Functionalization of atomic force microscopy cantilevers and tips by activated vapour silanization

Daza, Rafael; Colchero, Luis; Corregidor, Daniel; Elices, M.; Guinea, Gustavo V.; Rojo, Francisco Javier; Pérez‐Rigueiro, José

doi:10.1016/j.apsusc.2019.04.155

Cited by 6 publications

(4 citation statements)

References 33 publications

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“…The second method, known as tip silanization (usually amination), can be achieved through the use of (3-aminopropyl)triethoxysilane (APTES) or activated vapor silanization (AVS) . After silanization, polyethylene glycol (PEG) with dual functionality can be used to connect the functionalized tip and the target molecule. ,− Alternatively, by silanizing the tip and coupling cyclooctyne, the target molecule can be attached to the AFM tip through alkyne–azide click chemistry …”

Section: Preparation and Modification Of Afm Probe Tipmentioning

confidence: 99%

Applications of AFM in Membrane Characterization and Fouling Analysis

Liu,

Zhu,

Chen

et al. 2024

ACS EST Eng.

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Atomic force microscopy (AFM), as a type of scanning probe microscopy (SPM), possesses formidable capabilities for nanoscale imaging and force spectroscopy. Due to its advantages such as high resolution, nondestructive detection, minimal environmental restrictions, strong versatility, and real-time in situ analysis, AFM has become an indispensable tool in surface science and materials research, finding extensive applications in the study of the membrane separation and fouling processes. The tremendous advantages of AFM in characterization applications stem from its diverse tip functionalization techniques. This review encompasses the preparation of AFM probe tips and the modification techniques of special tips, including carbon nanotube (CNT) probes, metal nanowire probes, colloidal probes, and single-cell/molecule probes. Furthermore, it highlights the applications and advancements of AFM and probe modification techniques in membrane technology research. With the continuous development of tip modification techniques, the analytical capabilities of AFM will be further expanded, promising broader prospects for its application in the study of membrane fouling mechanisms and the development of antifouling membrane materials.

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Section: Preparation and Modification Of Afm Probe Tipmentioning

confidence: 99%

Applications of AFM in Membrane Characterization and Fouling Analysis

Liu,

Zhu,

Chen

et al. 2024

ACS EST Eng.

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“…Various functionalization techniques can be employed by taking into account the specific molecules involved and the experimental requirements [46]. Dazaet al [47] explored the use of the activated vapor silanization (AVS) process to functionalize AFM cantilevers and tips. Thin films with specific desired functionalities were deposited onto silicon nitride chips, and their existence and performance were confirmed by assessing changes in the resonance frequency of the cantilever.…”

Section: Functionalization Of Afm Tipsmentioning

confidence: 99%

Atomic Force Microscopy as a Tool to Study Transport Phenomena in Biological Systems

Kandapal

2023

Processes

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Biological interactions often involve the transport of molecules, ions, or other species across biological membranes or between interacting proteins. The understanding of these transport phenomena is crucial for the development of therapies for various diseases. Atomic force microscopy is a powerful tool that has been increasingly used to study biological systems at the nano scale. The high resolution, quantitative measurements, and the ability to probe biological interactions under near-physiological conditions make AFM an attractive tool for investigating transport phenomena in biological systems. In this article, we focus on the use of AFM in the study of the transport phenomena in biological systems. We discuss the principles of AFM, its instrumentation, and its application in the study of biomolecules and biological systems. We also provide a comprehensive overview of recent articles that have utilized AFM in the study of biomarkers in biological systems.

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“…A number of techniques have been proposed to functionalize the AFM microcantilevers for use as chemical or biological sensors. For example, Daza et al [ 219 ] attempted the functionalization of a reliable and robust AFM microcantilever tips by using the activated vapor silanization (AVS) process. The functionalized tips were able to withstand repetitive interactions with a model graphite substrate under relatively harsh conditions with no damages to the tip.…”

Section: Fabrication Modification and Functionalization Of Afm Mmentioning

confidence: 99%

“…This activation step is supposed to create a high density of hydroxyl groups on the surface to which the APTES molecules may bind covalently. Even though PECVD-functionalization allows deposition of thick layers, the thickness of the functionalized thin film tends to be restricted to a few nanometers (5–10 nm) [ 219 ].…”

Section: Fabrication Modification and Functionalization Of Afm Mmentioning

confidence: 99%

Review: Cantilever-Based Sensors for High Speed Atomic Force Microscopy

Alunda¹,

Lee

2020

Sensors

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This review critically summarizes the recent advances of the microcantilever-based force sensors for atomic force microscope (AFM) applications. They are one the most common mechanical spring–mass systems and are extremely sensitive to changes in the resonant frequency, thus finding numerous applications especially for molecular sensing. Specifically, we comment on the latest progress in research on the deflection detection systems, fabrication, coating and functionalization of the microcantilevers and their application as bio- and chemical sensors. A trend on the recent breakthroughs on the study of biological samples using high-speed atomic force microscope is also reported in this review.

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Functionalization of atomic force microscopy cantilevers and tips by activated vapour silanization

Cited by 6 publications

References 33 publications

Applications of AFM in Membrane Characterization and Fouling Analysis

Applications of AFM in Membrane Characterization and Fouling Analysis

Atomic Force Microscopy as a Tool to Study Transport Phenomena in Biological Systems

Review: Cantilever-Based Sensors for High Speed Atomic Force Microscopy

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