Functionalization of paramagnetic nanoparticles for protein immobilization and purification

Carneiro, Lara Aparecida Buffoni Campos; Ward, Richard J.

doi:10.1016/j.ab.2017.11.005

Cited by 26 publications

(9 citation statements)

References 21 publications

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“…The synthesis methodology of iron oxide nanoparticles (FeO NP) can occur from different materials, but many of the adverse effects are associated with toxicity, due to the presence of absorbed substances on the surface of the nanoparticles [9][10][11]; in these cases, some work uses new mechanisms such as 'nano zero valent' iron nanoparticles (nZVI) that are stored in nitrogen gas to avoid oxidation during the synthesis or drying [12][13][14]. On the contrary, an oxygenic atmosphere favors the generation of magnetic NP Fe (Fe 3 O 4 and Fe 2 O 3 ) which stand out for their high specific surface and absorbent capacity, and due to their magnetism they enable the extraction of particles through external magnetic fields [15,16] and nanoparticles with superparamagnetic properties [17,18].…”

Section: Introductionmentioning

confidence: 99%

Influence of the Alcoholic/Ethanolic Extract of Mangifera indica Residues on the Green Synthesis of FeO Nanoparticles and Their Application for the Remediation of Agricultural Soils

et al. 2021

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The green synthesis of iron oxide nanoparticles (FeO NP) has been investigated using the extract in absolute ethanolic and alcoholic solvents 96% from the peel of the mango fruit (Mangifera indica), thus evaluating the influence of the type of solvent on the extraction of reducing metabolites. A broad approach to characterization initially controlled by UV-vis spectrophotometry has been directed, the formation mechanism was evaluated by Fourier transform infrared spectroscopy (FTIR), the magnetic properties by characterization by Physical Property Measurement System (PPSM), in addition to a large number of techniques such as X-ray energy dispersive spectroscopy (EDS), X-ray diffraction (DRX), transmission electron microscopy (TEM/STEM), electron energy loss spectroscopy (EELS), and Z potential to confirm the formation of FeO NP. The results suggest better characteristics for FeO NP synthesized using 96% alcoholic solvent extract. The successful synthesis was directly proven in the removal of metals (Cr-VI, Cd, and Pb) as a potential alternative in the remediation of agricultural soils.

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Section: Introductionmentioning

confidence: 99%

Influence of the Alcoholic/Ethanolic Extract of Mangifera indica Residues on the Green Synthesis of FeO Nanoparticles and Their Application for the Remediation of Agricultural Soils

et al. 2021

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“…These properties include enormous surface to volume ratio which is too much important for immobilization point of view, large surface area, loading capacity and specifically magnetic/superparamagnetic property. Magnetic orsuperparamagnetic nanoparticles used as a useful carrier because MNPs contains magnetic properties, ease to separate under the external magnetic field [18]. Most commonly used MNPs are Fe3O4 and Maghemite due to very low toxicity levels.…”

mentioning

confidence: 99%

Biomedical uses of Enzymes Immobilized by Nanoparticles

Naqvi¹

2022

Nanomaterial-Supported Enzymes

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Immobilized enzymes are now a significant and appropriate area of modern technologies. Immobilization of enzymes on nanoparticles (NPs) especially magnetic nanoparticles (MNPs) not only increase the stability of the enzymes by protecting the active site but also facilitates the separation mode. Immobilized technology is considered effective in context of running cost to exercise immobilized enzymes technique. Nowadays, variety of magnetic nanoparticles are available such as chitin-chitosan magnetic nanoparticles, Fe3O4 magnetic nanoparticles, bacteriophages T4 capsid novozym-435 etc. which are quite fit for loading enzymes and to use fruitfully. The main focus in this piece of work is that how immobilized enzymes are helpful in different biomedical uses and what kind of enzymes and nanoparticles could be hyphenated to take advantage in health care sectors. Different method of enzymes immobilization will also be discussed in details including both physical methods and chemical methods of loading enzymes on nanoparticles.

