Functionalization of Ag Nanoparticles by Glutamic Acid and Conjugation of Ag@Glu by Thiosemicarbazide Enhances the Apoptosis of Human Breast Cancer MCF-7 Cells

Shandiz, Seyed Ataollah Sadat; Montazeri, Ahmad; Abdolhosseini, Mansoreh; Shahrestani, Somayeh Hadad; Hedayati, Mohammad; Moradi‐Shoeili, Zeinab; Salehzadeh, Ali

doi:10.1007/s10876-018-1424-0

Cited by 27 publications

(11 citation statements)

References 23 publications

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“…The IC50 was significantly low (9.87 μg/ml). The Ag@Glu/TSC induces apoptosis in 69.6% MCF-7 cells while the AgNPs of this study induced apoptosis in 34.4% A549 cells (Shandiz et al 2018 ).…”

Section: Discussionmentioning

confidence: 59%

Anti-cancer & anti-metastasis properties of bioorganic-capped silver nanoparticles fabricated from Juniperus chinensis extract against lung cancer cells

Noorbazargan¹,

amintehrani²,

Dolatabadi³

et al. 2021

AMB Expr

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The current study evaluated the anti-cancer properties of bio-functionalized silver nanoparticles fabricated by Juniperus chinensis leaf extracts. The nanoparticles were characterized by scanning electron microscopy, transmission electron microscopy, UV–visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, dynamic light scattering, Zeta potential and X-ray spectroscopy. Further, this study elucidated the cellular and molecular mechanisms of nanoparticles for anti-proliferative and apoptotic effects on human lung cancer cells (A549) and compared them with commercial drug cisplatin. The size of the spherical nanoparticle was 12.96 nm with negative zeta potential. Up-regulation of caspase 3,9 and p53, Annexin V-FITC/PI, DAPI staining, and ROS production indicated the remarkable apoptotic effect of AgNPs compared to cisplatin. Moreover, down-regulation of MMP2/MMP9 scratch and matrigel assays revealed anti-metastatic properties of AgNPs. Cell cycle analysis and downregulation of cyclin D1 indicated cancer cell cessation in the G0/G1 phase. Overall, the results revealed that the green-synthetized AgNPs had anti-metastasis and anti-proliferation effects on lung cancer cells in comparison to cisplatin with lower side effects on the normal cell line.

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Section: Discussionmentioning

confidence: 59%

Anti-cancer & anti-metastasis properties of bioorganic-capped silver nanoparticles fabricated from Juniperus chinensis extract against lung cancer cells

Noorbazargan¹,

amintehrani²,

Dolatabadi³

et al. 2021

AMB Expr

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“…Previous studies showed that conjugation of TSC to ZnO nanoparticles (NPs) could enhance antibacterial potential of the NPs and showed synergism with antibiotics (Nejabatdoust et al 2019a, b). In addition, conjugation of TSC with Ag NPs and their effectiveness against cancer cells have been reported previously (Shandiz et al 2018). Thus, in this study, we aimed to investigate the possible synergism of Ag NPs conjugated to TSC (Ag-TSC NPs) with ciprofloxacin and their effects on the expression of mexA and mexB genes against ciprofloxacin resistant P. aeruginosa.…”

Section: Introductionmentioning

confidence: 95%

Synergistic antimicrobial potential of ciprofloxacin with silver nanoparticles conjugated to thiosemicarbazide against ciprofloxacin resistant Pseudomonas aeruginosa by attenuation of MexA-B efflux pump genes

