Functionalization of Fe3O4 magnetic nanoparticles with RGD peptide derivatives

Demin, A. M.; Vigorov, A. Yu.; Низова, И. А.; Уймин, М. А.; Щеголева, Н. Н.; Ермаков, А. Е.; Краснов, В. П.; Charushin, Valery N.

doi:10.1016/j.mencom.2013.12.006

Cited by 23 publications

(14 citation statements)

References 14 publications

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“…37,38 As a result, the MNPs containing amino groups on the surface (MNPs-NH 2 ; Figure 3 Figure 1A and B).…”

mentioning

confidence: 99%

3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by <em>Opisthorchis felineus</em>

Demin¹,

Pershina²,

Ivanov³

et al. 2016

IJN

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Purpose Liver fluke causes severe liver damage in an infected human. However, the infection often remains neglected due to the lack of pathognomonic signs. Nanoparticle-enhanced magnetic resonance imaging (MRI) offers a promising technique for detecting liver lesions induced by parasites. Materials and methods Surface modification of iron oxide nanoparticles produced by coprecipitation from a solution of Fe 3+ and Fe 2+ salts using 3-aminopropylsilane (APS) was carried out. The APS-modified nanoparticles were characterized by transmission electron microscopy, fourier transform infrared spectroscopy, and thermogravimetric analysis. Magnetic resonance properties of MNPs were investigated in vitro and in vivo. Results The amount of APS grafted on the surface of nanoparticles (0.60±0.06 mmol g −1 ) was calculated based on elemental analysis and infrared spectroscopy data. According to transmission electron microscopy data, there were no essential changes in the structure of nanoparticles during the modification. The APS-modified nanoparticles exhibit high magnetic properties; the calculated relaxivity r 2 was 271 mmol −1 s −1 . To obtain suspension with optimal hydrodynamic characteristics, amino groups on the surface of nanoparticles were converted into an ionic form with HCl. Cellular uptake of modified nanoparticles by rat hepatoma cells and human monocytes in vitro was 74.1±4.5 and 10.0±3.7 pg [Fe] per cell, respectively. Low cytotoxicity of the nanoparticles was confirmed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and Annexin V/7-aminoactinomycin D flow cytometry assays. For the first time, magnetic nanoparticles were applied for contrast-enhanced MRI of liver lesions induced by Opisthorchis felineus . Conclusion The synthesized APS-modified iron oxide nanoparticles showed high efficiency as an MRI contrast agent for the evaluation of opisthorchiasis-related liver damage.

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“…37,38 As a result, the MNPs containing amino groups on the surface (MNPs-NH 2 ; Figure 3 Figure 1A and B).…”

mentioning

confidence: 99%

3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by <em>Opisthorchis felineus</em>

Demin¹,

Pershina²,

Ivanov³

et al. 2016

IJN

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“…Magnetic materials are used routinely, e.g., in biology for magnetic separation of biomolecules and cells [4] or in chemistry for development of magnetic separation of catalysts [5]. A promising method for modifying iron-oxide nanoparticles (NP) consists of depositing an organic layer on MNP using functionalized alkoxysilanes, in particular, 3-aminopropyltrialkoxysilanes [6][7][8][9][10][11][12][13][14][15][16][17][18] that form an organosilicon layer with functional groups on the surface. This enables the NP to be modifi ed further.…”

Section: Introductionmentioning

confidence: 99%

“…IR spectroscopy is most often employed for a qualitative assessment of the degree of functionalization. As a rule, elemental analysis, inductively coupled plasma atomic-emission spectroscopy (AES), or thermogravimetric analysis (TGA) is used for a quantitative determination of the number of organic molecules on the MNP surface [6][7][8][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

“…Fluorescence or UV/vis spectrometry can determine the number of free molecules remaining in solution [6][7][8][9][15][16][17]. For example, MNP with surface 3-aminopropylsilane (APS) groups were modifi ed covalently with rhodamine B [15], N-difl uorenylmethoxycarbonyl-L-lysine [6], or S-naproxene [7] (production of amide derivatives) or p-nitrobenzaldehyde (Schiff base formation) [16,17] in order to determine the concentration of surface amines.…”

