Functional units in the labellar chemosensory hairs of the mosquito <i>Culiseta inornata</i> (Williston)

Owen, William B.; Larsen, Joseph R.; Pappas, Larry G.

doi:10.1002/jez.1401880211

Cited by 15 publications

(15 citation statements)

References 26 publications

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“…Whereas 1mmoll -1 KCl is used as the reference to characterize the water cell in labellar and tarsal taste sensilla of D. melanogaster (Hiroi et al, 2004;Meunier et al, 2009), the labellar neurone of Anopheles appears to be more sensitive to KCl as this concentration reduced the number of spikes generated by the water cell of the T1 sensillar receptor cells by 40%. Water, salt and sugar neurones have been identified in labellar trichoid T1 of C. inornata (Owen et al, 1974;Pappas and Larsen, 1976). The functionality of the lateral labellar T1 sensillum neurones in A. gambiae on which this study focused seems to be similar as separate sugar and water neurones have been identified in the present study.…”

Section: Sensing and Feeding On Sucrose By A Gambiaesupporting

confidence: 78%

The sugar meal of the African malaria mosquito Anopheles gambiae (Giles) and how deterrent compounds interfere with it: a behavioural and neurophysiological study

Kessler

Vlimant

Guérin

2012

Journal of Experimental Biology

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, but not by the same concentrations of caffeine. In conclusion, sucrose stimulates feeding and activates the labellar sucrose neurone, whereas feeding deterrents inhibit both the sucrose and water neurones. This study provides an initial understanding of the physiological mechanisms involved in sugar feeding in A. gambiae and shows how some bitter products interfere with it.Supplementary material available online at

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Section: Sensing and Feeding On Sucrose By A Gambiaesupporting

confidence: 78%

The sugar meal of the African malaria mosquito Anopheles gambiae (Giles) and how deterrent compounds interfere with it: a behavioural and neurophysiological study

Kessler

Vlimant

Guérin

2012

Journal of Experimental Biology

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“…3A and Figs. S5A, S8A, and S9A), are identical to the long labellar sensilla described in A. aegypti (28,29) and are similar to the long labellar sensilla (trichoid) of Anopheles gambiae (30) and Culiseta inornata (31,32). Accessory cells surrounding sensory neurons immunostained for the Aedae-KR could be the trichogen or tormogen (30,33), because these cells are believed to secrete the dendrite bathing fluid (34,35).…”

Section: Discussionsupporting

confidence: 55%

Leucokinin mimetic elicits aversive behavior in mosquito Aedes aegypti (L.) and inhibits the sugar taste neuron

Kwon

Agha

Smith

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Insect kinins (leucokinins) are multifunctional peptides acting as neurohormones and neurotransmitters. In females of the mosquito vector Aedes aegypti (L.), aedeskinins are known to stimulate fluid secretion from the renal organs (Malpighian tubules) and hindgut contractions by activating a G protein-coupled kinin receptor designated "Aedae-KR." We used protease-resistant kinin analogs 1728, 1729, and 1460 to evaluate their effects on sucrose perception and feeding behavior. In no-choice feeding bioassays (capillary feeder and plate assays), the analog 1728, which contains α-amino isobutyric acid, inhibited females from feeding on sucrose. It further induced quick fly-away or walk-away behavior following contact with the tarsi and the mouthparts. Electrophysiological recordings from single long labellar sensilla of the proboscis demonstrated that mixing the analog 1728 at 1 mM with sucrose almost completely inhibited the detection of sucrose. Aedae-KR was immunolocalized in contact chemosensory neurons in prothoracic tarsi and in sensory neurons and accessory cells of long labellar sensilla in the distal labellum. Silencing Aedae-KR by RNAi significantly reduced gene expression and eliminated the feeding-aversion behavior resulting from contact with the analog 1728, thus directly implicating the Aedae-KR in the aversion response. To our knowledge, this is the first report that kinin analogs modulate sucrose perception in any insect. The aversion to feeding elicited by analog 1728 suggests that synthetic molecules targeting the mosquito Aedae-KR in the labellum and tarsi should be investigated for the potential to discover novel feeding deterrents of mosquito vectors.neuropeptide GPCR | sucrose taste | sensory neuron | chemical target validation | feeding deterrent

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“…But many workers [1,3,4,11,13,15] have found only double-chambered hairs, and some of these workers have concluded that all aboral hairs beyond a certain length (e.g. 32 m in C. inornata as reported by Owen [3]) are chemosensory.…”

Section: Long Labellar Hairsmentioning

confidence: 99%

“…In probing to find a suitable spot for feeding, the mosquito uses the two labellar lobes located at the tip of the long, gutter-like labium [7][8][9]. The structure of the labellar sense organs in mosquitoes has been studied using light microscopy (LM) [1-4, 10, 11], scanning electron microscopy (SEM) [12], and transmission electron microscopy (TEM) [5,[13][14][15]. The limitation of these studies was that only some sense organs were studied, i.e.…”

Section: Introductionmentioning

confidence: 99%

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Fine Structure of the Sense Organs on the Labella and Labium of the Mosquito Aedes aegypti (L.)

Lee¹,

Craig²

2009

TOENTOJ

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Fine structure of the sense organs on the labella and labium of male and female mosquito Aedes aegypti is described. Labellar hair on the outside of the two labellar lobes are consisted of long mechanoreceptive hairs, medium-sized chemoreceptive hairs containing 3-5 dendrites, and short papillae which are probably olfactory receptors. Two apical hairs each containing five dendrites not reported before are found deeply embedded inside each labellum. They emerged between folds at the tip of the labellum. These and other sensory hairs on the outside of the labella are probably involved in finding a suitable place for feeding after a mosquito has landed on a host. Six anteriorly directed papillae each containing 3-5 dendrites are found on the oral surface of each labellar lobe with no evidence of mechanoreceptors associated with these papillae. These papilla are probably chemosensory and are involved in detecting the food entering the food canal when a mosquito feed on water and other liquid diet such as nectar. A chordotonal organ with two sensory cells is found inside each labellum, and this organ has not been described on mosquito mouthparts before. These chordotonal organs probably function to monitor the spreading and closing of the labellar lobes during feeding. Mosquito spread their labellar lobes when feeding on water and sugar but these lobes are firmly pressed against each other when they feed on blood. Ligular hairs are definitely not sensory because of a lack any dendrites inside these hairs. Labial hairs proximal to the labella are probably mechanoreceptors because only one nerve cell is associated with each hair with nerve terminating at the base of the hair. Based on results from behavioral and functional studies, the function of these sensilla during feeding is described.

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Functional units in the labellar chemosensory hairs of the mosquito Culiseta inornata (Williston)

Cited by 15 publications

References 26 publications

The sugar meal of the African malaria mosquito Anopheles gambiae (Giles) and how deterrent compounds interfere with it: a behavioural and neurophysiological study

The sugar meal of the African malaria mosquito Anopheles gambiae (Giles) and how deterrent compounds interfere with it: a behavioural and neurophysiological study

Leucokinin mimetic elicits aversive behavior in mosquito Aedes aegypti (L.) and inhibits the sugar taste neuron

Fine Structure of the Sense Organs on the Labella and Labium of the Mosquito Aedes aegypti (L.)

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