2019

Self Cite

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Pseudomonas aeruginosa is an important nosocomial pathogen. Metal nanoparticles (NPs) have shown a promising antibacterial activity against multidrug resistant strains. The purpose of this study was to investigate the synergistic antibacterial effect of silver nanoparticles functionalized by Thiosemicarbazid with ciprofloxacin and their effect on the expression of mexA-B efflux pump genes in ciprofloxacin resistant P. aeruginosa. Ciprofloxacin resistant P. aeruginosa strains were isolated from clinical specimens. Silver nanoparticles (Ag NPs) were functionalized using glutamic acid and conjugated with Thiosemicarbazid (TSC). Antibacterial synergism of Ag-TSC NPs with ciprofloxacin against P. aeruginosa and possible effect of the NPs on the expression of MexA and MexB genes were evaluated using quantitative PCR. The Ag-TSC NPs (concentration ≥ 32 μg/mL) displayed synergistic effects with ciprofloxacin (at sub-MIC concentration), which efficiently inhibited bacterial growth. Expression of mexA and mexB genes was reduced among the strains that simultaneously were exposed to the Ag -TSC NPs and ciprofloxacin by 6.0 and 2.75 folds, respectively, compared to the controls. In this study synergism of Ag-TSC NPs with ciprofloxacin against multi-drug P. aeruginosa by attenuation of ciprofloxacin resistance genes was observed, which could be promising to be used in antibacterial approaches. KeywordsEfflux pumps . Pseudomonas aeruginosa . Silver nanoparticles . Thiosemicarbazide Abbreviations TSC thiosemicarbazide NPs nanoparticles MIC minimum inhibitory concentration CIP ciprofloxacin ROS reactive oxygen species qPCR Quantitative polymerase chain polymerization * Hojjatolah Zamani

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“…A high concentration of Ag-containing coating was used, which revealed the dose-dependent manner of inferior biocompatibility. 27,28 The development of green methods for nanomaterial synthesis is another important aspect of research in the field of nanotechnology. The reported biosynthesis of Fe 3 O 4 @Ag nanocomposite by Spirulinaplatensis cyanobacterium impacts the expression of efflux pump genes and also shows effective antibacterial activity against antibiotic-resistant ciprofloxacin-resistant S. aureus (CRSA) in medicine.…”

Section: Discussionmentioning

confidence: 99%

<p>Beneficial Effect of TaON-Ag Nanocomposite Titanium on Antibacterial Capacity in Orthopedic Application</p>

Chang

et al. 2020

IJN

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In this study, a novel oxygenated nanocomposite thin film, TaON-Ag, was investigated in vitro and in vivo to evaluate its biocompatibility and antibacterial ability. Material and Methods: The antibacterial ability of TaON-Ag nanocomposite-coated titanium (Ti) was evaluated using the Kirby-Bauer disk diffusion susceptibility test. The effects of TaON-Ag nanocomposite-coated metal on osteogenesis were further evaluated in an in vitro osteogenic culture model with rat marrow-derived mesenchymal stem cells (rMSCs). Furthermore, titanium rods coated with TaON-Ag were implanted into a rat femur fracture model either with or without osteomyelitis to investigate the effects of TaON-Ag in osteogenesis. Results: The TaON-Ag-coated Ti exhibited an effective antibacterial effect against Staphylococcus aureus, coagulase-negative Staphylococcus, and the Gram-negative strains Escherichia coli and Pseudomonas aeruginosa. Using an osteogenic culture with rMSCs and a rat femoral fracture model, the TaON-Ag-coated Ti did not interfere with the ossification of rMSCs in vitro or during fracture healing in vivo. Field-emission scanning electron microscopy (FE-SEM) revealed that coating with TaON-Ag could inhibit pathogen adhesion and biofilm formation in both Staphylococcus aureus and Escherichia coli. Conclusion: Using the proposed novel oxygenation process, TaON-Ag nanocomposite-coated Ti yielded robust biocompatibility and antibacterial ability against common microorganisms in orthopedic infections, thereby demonstrating potential for use in clinical applications.

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Functionalization of Ag Nanoparticles by Glutamic Acid and Conjugation of Ag@Glu by Thiosemicarbazide Enhances the Apoptosis of Human Breast Cancer MCF-7 Cells

Cited by 27 publications

References 23 publications

Anti-cancer & anti-metastasis properties of bioorganic-capped silver nanoparticles fabricated from Juniperus chinensis extract against lung cancer cells

Anti-cancer & anti-metastasis properties of bioorganic-capped silver nanoparticles fabricated from Juniperus chinensis extract against lung cancer cells

Synergistic antimicrobial potential of ciprofloxacin with silver nanoparticles conjugated to thiosemicarbazide against ciprofloxacin resistant Pseudomonas aeruginosa by attenuation of MexA-B efflux pump genes

<p>Beneficial Effect of TaON-Ag Nanocomposite Titanium on Antibacterial Capacity in Orthopedic Application</p>

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