Functionalization of single-walled carbon nanotubes enables efficient intracellular delivery of siRNA targeting MDM2 to inhibit breast cancer cells growth

Chen, Hailong; Ma, Xiaochun; Li, Zhi; Shi, Qi; Zheng, Wenyun; Wang, Ping

doi:10.1016/j.biopha.2011.12.005

Cited by 76 publications

(37 citation statements)

References 22 publications

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“…The 2Ј-O-methyl interfering RNAs directly against HBXIP 3Ј-UTR, p53, MDM2, and p300 and the control siRNAs were purchased from the Riobio Co. (Guangzhou, China). The sequences of siRNAs for MDM2, HBXIP, p53, and p300 were described previously (16,(31)(32)(33)(34).…”

Section: Methodsmentioning

confidence: 99%

The Oncoprotein HBXIP Modulates the Feedback Loop of MDM2/p53 to Enhance the Growth of Breast Cancer

Liu

Wang

et al. 2015

Journal of Biological Chemistry

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Background: MDM2 and p53 form a negative feedback loop in regulation. Results: HBXIP up-regulates MDM2 by recruiting p300 to activate transcription factor p53. Conclusion: HBXIP is involved in the MDM2/p53 feedback loop to promote the growth of breast cancer. Significance: Our finding provides a new insight into the mechanism of MDM2/p53 circuit involved in HBXIP in the development of breast cancer.

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Section: Methodsmentioning

confidence: 99%

The Oncoprotein HBXIP Modulates the Feedback Loop of MDM2/p53 to Enhance the Growth of Breast Cancer

Liu

Wang

et al. 2015

Journal of Biological Chemistry

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“…Through their functionalization, fullerenes, graphenes, and CNTs are capable of carrying imaging agents or chemotherapeutic drugs for diagnosing and treating breast cancer [20,67,68]. This confers these systems an additional advantage and allows them to contribute to cancer detection and also to the targeted and controlled administration of drugs.…”

Section: Contrast Agentmentioning

confidence: 99%

“…This induces cytotoxic effects that lead to an irreversible photodamage of cancer cells [71][72][73][74]77]. In this regard, carbon nanomaterials have been used successfully to cause thermal ablation of solid tumors in breast cancer studies [16,68,[74][75][76][79][80][81][82]. Ogbodu et al [74] reported for the first time the synthesis, photophysical properties, and PDT activity of ZnMCPPc-spermine-SWCNT (zinc monocarboxy phenoxy phthalocyanine upon conjugation to spermidine, as a targeting molecule, and then adsorbed onto SWCNT) on breast cancer cells (MCF-7).…”

Section: Photodynamicmentioning

confidence: 99%

Carbon Nanomaterials for Breast Cancer Treatment

Casais-Molina

Cab

Canto

et al. 2018

Journal of Nanomaterials

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Currently, breast cancer is considered as a health problem worldwide. Furthermore, current treatments neither are capable of stopping its propagation and/or recurrence nor are specific for cancer cells. Therefore, side effects on healthy tissues and cells are common. An increase in the efficiency of treatments, along with a reduction in their toxicity, is desirable to improve the life quality of patients affected by breast cancer. Nanotechnology offers new alternatives for the design and synthesis of nanomaterials that can be used in the identification, diagnosis, and treatment of cancer and has now become a very promising tool for its use against this disease. Among the wide variety of nanomaterials, the scientific community is particularly interested in carbon nanomaterials (fullerenes, nanotubes, and graphene) due to their physical properties, versatile chemical functionalization, and biocompatibility. Recent scientific evidence shows the potential uses of carbon nanomaterials as therapeutic agents, systems for selective and controlled drug release, and contrast agents for diagnosing and locating tumors. This generates new possibilities for the development of innovative systems to treat breast cancer and can be used to detect this disease at much earlier stages. Thus, applications of carbon nanomaterials in breast cancer treatment are discussed in this article.

