Functionalized Magnetic Nanoparticles for the Detection and Quantitative Analysis of Cell Surface Antigen

Shahbazi‐Gahrouei, Daryoush; Abdolahi, Mohammad; Zarkesh-Esfahani, Hamid; Laurent, Sophie; Sermeus, Corine; Gruettner, Cordula

doi:10.1155/2013/349408

Cited by 22 publications

(20 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Technological developments such as intensity-modulated radiation therapy (IMRT) and improved target localization such as using nanoparticles as a targeted therapy have been combined with radiation biology to generate enthusiasm for hypofractionated regimens (4,5).…”

Section: Contextmentioning

confidence: 99%

“…If it is too fast (e.g. multiple fractions per day), the therapy dose does not allow for rapidly dividing cells such as mucosal cells to repopulate themselves, which have been killed by the radiation (5,28,29). A technique, which could be used for a hypofractionated regimen is to convert the dose received by the mucosal cells that could be equal to the tumor prescribed dose, or to a 2 Gy equivalent dose (EQD2-Gy), applying an α/β = 10 Gy value and afterward see if it is within the 'safe' limit for the total treatment time (30).…”

Section: Radical Alteration Of Hypofractionation Criterionmentioning

confidence: 99%

“…Using a Poisson distribution model, Equations 5 calculate tumor control probability (TCP) and normal tissue complication probability (NTCP) values: (5) T CP = e −n.SF (33). And applying NTCP for the rectum can be a great help for the decision-making process in the treatment planning.…”

Section: Contrast Between Hypofractionation and Standard Fractionationmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Hypofractionation on Prostate Cancer Radiotherapy

Arabpour

Shahbazi‐Gahrouei

2017

Int J Cancer Manag

Self Cite

View full text Add to dashboard Cite

Context: Prior research over the past few decades has shown that the hypofractionated external beam radiation therapy could be a valuable method with an outstanding potential to treat the prostate cancer. The alpha-beta ratio (α/β) is an indication of the fractionation sensitivity of a particular cell type. Values of α/β ≥ 10 Gy are responsible for early-responding normal tissues and most tumors, while values of α/β < 5 Gy are for late-responding normal tissues. The α/β ratio could be raised by either a local tumor control increase or a decrease of normal tissue late effects through the application of an unconventional fractionation schedules. Evidence Acquisition: This review article aimed to investigation the details of radiobiological and clinical trials which are reported to highlight the characteristics of hypofractionation for the prostate cancer treatment based on the investigation of clinical results from hypofractionated treatments. Results:With the sufficient radiobiological and physiological research studies, it may be claimed that if α/β value for prostate cancer is less than the adjacent surrounding organs at risk and by applying fewer and larger fractions; a better therapeutic ratio can be achieved for the patient. Conclusions: Radiobiological modeling can play a major role in the design of new protocols. Also, one should be aware that in modeling the hypofractionated treatment, normal tissue iso-effects are significantly important and some assumptions needed to be made regarding α/β values for the critical normal tissue complication endpoint.

show abstract

Section: Contextmentioning

confidence: 99%

Section: Radical Alteration Of Hypofractionation Criterionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Hypofractionation on Prostate Cancer Radiotherapy

Arabpour

Shahbazi‐Gahrouei

2017

Int J Cancer Manag

Self Cite

View full text Add to dashboard Cite

show abstract

“…Sulfo-SMCC (4-(N-Maleimidomethyl)cyclohexane-1-carboxylic acid 3-sulfo-N-hydroxysuccinimide ester) cross-linker was first added to SPIO nanoparticles with CLD-NH 2 (aminated cross-linked dextran) surface to introduce maleimide groups as previously described. 18 Briefly, sulfo-SMCC solution (100 µL of 14.35 µM) in DMSO (dimethyl sulfoxide) was added to 5 mg of iron oxide nanoparticles in PBS-EDTA buffer under gentle shaking for 1 hour at room temperature. After incubation, the suspension was washed with PBS-EDTA buffer with PD (protein desalting)-10 sizeexclusion columns to remove unreacted sulfo-SMCC.…”

Section: Antibody-conjugated Spio Functionalizationmentioning

confidence: 99%

MR imaging and targeting of a specific alveolar macrophage subpopulation in LPS-induced COPD animal model using antibody-conjugated magnetic nanoparticles

Faraj

Shaik

Afzal

et al. 2014

IJN

View full text Add to dashboard Cite

Purpose Targeting and noninvasive imaging of a specific alveolar macrophage subpopulation in the lung has revealed the importance for early and better diagnosis and therapy of chronic obstructive pulmonary disease (COPD). In this study, the in vivo effect of pulmonary administration of iron oxide nanoparticles on the polarization profile of macrophages was assessed, and a noninvasive free-breathing magnetic resonance imaging (MRI) protocol coupled with the use of biocompatible antibody-conjugated superparamagnetic iron oxide (SPIO) nanoparticles was developed to enable specific targeting and imaging of a particular macrophage subpopulation in lipopolysaccharide-induced COPD mice model. Materials and methods Enzyme-linked immunosorbent assay, Real-time polymerase chain reaction, and flow cytometry analysis were performed to assess the biocompatibility of PEGylated dextran-coated SPIO nanoparticles. Specific biomarkers for M1 and M2 macrophages subsets were selected for conjugation with magnetic nanoparticles. MRI protocol using ultra-short echo time sequence was optimized to enable simultaneous detection of inflammation progress in the lung and detection of macrophages subsets. Flow cytometry and immunohistochemistry analysis were finally performed to confirm MRI readouts and to characterize the polarization profile of targeted macrophages. Results The tested SPIO nanoparticles, under the current experimental conditions, were found to be biocompatible for lung administration in preclinical settings. Cluster of differentiation (CD)86- and CD206-conjugated magnetic nanoparticles enabled successful noninvasive detection of M1 and M2 macrophage subpopulations, respectively, and were found to co-localize with inflammatory regions induced by lipopolysaccharide challenge. No variation in the polarization profile of targeted macrophages was observed, even though a continuum switch in their polarization might occur. However, further confirmatory studies are required to conclusively establish this observation. Conclusion Coupling of magnetic iron oxide nanoparticles with a specific antibody targeted to a particular macrophage subpopulation could offer a promising strategy for an early and better diagnosis of pulmonary inflammatory diseases using noninvasive MRI.

show abstract

“…MRI uses contrast agents that are materials that enhance the image contrast. MRI contrast agents modify relaxation time of the atomic nucleus in tissue Electronic supplementary material The online version of this article (doi: 10.1007/s11307-015-0841-9) contains supplementary material, which is available to authorized users. hence providing a more intense signal [1].…”

Section: Introductionmentioning

confidence: 99%