Functions of phytosterols in seed development of upland cotton (Gossypium hirsutum L.)

Suo, Xiaodong; Xu, Fan; Tan, Kunling; Huang, Li; Bao, Chaoya; Luo, Ming

doi:10.1016/j.indcrop.2021.113802

Cited by 9 publications

(7 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interconversions between β-sitosterol and stigmasterol are associated with tolerance to biotic and abiotic stresses by modifying the permeability of plant cell membranes [ 56 , 57 , 58 ]. In cottonseed, this gene is induced during plant development together with other genes that participate in the plant sterol pathway [ 59 ]. Moreover, stigmasterol has several benefits for human health, and the overexpression CYP710A1 generated a 2.6× increase in this compound in tomato fruit [ 37 ].…”

Section: Resultsmentioning

confidence: 99%

“…The SAHH gene is important for lignin biosynthesis and is necessary for monolignol methylation [ 40 ]. ACS1 and CYP90A1 are involved in plant hormone biosynthesis and induce germination, while CYP710A1 is related to plant sterols, which are responsible for membrane stability [ 47 , 51 , 59 ]. The ACSF3 gene acts in lipid metabolism and is of great importance for the synthesis of flavonoids and anthocyanin, serving as a substrate for the CHS enzyme [ 62 , 63 ].…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Transcriptional Profile of Soybean Seeds with Contrasting Seed Coat Color

Kafer

Molinari²,

Henning

et al. 2023

Plants

View full text Add to dashboard Cite

Soybean is the primary source of vegetable protein and is used for various purposes, mainly to feed animals. This crop can have diverse seed coat colors, varying from yellow, black, brown, and green to bicolor. Black seed coat cultivars have already been assigned as favorable for both seed and grain production. Thus, this work aimed to identify genes associated with soybean seed quality by comparing the transcriptomes of soybean seeds with contrasting seed coat colors. The results from RNA-seq analyses were validated with real-time PCR using the cultivar BRS 715A (black seed coat) and the cultivars BRS 413 RR and DM 6563 IPRO (yellow seed coat). We found 318 genes differentially expressed in all cultivars (freshly harvested seeds and seeds stored in cold chamber). From the in silico analysis of the transcriptomes, the following genes were selected and validated with RT-qPCR: ACS1, ACSF3, CYP90A1, CYP710A1, HCT, CBL, and SAHH. These genes are genes induced in the black seed coat cultivar and are part of pathways responsible for ethylene, lipid, brassinosteroid, lignin, and sulfur amino acid biosynthesis. The BRSMG 715A gene has almost 4times more lignin than the yellow seed coat cultivars. These attributes are related to the BRSMG 715A cultivar’s higher seed quality, which translates to more longevity and resistance to moisture and mechanical damage. Future silencing studies may evaluate the knockout of these genes to better understand the biology of soybean seeds with black seed coat.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Transcriptional Profile of Soybean Seeds with Contrasting Seed Coat Color

Kafer

Molinari²,

Henning

et al. 2023

Plants

View full text Add to dashboard Cite

show abstract

“…In plants, phytosterols are essential membrane compounds that stabilize and alter the biophysical properties of membranes and induce plant defense responses against various biotic and abiotic stresses. , In addition, they regulate biological processes, such as seed development, signal transduction, cell division, and embryo growth, thereby reinforcing the membrane and forming lipid rafts. − In humans, phytosterols reduce the level of total serum cholesterol and low-density lipoprotein cholesterol (LDL) in patients with dyslipidemia . Among phytosterols, β-sitosterol exhibits anti-atherogenic and anticancer effects on macrophage foam cells through the inhibition of macrophage cholesterol biosynthesis and prevention of carcinogenesis resulting from oxidative stress by increasing the level of the antioxidant enzyme glutathione peroxidase. , Stigmasterol reduces cell proliferation and tumor size and elicits anti-inflammatory effects by inhibiting the expression of the inflammatory mediator PGE 2 in mouse chondrocytes and human osteoarthritis (OA) chondrocytes. , Campesterol is a potent angiogenesis inhibitor used for the prevention or treatment of angiogenesis-related diseases .…”

Section: Discussionmentioning

confidence: 99%

Melatonin Treatment Enhances the Growth and Productivity of Useful Metabolites in the In Vitro Culture ofSpirodela polyrhiza

