Functional Up-Conversion Nanoparticle-Based Immunochromatography Assay for Simultaneous and Sensitive Detection of Residues of Four Tetracycline Antibiotics in Milk

Abstract

An ultrahigh-sensitivity lateral flow immunochromatography (LFIC) assay based on up-converting nanoparticles (UCNPs) was developed to carry out a multi-residue detection of tetracycline in milk. The sensitivity of the immunoassay was greatly improved by the use of a broad-spectrum monoclonal antibody attached to UCNPs to form a signal probe. Under the optimal conditions, the UCNP-LFIC assay enabled sensitive detection of tetracycline (TC) as well as of oxytetracycline (OTC), chlortetracycline (CTC), and doxycycline (DOX) within 10 min, with IC 50 values of 0.32, 0.32, 0.26, 0.22 ng/mL, respectively. There was no cross-reactivity with ten other antibiotics. Similarly, we evaluated the experimental results for matrix effects. Experiments involving spiking showed the four tetracycline antibiotics displaying mean recoveries ranging from 93.95 to 111.90% with relative standard deviations (RSDs) of < 9.95%. The detection results of actual samples using the developed method showed a good correlation ( R 2 ≥ 0.98) with the results using high-performance liquid chromatography (HPLC). Thus, the assay can achieve an ultrahighly sensitive detection of antibiotics in milk, and can hence promote human health and provides promising applications in the bio-detection field.

Ordered Porous Layer Interferometry for Dynamic Observation of Non-Specific Adsorption Induced by 1-Ethyl-3-(3-(dimethylamino)propyl) Carbodiimide

“…38 With a conjugated system and abundant amino groups, CTC can be a candidate to attach to BSA by EDC chemistry and can be characterized by a UV−vis spectrophotometer. 39 Furthermore, the reports from Taheran et al 40 and Parolo et al 41 suggested CTC possessed net negative charges around pH 7, enhancing electrostatic repulsion and restraining the adsorption between CTC and a negatively charged material. According to their work, the interaction between negatively charged CTC and negatively charged BSA-blocked silica colloidal crystal (SCC) film was mainly forced by electrostatic repulsion at pH 7.2, the condition used in our experiments.…”

“…CTC is widely used in livestock, poultry, and aquatic organisms with the advantage of economic savings . With a conjugated system and abundant amino groups, CTC can be a candidate to attach to BSA by EDC chemistry and can be characterized by a UV–vis spectrophotometer . Furthermore, the reports from Taheran et al and Parolo et al suggested CTC possessed net negative charges around pH 7, enhancing electrostatic repulsion and restraining the adsorption between CTC and a negatively charged material.…”

Fluorescent immunochromatography of vancomycin in plasma: A rapid and sensitive quantitative analysis

“…A novel method of membrane detection, FICA, combines chromatographic chromatography with immunological methods (Pyo & Yoo, 2012). The advantages of low cost, ease of use, quick reaction, and high sensitivity have allowed this technique to be widely used in food and drug analysis (Wang et al, 2016; Xu et al, 2020; Zhang et al, 2020). FICA determination offers a practical and reliable method for clinical diagnosis.…”

Latex Microsphere-Based Bicolor Immunochromatography for Qualitative Detection of Neutralizing Antibody against SARS-CoV-2

“…A lateral flow immunoassay (LFIA), which combines chromatography technology with conventional immunoassay and nanomaterials, is considered the most attractive point-of-care testing device, because it exhibits several advantages, including simple technical requirements, rapid detection capability, portability, affordability, high detection accuracy, and high efficiency [ 25 ]. LFIAs have been widely applied in detecting pesticide [ 26 , 27 ] and veterinary drugs residues [ 28 , 29 ], toxicants [ 30 , 31 , 32 ], pathogens [ 32 , 33 ], various diseases biomarkers, as well as SARS-CoV-2 antigen/antibody [ 34 , 35 ]. Usually, the results of a LFIA are presented with the test and control lines showing the same color, resulting in it not being easy for users to discern the locations of test and control zones.…”