Functionalisation of calcium phosphate nanoparticles by oligonucleotides and their application for gene silencing

Sokolova, Viktoriya; Kovtun, Anna; Prymak, Oleg; Meyer‐Zaika, Wolfgang; Кубарева, Е. А.; Романова, Е. А.; Oretskaya, Tatiana S.; Heumann, Rolf; Epple, Matthias

doi:10.1039/b612699e

Cited by 105 publications

(97 citation statements)

References 36 publications

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“…The polymers have pK a values of 4.0 (carboxylic acid group) and 8.6 to 9.7 (amino group). or drug or gene delivery [5,[36][37][38][39]. As a result, although the amounts of polymeric additive needed for an individual application are not very large, the overall need for a special polymer may still be rather large, considering that toothpastes and mouthwash products for dental protection are an ever growing market [40,41].…”

Section: Resultsmentioning

confidence: 99%

“…However, besides a few water-soluble cellulose derivatives that are available on a commercial scale [26], the number of specific and tailor-made additives for calcium phosphate precipitation is still rather limited. This is interesting because calcium phosphate mineralization control or delivery of (polymer stabilized) calcium phosphate nanoparticles are interesting for e.g., dental repair (remineralization), toothpastes, or drug or gene delivery [5,[36][37][38][39]. As a result, although the amounts of polymeric additive needed for an individual application are not very large, the overall need for a special polymer may still be rather large, considering that toothpastes and mouthwash products for dental protection are an ever growing market [40,41].…”

Section: Introductionmentioning

confidence: 99%

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Water-Soluble Cellulose Derivatives Are Sustainable Additives for Biomimetic Calcium Phosphate Mineralization

et al. 2016

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Abstract:The effect of cellulose-based polyelectrolytes on biomimetic calcium phosphate mineralization is described. Three cellulose derivatives, a polyanion, a polycation, and a polyzwitterion were used as additives. Scanning electron microscopy, X-ray diffraction, IR and Raman spectroscopy show that, depending on the composition of the starting solution, hydroxyapatite or brushite precipitates form. Infrared and Raman spectroscopy also show that significant amounts of nitrate ions are incorporated in the precipitates. Energy dispersive X-ray spectroscopy shows that the Ca/P ratio varies throughout the samples and resembles that of other bioinspired calcium phosphate hybrid materials. Elemental analysis shows that the carbon (i.e., polymer) contents reach 10% in some samples, clearly illustrating the formation of a true hybrid material. Overall, the data indicate that a higher polymer concentration in the reaction mixture favors the formation of polymer-enriched materials, while lower polymer concentrations or high precursor concentrations favor the formation of products that are closely related to the control samples precipitated in the absence of polymer. The results thus highlight the potential of (water-soluble) cellulose derivatives for the synthesis and design of bioinspired and bio-based hybrid materials.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Water-Soluble Cellulose Derivatives Are Sustainable Additives for Biomimetic Calcium Phosphate Mineralization

et al. 2016

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“…Bhakta et al used magnesium phosphate nanoparticles to transfect HeLa cells and achieved a transfection efficiency comparable to that of Polyfect ® [22]. The nanoparticles can also be functionalized with siRNA or oligonucleotides as well as DNA [23]. In this case the successful transfection will lead to the inhibition of the production of the desired protein due to its mRNA cleavage.…”

Section: Advanced Bioceramics In Nanomedicine and Tissue Engineeringmentioning

confidence: 99%

Functionalized Calcium Phosphate Nanoparticles for Biomedical Application

Epple

Kovtun

2010

KEM

Self Cite

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Calcium phosphate is a natural biomineral and therefore possesses an excellent biocompatibility due to its chemical similarity to human hard tissue (bone and teeth). Calcium phosphate nanoparticles can be precipitated under controlled conditions and used as carrier in biological systems, e.g. to transfer nucleic acids or drugs. Such nanoparticles can also be suitably functionalized with fluorescing dyes, polymeric agents, pro-drugs or activators. The small monodisperse nanoparticles only mildly influence the intracellular calcium level and therefore are not toxic for cells.

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“…As nucleic acids alone are unable to penetrate the cell wall, efficient carriers are required [728,729]. Nanodimensional calcium orthophosphates can be represented as a unique class of the non-viral vectors, which can serve as efficient and alternative DNA carriers for targeted delivery of genes [289,696,697,708,714,[730][731][732][733][734][735][736][737][738][739][740][741][742] and cells [567,[743][744][745][746][747][748][749]. The standard transfection method using calcium orthophosphates, first introduced by Graham and van der Eb in 1973 [748], is still used in biochemistry.…”

Section: Other Applicationsmentioning

confidence: 99%

Nanodimensional and Nanocrystalline Calcium Orthophosphates

Dorozhkin¹

2012

AJBE

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Nano-sized particles and crystals play an important role in the formation of calcified tissues of various animals. For example, nano-sized and nanocrystalline calcium orthophosphates in the form of apatites of biological origin represent the basic inorganic building blocks of bones and teeth of mammals. Namely, according the recent developments in biomineralization, tens to hundreds nanodimensional crystals of a biological apatite are self-assembled into these complex structures. This process occurs under a strict control by bioorganic matrixes. Furthermore, both a greater viability and a better proliferation of various types of cells have been detected on smaller crystals of calcium orthophosphates. Thus, the nano-sized and nanocrystalline forms of calcium orthophosphates have a great potential to revolutionize the hard tissue-engineering field, starting from bone repair and augmentation to controlled drug delivery systems. This review reports on current state of the art and recent developments on the subject, starting from synthesis and characterization to biomedical and clinical applications. Furthermore, the review also discusses possible directions for future research and development.

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Functionalisation of calcium phosphate nanoparticles by oligonucleotides and their application for gene silencing

Cited by 105 publications

References 36 publications

Water-Soluble Cellulose Derivatives Are Sustainable Additives for Biomimetic Calcium Phosphate Mineralization

Water-Soluble Cellulose Derivatives Are Sustainable Additives for Biomimetic Calcium Phosphate Mineralization

Functionalized Calcium Phosphate Nanoparticles for Biomedical Application

Nanodimensional and Nanocrystalline Calcium Orthophosphates

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