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“…Em seguida as partículas magnéticas revestidas por quitosana são tratadas com glutaraldeído, devido sua ação como agente reticularizante. Para a imobilização via afinidade, pela coordenação do anel imidazólico das histidinas com o Ni 2+ , a preparação das nanopartículas prossegue com a adição de grupos amino pela incubação com AB-NTA e de grupos Ni 2+ pela incubação com NiCl 2 (CARNEIRO; WARD, 2018). No entanto, para a finalidade de imobilização via ligação covalente, as nanopartículas tratadas até a etapa de reticularização com o glutaraldeído são capazes de promover essas ligações.…”

Section: Resultsunclassified

“…Para este trabalho, foram empregadas as nanopartículas magnéticas produzidas no Laboratório de Bioquímica e Biofísica de Proteínas (LBBP) do Departamento de Química da FFCLRP, Universidade de São Paulo. Esse nanomaterial foi descrito recentemente por Carneiro e Ward ( 2016), com uma patente registrada sob o n° BR 102016021095-0, e já foi aplicado com sucesso na imobilização de uma enzima β-galactosidase (CARNEIRO; WARD, 2018). O nanocomposto revestido por quitosana e funcionalizado por glutaraldeído, ácido N-(5-amino-1carboxi-pentil) iminodiacético (AB-NTA) e níquel, mostrou-se estável em solução e adequado para imobilizar enzimas, bem como ser aplicado na purificação de proteínas recombinantes que carregam His-tag, substituindo portanto matrizes de afinidade.…”

Section: Imobilização E Engenharia De Proteínasunclassified

“…O nanocomposto revestido por quitosana e funcionalizado por glutaraldeído, ácido N-(5-amino-1carboxi-pentil) iminodiacético (AB-NTA) e níquel, mostrou-se estável em solução e adequado para imobilizar enzimas, bem como ser aplicado na purificação de proteínas recombinantes que carregam His-tag, substituindo portanto matrizes de afinidade. Ainda, o nanomaterial demonstrou grande utilidade de reuso visto que a enzima imobilizada reteve ainda 80% da atividade após nove ciclos de lavagens (CARNEIRO; WARD, 2018).…”

Section: Imobilização E Engenharia De Proteínasunclassified

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Um sistema biomimético nanoestruturado para a hidrólise enzimática de Xiloglucano

Soares¹

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Lignocellulosic materials are abundant agroindustrial byproducts and constitute a renewable carbon source that can be used for biofuel production, such as second-generation bioethanol. Xyloglucan is a hemicellulose and one of the most abundant polysaccharides in lignocellulose and it is entirely hydrolyzed by the combined action of at least four different enzymes. The approach of protein immobilization on nanomaterials offers the possibility of colocalisation of different enzymes on structures designed to mimic biological reactions, and even entire metabolic pathways. In addition, the use of nanomaterials in enzyme immobilization may increase the synergistic effect due to the proximity effect between the catalytic centers. In this work, the immobilization of four enzymes for xyloglucan depolymerization was studied, with the purpose of developing biomimetic nanostructures. The main objectives were: the expression of the enzymes -xyloglucan specific endo-β-1,4-glucanase, β-galactosidase, α-xylosidase and β-glucosidase, and their immobilization on magnetic nanoparticles and consequent characterization of the activity of these nanostructures against xyloglucan. The heterologously expressed enzymes were covalently immobilized, individually and in combination, on ferromagnetic iron oxide (Fe 3 O 4 ) nanoparticles coated with chitosan and cross-linked with glutaraldehyde. These hybrid nanostructures were then tested for their catalytic capacity against synthetic substrates and xyloglucan extracted from Jatobá (Hymenaea courbaril) seeds. Enzymatic characterizations demonstrated differences for optimal pH and temperatures determined between free and immobilized enzymes. All enzymes remained active after co-immobilization on the nanomaterial, however specific activity was reduced for all enzymes after immobilization. The optimum temperature of immobilized α-xylosidase YicI increased by ten degrees compared to the free enzyme. Binding efficiency on the particles ranged from 5 to 92% between enzymes. The enzyme β-galactosidase Bga1 demonstrated the best immobilization efficiency as well as the highest reuse capacity, maintaining up to 50% of activity after ten cycles. In addition, assays with different enzymatic proportions were performed to optimize xyloglucan hydrolysis by the four enzyme-containing nanosystems. The capacity of xyloglucan hydrolysis by the nanosystems was determined by measuring glucose released after the reactions and by mass spectrometry of the reaction products. Mass spectrometry results indicated the presence of the xylose and glucose/galactose monosaccharides in the xyloglucan hydrolysis products, showing the depolymerization activity of the hybrid nanostructures containing the four enzymes.

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Functionalization of paramagnetic nanoparticles for protein immobilization and purification

Cited by 26 publications

References 21 publications

Influence of the Alcoholic/Ethanolic Extract of Mangifera indica Residues on the Green Synthesis of FeO Nanoparticles and Their Application for the Remediation of Agricultural Soils

Influence of the Alcoholic/Ethanolic Extract of Mangifera indica Residues on the Green Synthesis of FeO Nanoparticles and Their Application for the Remediation of Agricultural Soils

Biomedical uses of Enzymes Immobilized by Nanoparticles

Um sistema biomimético nanoestruturado para a hidrólise enzimática de Xiloglucano

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