Section: Introductionmentioning

confidence: 99%

“…The APS-modifi ed MNP were prepared in analogy with previously described techniques [6][7][8]. IR spectroscopy confi rmed that APS was immobilized on the surface (Fig.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative Determination of 3-Aminopropylsilane on the Surface of FE3O4 Nanoparticles by Attenuated Total Reflection Infrared Spectroscopy

2014

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A technique for quantitative analysis of 3-aminopropylsilane on the surface of chemically modifi ed Fe 3 O 4 magnetic nanoparticles in the concentration range 0.32-3.03 mmol/g was developed using attenuated total refl ection infrared spectroscopy. The technique was based on the ratios of band areas corresponding to Fe-O vibrations of the nanoparticles and Si-O vibrations of the coating as a function of the Si mass fraction in the nanocomposite that was determined by inductively coupled plasma atomic-emission spectroscopy.Introduction. Magnetic nanoparticles (MNP), including those based on iron and its oxides (γ-Fe 2 O 3 and Fe 3 O 4 ), are currently being investigated for medical applications (targeted drug delivery, improved contrast in magnetic-resonance imaging, and hyperthermal treatment of tumors [1-3]). Magnetic materials are used routinely, e.g., in biology for magnetic separation of biomolecules and cells [4] or in chemistry for development of magnetic separation of catalysts [5]. A promising method for modifying iron-oxide nanoparticles (NP) consists of depositing an organic layer on MNP using functionalized alkoxysilanes, in particular, 3-aminopropyltrialkoxysilanes [6-18] that form an organosilicon layer with functional groups on the surface. This enables the NP to be modifi ed further. The ability to conjugate MNP to biomolecules or organic catalysts depends essentially on the surface concentration of functional groups. IR spectroscopy is most often employed for a qualitative assessment of the degree of functionalization. As a rule, elemental analysis, inductively coupled plasma atomic-emission spectroscopy (AES), or thermogravimetric analysis (TGA) is used for a quantitative determination of the number of organic molecules on the MNP surface [6-8, 10-14].The number of molecules immobilized on the MNP can be determined indirectly using sorption (desorption) or covalent binding of various organic chromophores to the surface groups. Fluorescence or UV/vis spectrometry can determine the number of free molecules remaining in solution [6][7][8][9][15][16][17]. For example, MNP with surface 3-aminopropylsilane (APS) groups were modifi ed covalently with rhodamine B [15], N-difl uorenylmethoxycarbonyl-L-lysine [6], or S-naproxene [7] (production of amide derivatives) or p-nitrobenzaldehyde (Schiff base formation) [16,17] in order to determine the concentration of surface amines. The degree of functionalization of the starting MNP was determined from the difference between the amounts of reagent added to the reaction mixture and unreacted reagent. Conductometric titration with HCl was used to determine the concentration of amines on the MNP surface [18]. The conductivity of the suspension began to rise rapidly after all amines were neutralized. The amount of consumed HCl and; therefore, the amount of amines could be calculated from this.The goal of the present work was to develop a technique for measuring the amount of APS from 0.320 to 3.06 mmol/g of MNP on the surface of chemically modifi ed Fe 3 O 4 MNP us...

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Synthesis of nanocomposite with a core—shell structure based on Fe3O4 magnetic nanoparticles and iron glycerolate

et al. 2019

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Functionalization of Fe3O4 magnetic nanoparticles with RGD peptide derivatives

Cited by 23 publications

References 14 publications

3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by <em>Opisthorchis felineus</em>

3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by <em>Opisthorchis felineus</em>

Quantitative Determination of 3-Aminopropylsilane on the Surface of FE3O4 Nanoparticles by Attenuated Total Reflection Infrared Spectroscopy

Synthesis of nanocomposite with a core—shell structure based on Fe3O4 magnetic nanoparticles and iron glycerolate

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