In Vitro Evaluation of Gd3+-Anionic Linear Globular Dendrimer-Monoclonal Antibody: Potential Magnetic Resonance Imaging Contrast Agents for Prostate Cancer Cell Imaging

Mirzaei, Mehdi; Mehravi, Bita; Ardestani, Mehdi Shafiee; Ziaee, Seyed Amir Mohsen; Pourghasem, Peyman

doi:10.1007/s11307-015-0841-9

Cited by 7 publications

(3 citation statements)

References 19 publications

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“…ALGDG2 was introduced for the first time by Namazi and Adeli (2005) 19 . Easy synthesis, low molecular weight, well-known functional groups on the surface of the spherical particle, monodispersity, high purity and hydrophilicity, increased permeability to cancerous cells and most importantly low toxicity with low immune system stimulation when injected locally 20 for its biocompatible PEGylated core and biodegradable sidelong citric acid groups made ALGDG2 a suitable carrier for a range of drugs and agents, such as anticancer 21 , antiviral 22 , antibacterial drugs 23 and imaging agents 24 .…”

Section: Introductionmentioning

confidence: 99%

AS1411 Aptamer-Anionic Linear Globular Dendrimer G2-Iohexol Selective Nano-Theranostics

Mohammadzadeh

Cohan

Ghoreishi

et al. 2017

Sci Rep

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Molecular theranostics is of the utmost interest for diagnosis as well as treatment of different malignancies. In the present study, anionic linear globular dendrimer G2 is employed as a suitable carrier for delivery and AS1411 aptamer is exploited as the targeting agent to carry Iohexol specifically to the human breast cancer cells (MCF-7). Dendrimer G2 was prepared and conjugation of dendrimer and aptamer was carried out thereafter. Based on the data yielded by AFM, morphology of smooth and spherical non-targeted dendrimer changed to the rough aspherical shape when it conjugated. Then, conjugation was confirmed using DLS, ELS and SLS methods. Toxicity on nucleolin positive MCF-7 cells and nucleolin negative HEK-293 cells was assessed by XTT and apoptosis/necrosis assays. In vitro uptake was determined using DAPI-FITC staining and ICP-MS methods. In vivo studies including in vivo CT imaging, pathology and blood tests were done to confirm the imaging ability, bio-safety and targeted nature of the Nano-Theranostics in vivo. In a nutshell, the prepared construction showed promising effects upon decreasing the toxicity of Iohexol on normal cells and accumulation of it in the cancer tumors as well as reducing the number of cancer cells.

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Section: Introductionmentioning

confidence: 99%

AS1411 Aptamer-Anionic Linear Globular Dendrimer G2-Iohexol Selective Nano-Theranostics

Mohammadzadeh

Cohan

Ghoreishi

et al. 2017

Sci Rep

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“…This dendrimer consists of a biocompatible PEGylated core and biodegradable peripheral citric acid groups which its monomeric units are the chemical intermediates or products of metabolic pathways (such as, Krebs cycle) [27]. These compounds can be metabolised and removed by the cellular processes or used as cells' energy source step by step.…”

Section: Discussionmentioning

confidence: 99%

PEG‐Citrate dendrimer second generation: is this a good carrier for imaging agents In Vitro and In Vivo ?

Mohammadzadeh

Ardestani

Mortazavi‐Derazkola

et al. 2019

IET nanobiotechnol.

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While cancer is the leading cause of human's deaths worldwide, finding an imaging agent which can detect cancer tumours is needed for cancer diagnosis. In the present study, PEG-citrate dendrimer-G 2 was used as a nano-carrier of FITC dye and Iohexol to help passive targeting and uptake of both imaging agents in cancer cells/tumour in vitro and in vivo. Dendrimer was synthesisedand the product characterised using LC-MS, FT-IR, DLS, ELS, AFM, and 1 HNMR. After FITC loading into dendrimer, MTT was performed to determine the cytotoxicity of formulation on HEK-293 and MCF-7 cells. In vitro imaging using dendrimer-FITC was done via fluorescent microscope thereafter. Moreover, CT imaging using Iohexol was employed to show the targeting nature and ability of the complex to use as imaging agent in vivo. Data yielded in this study corroborate the notion that the promised dendrimer was synthesised properly and had no toxicity along with FITC on normal cell. Furthermore, CT and fluorescent images showed the targeting nature and imaging ability of Iohexol/FITC loaded dendrimer in vitro and in vivo. Overall, results showed promising characteristics of the novel complexes using dendrimer-G 2 both in vitro and in vivo.

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“…Another contribution to the topic has been conducted by Mohammadzadeh et al [54] as they developed a selective nano-theranostic system composed of AS1411-functionalized anionic linear globular dendrimer G2 (ALGDG2) for the specific delivery of Iohexol to breast cancer cells. ALGDG2 is generally considered as an ideal carrier for a broad spectrum of anticancer, antiviral, and imaging agents due to its low molecular weight, low toxicity, immunocompatibility, biodegradability (because of its sidelong citric acid groups), high purity and hydrophilicity, monodispersity, favorable permeability to cancer cells, and possession of various wellknown functional groups on the spherical particles' surface [55][56][57][58]. Taken together, these nano constructs exhibited a considerable potential for reducing the number of cancer cells by maximizing the accumulation of iohexol in the tumors while they minimized the toxicity of Iohexol on normal cells [54].…”

Section: Chemotherapeutic Deliverymentioning

confidence: 99%

AS1411-functionalized delivery nanosystems for targeted cancer therapy

Kozani¹,

Kozani²,

Malik³

2021

Exploration of Medicine

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Nucleolin (NCL) is a multifunctional nucleolar phosphoprotein harboring critical roles in cells such as cell proliferation, survival, and growth. The dysregulation and overexpression of NCL are related to various pathologic and oncological indications. These characteristics of NCL make it an ideal target for the treatment of various cancers. AS1411 is a synthetic quadruplex-forming nuclease-resistant DNA oligonucleotide aptamer which shows a considerably high affinity for NCL, therefore, being capable of inducing growth inhibition in a variety of tumor cells. The high affinity and specificity of AS1411 towards NCL make it a suitable targeting tool, which can be used for the functionalization of therapeutic payload-delivery nanosystems to selectively target tumor cells. This review explores the advances in NCL-targeting cancer therapy through AS1411-functionalized delivery nanosystems for the selective delivery of a broad spectrum of therapeutic agents.

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In Vitro Evaluation of Gd3+-Anionic Linear Globular Dendrimer-Monoclonal Antibody: Potential Magnetic Resonance Imaging Contrast Agents for Prostate Cancer Cell Imaging

Abstract: These results showed that Gd(3+)-ALGDG2-C595 is a potential prostate molecular imaging agent and could be considered as an ideal functional nanoprobe. Additionally, further investigations by clinical trials are in the pipeline.

Cited by 7 publications

References 19 publications

AS1411 Aptamer-Anionic Linear Globular Dendrimer G2-Iohexol Selective Nano-Theranostics

AS1411 Aptamer-Anionic Linear Globular Dendrimer G2-Iohexol Selective Nano-Theranostics

PEG‐Citrate dendrimer second generation: is this a good carrier for imaging agents In Vitro and In Vivo ?

AS1411-functionalized delivery nanosystems for targeted cancer therapy

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