Ryu

Noh

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

Spirodela polyrhiza (Araceae family) is a duckweed species that serves as a potential resource for feed, food, bioremediation, and pharmaceutical applications. In this study, we assessed the effects of different concentrations of melatonin (0, 0.1, 1, and 10 μM) on the growth of S. polyrhiza during in vitro culture and the metabolic profiles and productivities of useful metabolites using gas chromatography–mass spectrometry coupled with multivariable statistical analysis. We found that exogenous melatonin significantly improved the total dry weight and altered the metabolic profiles of S. polyrhiza cultures. Melatonin significantly enhanced the cellular production of useful metabolites, such as γ-aminobutyric acid, dopamine, threonine, valine, and phytosterols. The volumetric productivities (mg/L) of γ-aminobutyric acid, dopamine, campesterol, β-sitosterol, and stigmasterol were the highest in the presence of 10 μM melatonin on day 12. Moreover, the productivities of ascorbic acid and serotonin were the highest in the presence of 1 μM melatonin on day 12. Therefore, melatonin could be used to enhance the production of biomass and useful metabolites during large-scale S. polyrhiza cultivation in cosmetic, food/feed, and pharmaceutical industries.

show abstract

“…Phytosterols have structural and functional roles in plant growth and development [ 49 , 50 ], and its role in numerous physiological processes is well established, such as cell division, cell elongation, vascular element development, and tolerance to abiotic and/or biotic stress [ 50 , 51 , 52 ]. Phytosterols also play vital roles in ovule growth and fiber elongation in cotton [ 41 , 53 , 54 , 55 ]. However, the role of sterols in the differentiation and initiation of fiber cell is still unclear.…”

Section: Discussionmentioning

confidence: 99%

Comparative Metabolomics Analysis Reveals Sterols and Sphingolipids Play a Role in Cotton Fiber Cell Initiation

Wang

Meng

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

Cotton fiber is a seed trichome that protrudes from the outer epidermis of cotton ovule on the day of anthesis (0 day past anthesis, 0 DPA). The initial number and timing of fiber cells are closely related to fiber yield and quality. However, the mechanism underlying fiber initiation is still unclear. Here, we detected and compared the contents and compositions of sphingolipids and sterols in 0 DPA ovules of Xuzhou142 lintless-fuzzless mutants (Xufl) and Xinxiangxiaoji lintless-fuzzless mutants (Xinfl) and upland cotton wild-type Xuzhou142 (XuFL). Nine classes of sphingolipids and sixty-six sphingolipid molecular species were detected in wild-type and mutants. Compared with the wild type, the contents of Sphingosine-1-phosphate (S1P), Sphingosine (Sph), Glucosylceramide (GluCer), and Glycosyl-inositol-phospho-ceramides (GIPC) were decreased in the mutants, while the contents of Ceramide (Cer) were increased. Detail, the contents of two Cer molecular species, d18:1/22:0 and d18:1/24:0, and two Phyto-Cer molecular species, t18:0/22:0 and t18:0/h22:1 were significantly increased, while the contents of all GluCer and GIPC molecular species were decreased. Consistent with this result, the expression levels of seven genes involved in GluCer and GIPC synthesis were decreased in the mutants. Furthermore, exogenous application of a specific inhibitor of GluCer synthase, PDMP (1-phenyl-2-decanoylamino-3-morpholino-1-propanol), in ovule culture system, significantly inhibited the initiation of cotton fiber cells. In addition, five sterols and four sterol esters were detected in wild-type and mutant ovules. Compared with the wild type, the contents of total sterol were not significantly changed. While the contents of stigmasterol and campesterol were significantly increased, the contents of cholesterol were significantly decreased, and the contents of total sterol esters were significantly increased. In particular, the contents of campesterol esters and stigmasterol esters increased significantly in the two mutants. Consistently, the expression levels of some sterol synthase genes and sterol ester synthase genes were also changed in the two mutants. These results suggested that sphingolipids and sterols might have some roles in the initiation of fiber cells. Our results provided a novel insight into the regulatory mechanism of fiber cell initiation.

show abstract

Functions of phytosterols in seed development of upland cotton (Gossypium hirsutum L.)

Cited by 9 publications

References 46 publications

Transcriptional Profile of Soybean Seeds with Contrasting Seed Coat Color

Transcriptional Profile of Soybean Seeds with Contrasting Seed Coat Color

Melatonin Treatment Enhances the Growth and Productivity of Useful Metabolites in the In Vitro Culture ofSpirodela polyrhiza

Comparative Metabolomics Analysis Reveals Sterols and Sphingolipids Play a Role in Cotton Fiber Cell Initiation

Contact Info

Product

Resources

About