Functionalization of Cellular Membranes with DNA Nanotechnology

Schoenit, Andreas; Cavalcanti‐Adam, Elisabetta Ada; Göpfrich, Kerstin

doi:10.1016/j.tibtech.2021.02.002

Cited by 24 publications

(21 citation statements)

References 86 publications

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“…Andersen et al constructed a 42 × 36 × 36 nm 3 cubic box from six DNA sheets, which were fabricated by folding the same circular single-stranded M13 phage genomic DNA with staple DNA strands, and remarkably, the lib of this box can be opened with external key oligonucleotides (Andersen et al, 2009). Gorssi et al designed a DNA nanovault device, which also uses M13 phage genomic DNA as folding scaffold, and this device can be programmed to control enzyme-substrate interactions by inducible conformational changes (Figure 3D) (Schoenit et al, 2021). Shih group applied stapled long ssDNA derived from M13 phage genome to build a series of honeycomb-pleated 3D nanostructures with precisely controlled dimensions and various complex shapes, including monolith, railed bridge, stacked cross, slotted cross, genie bottle and square nut-like shapes (Figure 3E) (Douglas et al, 2009).…”

Section: Application Of Dna Framework In the Construction Of Nanostructuresmentioning

confidence: 99%

Application of Nucleic Acid Frameworks in the Construction of Nanostructures and Cascade Biocatalysts: Recent Progress and Perspective

Zhu

Song

et al. 2022

Front. Bioeng. Biotechnol.

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Nucleic acids underlie the storage and retrieval of genetic information literally in all living organisms, and also provide us excellent materials for making artificial nanostructures and scaffolds for constructing multi-enzyme systems with outstanding performance in catalyzing various cascade reactions, due to their highly diverse and yet controllable structures, which are well determined by their sequences. The introduction of unnatural moieties into nucleic acids dramatically increased the diversity of sequences, structures, and properties of the nucleic acids, which undoubtedly expanded the toolbox for making nanomaterials and scaffolds of multi-enzyme systems. In this article, we first introduce the molecular structures and properties of nucleic acids and their unnatural derivatives. Then we summarized representative artificial nanomaterials made of nucleic acids, as well as their properties, functions, and application. We next review recent progress on constructing multi-enzyme systems with nucleic acid structures as scaffolds for cascade biocatalyst. Finally, we discuss the future direction of applying nucleic acid frameworks in the construction of nanomaterials and multi-enzyme molecular machines, with the potential contribution that unnatural nucleic acids may make to this field highlighted.

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Section: Application Of Dna Framework In the Construction Of Nanostructuresmentioning

confidence: 99%

Application of Nucleic Acid Frameworks in the Construction of Nanostructures and Cascade Biocatalysts: Recent Progress and Perspective

Zhu

Song

et al. 2022

Front. Bioeng. Biotechnol.

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“…Introduced 15 years ago, DNA origami technology [ 1 , 2 ] has evolved into a popular nanofabrication method that nowadays is almost routinely employed in numerous areas of biomedical research [ 3 , 4 ], single-molecule biochemistry [ 5 , 6 , 7 ] and biophysics [ 8 , 9 ], bioanalytics and biosensing [ 10 , 11 ], and synthetic biology [ 12 , 13 ], among others. It relies on the controlled folding of a long single-stranded DNA scaffold into a user-defined 2D or 3D nanoscale shape upon hybridization with a large set of short oligonucleotides called staple strands.…”

Section: Introductionmentioning

confidence: 99%

Magnesium-Free Immobilization of DNA Origami Nanostructures at Mica Surfaces for Atomic Force Microscopy

et al. 2021

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DNA origami nanostructures (DONs) are promising substrates for the single-molecule investigation of biomolecular reactions and dynamics by in situ atomic force microscopy (AFM). For this, they are typically immobilized on mica substrates by adding millimolar concentrations of Mg2+ ions to the sample solution, which enable the adsorption of the negatively charged DONs at the like-charged mica surface. These non-physiological Mg2+ concentrations, however, present a serious limitation in such experiments as they may interfere with the reactions and processes under investigation. Therefore, we here evaluate three approaches to efficiently immobilize DONs at mica surfaces under essentially Mg2+-free conditions. These approaches rely on the pre-adsorption of different multivalent cations, i.e., Ni2+, poly-l-lysine (PLL), and spermidine (Spdn). DON adsorption is studied in phosphate-buffered saline (PBS) and pure water. In general, Ni2+ shows the worst performance with heavily deformed DONs. For 2D DON triangles, adsorption at PLL- and in particular Spdn-modified mica may outperform even Mg2+-mediated adsorption in terms of surface coverage, depending on the employed solution. For 3D six-helix bundles, less pronounced differences between the individual strategies are observed. Our results provide some general guidance for the immobilization of DONs at mica surfaces under Mg2+-free conditions and may aid future in situ AFM studies.

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“…In recent decades, aptamers have played critical roles in DDS because of their unique characteristics such as programmability, flexibility, and low toxicity. 3 For example, AS1411, a well-studied aptamer, can form a dimeric G-quadruplex structure to target high nucleolin-expressing cancer cells and thus has been frequently used as a tether for the drug nanocarrier. 4,5 Recently, AS1411 has aroused particular interests since it can effortlessly capture the G4-ligand, such as 5,10,15,20tetrakis(4-N-methylpyridiniumyl)porphyrin (TMPyP) which has been proved by our group.…”

Section: Introductionmentioning

confidence: 99%

VEGF aptamer/i-motif-grafted multi-functional SPION nanocarrier for chemotherapeutic/phototherapeutic synergistic research

Fu¹,

Lin

Liu³

et al. 2021

J Biomater Appl

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Chemotherapeutic agents and photosensitizers often suffer from poor tumor selectivity, high side toxicity, or low water solubility. To address these problems, various drug delivery systems (DDS) have been explored but most of them are toxic, difficult to synthesize, or of single function. In order to design a highly biocompatible, conveniently prepared, multi-functional drug delivery system, herein, an aptamer of vascular endothelial growth factor (VEGF) and a cytosine (C)-DNA fragment were grafted on the surface of superparamagnetic iron oxide nanoparticles (SPION), and then a chemotherapeutic agent daunomycin (DNM) and a photosensitizer 5, 10, 15, 20-tetra (phenyl-4-N-methyl-4-pyridyl) porphyrin (TMPyP) were self-assembled with the hybridized VEGF-based DNA structure. By loading DNM and TMPyP, the DDS displayed strong chemotherapeutic/phototherapeutic capability against cancer cells via mechanisms such as mitochondrial dysfunction and ROS elevation, which triggered the apoptosis of the tumor cells. The dual delivery of chemotherapeutical agents and photosensitizers with aptamer/C-rich DNA successfully integrated the functions of pH stimuli-responsive drug release and chemotherapeutic/phototherapeutic modalities into one single system and thus could be considered as an ideal drug delivery vehicle with great potential in clinic.

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Functionalization of Cellular Membranes with DNA Nanotechnology

Cited by 24 publications

References 86 publications

Application of Nucleic Acid Frameworks in the Construction of Nanostructures and Cascade Biocatalysts: Recent Progress and Perspective

Application of Nucleic Acid Frameworks in the Construction of Nanostructures and Cascade Biocatalysts: Recent Progress and Perspective

Magnesium-Free Immobilization of DNA Origami Nanostructures at Mica Surfaces for Atomic Force Microscopy

VEGF aptamer/i-motif-grafted multi-functional SPION nanocarrier for chemotherapeutic/phototherapeutic synergistic research

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