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“…Wang et al reported that after SWNT were chemically functionalized with PEI, the transfection rate of SWNT-PEI/siRNA was approximately 87% . Based on the noncovalent functionalization of SWNT with DSPE-PEG2000- amino, when the chemical conjugation of thiol-modified siRNA to DSPE-PEG-amine (PEI-SA/CNT/siRNA) was used, the inhibition ratio of tumor cell reached 44.53% (Chen et al, 2012). On the premise of similar transfection and silencing efficiency, as well as cell growth inhibition, trivial steps, time-consuming processes and the risk and toxicity of chemicals are avoided in the protocol we use to prepare our vector.…”

Section: Delivery Ability Rnai Efficiency and Tumor Cell Inhibitionmentioning

confidence: 99%

“…Among the various attempts to deliver siRNA using SWNT, the conjugation of siRNA to SWNT through chemical reactions, such as the chemical conjugation of thiol-modified siRNA to non-covalently functionalized SWNT or the conjugation of siRNA to chemically functionalized SWNT, is complicated and time consuming (Zhang et al, 2006;Liu et al, 2007;Krajcik et al, 2008;Wang et al, 2008;Liu et al, 2009;Podesta et al, 2009;Al-Jamal et al, 2010;Al-Jamal et al, 2011;Varkouhi et al, 2011;Chen et al, 2012;Battigelli et al, 2013;Wang et al, 2013). However, nanoparticle preparation through electrostatic interactions between the negatively charged nucleic acids and the non-covalently functionalized cationic SWNT may have more potential for siRNA delivery due to the ease and convenience of this technique.…”

Section: Introductionmentioning

confidence: 99%

DOTAP functionalizing single-walled carbon nanotubes as non-viral vectors for efficient intracellular siRNA delivery

Hao

Wang

et al. 2014

Drug Delivery

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Context: Functionalized single-walled carbon nanotubes (SWNT) with 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) were used as novel and more convenient carriers of small interfering RNA (siRNA).Objective: To utilize the unique capability of SWNT to be easily modified by functional groups and readily internalized by mammalian cells to bind, condense, stabilize siRNA and enhance its transfection efficiency. Methods: After SWNT were non-covalently functionalized by cationic DOTAP (SWNT-DOTAP), siRNA interacted with SWNT-DOTAP via static electricity (SWNT-DOTAP/siRNA). Subsequently, the size, zeta potential and morphology of SWNT-DOTAP/siRNA were analyzed. The optimal compression ratio and stability of siRNA were assessed by agarose gel electrophoresis. Furthermore, in prostate carcinoma PC-3 cells, RT-PCR, flow cytometry and sulforhodamine B assays were used to evaluate the silencing activity, transfection efficiency and cell proliferation, respectively. Results and discussion: The characteristics of SWNT-DOTAP, i.e. an average size of 194.49 nm, a zeta potential of 45.16 mV and lower cytotoxicity than Lipofectamine 2000, indicated that this vector was suitable for siRNA delivery. Moreover, after interaction with SWNT-DOTAP, siRNA of human telomerase reverse transcriptase was bound, condensed and stabilized. In PC-3 cells, SWNT-DOTAP/siRNA exhibited 82.6% silencing activity and 92% transfection efficiency. Furthermore, the complexes inhibited cell proliferation by 42.1%. Conclusion: SWNT-DOTAP may be a promising siRNA delivery vector for gene-based therapeutic applications in cancer.

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Functionalization of single-walled carbon nanotubes enables efficient intracellular delivery of siRNA targeting MDM2 to inhibit breast cancer cells growth

Cited by 76 publications

References 22 publications

The Oncoprotein HBXIP Modulates the Feedback Loop of MDM2/p53 to Enhance the Growth of Breast Cancer

The Oncoprotein HBXIP Modulates the Feedback Loop of MDM2/p53 to Enhance the Growth of Breast Cancer

Carbon Nanomaterials for Breast Cancer Treatment

DOTAP functionalizing single-walled carbon nanotubes as non-viral vectors for efficient intracellular siRNA delivery

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