comparison Of Biotic Communities Among Wetland Typesmentioning

confidence: 84%

Microbial Community Structure and Denitrification in a Wetland Mitigation Bank

Peralta

Matthews

Kent

2010

Appl Environ Microbiol

113

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Wetland mitigation is implemented to replace ecosystem functions provided by wetlands; however, restoration efforts frequently fail to establish equivalent levels of ecosystem services. Delivery of microbially mediated ecosystem functions, such as denitrification, is influenced by both the structure and activity of the microbial community. The objective of this study was to compare the relationship between soil and vegetation factors and microbial community structure and function in restored and reference wetlands within a mitigation bank. Microbial community composition was assessed using terminal restriction fragment length polymorphism targeting the 16S rRNA gene (total bacteria) and the nosZ gene (denitrifiers). Comparisons of microbial function were based on potential denitrification rates. Bacterial community structures differed significantly between restored and reference wetlands; denitrifier community assemblages were similar among reference sites but highly variable among restored sites throughout the mitigation bank. Potential denitrification was highest in the reference wetland sites. These data demonstrate that wetland restoration efforts in this mitigation bank have not successfully restored denitrification and that differences in potential denitrification rates may be due to distinct microbial assemblages observed in restored and reference (natural) wetlands. Further, we have identified gradients in soil moisture and soil fertility that were associated with differences in microbial community structure. Microbial function was influenced by bacterial community composition and soil fertility. Identifying soil factors that are primary ecological drivers of soil bacterial communities, especially denitrifying populations, can potentially aid the development of predictive models for restoration of biogeochemical transformations and enhance the success of wetland restoration efforts.Wetlands provide more ecosystem services (e.g., flood control, water purification, nutrient cycling, and habitat for wildlife) per hectare than any other ecosystem (16). Riparian wetlands, in particular, are sites of intense biogeochemical activity and play an important role in improving water quality, recycling nutrients, and detoxifying chemicals (41). Changing patterns of land use over the last century have resulted in the loss of over half of the wetlands in the contiguous United States (17) and about 60% of wetlands in the Midwestern United States (82). The loss of ecosystem services through conversion of wetlands to alternative (primarily agricultural) land uses exacerbates nutrient pollution and eutrophication of downstream ecosystems (57). Declines in wetland acreage have continued despite a federal policy goal of no-net-loss of wetland acreage and function adopted in 1990 (7, 55). Wetland mitigation projects provide compensation for impacted wetlands and aim to replace the critical functions provided by wetlands. Despite decades of wetland mitigation, however, restoration efforts frequently fail to reestablish desired...

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“…However, in the studies by Deiglmayr et al . () and Enwall et al . () described above, the narG gene (codes for

{NO}_{3}^{-}

reductase) was targeted, suggesting that the lack of response to

{NO}_{3}^{-}

might be more biological than methodological; however, additional studies are necessary to fully understand how

{NO}_{3}^{-}

addition impacts vernal pool denitrifier communities.…”

Section: Discussionmentioning

confidence: 99%

The effects of pH change and NO−3 pulse on microbial community structure and function: a vernal pool microcosm study

Carrino‐Kyker

Smemo

Burke

2012

FEMS Microbiol Ecol

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Forest vernal pools experience strong environmental fluctuations, such as changes in water chemistry, which are often correlated with changes in microbial community structure. However, very little is known about the extent to which these community changes influence ecosystem processes in vernal pools. This study utilized experimental vernal pool microcosms to simulate persistent pH alteration and a pulse input of nitrate (NO3 -), which are common perturbations to temperate vernal pool ecosystems. pH was manipulated at the onset and microbial respiration was monitored throughout the study (122 days). On day 29, NO3 - was added and denitrification rate was measured and bacterial, fungal, and denitrifier communities were profiled on day 30 and day 31. Microbial respiration and both bacterial and fungal community structure were altered by the pH treatment, demonstrating both structural and functional microbial responses. The NO3 - pulse increased denitrification rate without associated changes in community structure, suggesting that microbial communities responded functionally without structural shifts. The functioning of natural vernal pools, which experience both persistent and short-term environmental change, may thus depend on the type and duration of the change or disturbance.

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Functional stability of the nitrate-reducing community in grassland soils towards high nitrate supply

Cited by 17 publications

References 13 publications

Diversity of total, nitrogen‐fixing and denitrifying bacteria in an acid forest soil

Diversity of total, nitrogen‐fixing and denitrifying bacteria in an acid forest soil

Microbial Community Structure and Denitrification in a Wetland Mitigation Bank

The effects of pH change and NO−3 pulse on microbial community structure and function: a vernal pool microcosm study

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