Functional tethered lipid bilayers

Coarse-grained modeling of interactions of lipid bilayers with supports

“…7,8 Even interactions with whole cells have been studied. 9,10 Characterization of supported lipid bilayers has been accomplished with numerous methods including atomic force microscopy, 11 x-ray and neutron scattering and reflectometry, [12][13][14][15] nuclear magnetic resonance, 16 quartz crystal microbalance, 17 surface plasmon resonance, [18][19][20][21] ellipsometry, 6 electrical impedance spectroscopy, 15 and many versions of fluorescence microscopy such as fluorescence resonance energy transfer, 22 fluorescence recovery after photobleaching, 23 fluorescence interference contrast, 24,25 fluorescence correlation spectroscopy, 26 and total internal reflection fluorescence. 27 Phospholipid bilayers adsorb to hydrophilic surfaces to create a supported bilayer leaving a layer of water up to 5 Å thick-in addition to the hydration shell of the head groups-between the substrate and the head groups of the lipids in the proximal ͑closest to the solid͒ leaflet.…”

“…To address this problem several methods have been developed to increase the spacing between the bilayer and substrate. 15,18,[28][29][30] Nonetheless, measurements of pure and mixed lipid membranes performed directly on supports remain a valuable source of information on the nature of lipid-lipid and lipid-solvent interactions.…”

Nano for bio: Nanopore arrays for stable and functional lipid bilayer membranes (Mini Review)

“…12,13 Since BLMs are notoriously fragile, 14 a race began in the late 1980s to make them more stable. Several groups started to immobilize lipid bilayers on solid surfaces, [15][16][17] aimed at achieving systems suitable for analytical applications. 18 In developing stable and functional supported bilayers, one main topic of the TETH-MEM conference meetings, researchers encountered the difficulties of squaring the circle.…”

Attempts to prepare tethered bilayer lipid membranes using synthetic thioglycolipid anchors: synthesis of 6″-thiotrisaccharide glycolipid analogues and applications

“…Anchoring systems include thiols, sulfides or disulfides on silver or gold and trichloro-or trimethoxysilanes on oxide surfaces such as SiO 2 or TiO 2 . 5 Lipoglycopolymeric tethers have been designed 6 but the concept of a purely carbohydrate based tether system is novel. Such a tethering system would mimic the properties of the glycocalyx by providing stabilization through hydrogen bonding networks and maintaining osmotic pressure within the compartment.…”