Fundamental Relationships of Chromatography

Survey and Trends in the Preparation of Chemically Bonded Silica Phases for Liquid Chromatographic Analysis

“…Apparently, the molecules of modifier create an active complex, with the surface silanols and also bind the released reaction by-products. Consequently, a packing with dense coverage is created [1,51]. According to Buszewski et al [8,18], changing both the nature and the concentration of the activator permits preparation of packings with controlled and homogeneous coverage.…”

Analysis of Solanum alkaloids using internal standardization and capillary gas chromatography

“…Chromatographic conditions were optimized on the basis of the resolution of tomatidine and acetylleptinidine, since these two alkaloids were the most difficult to separate. These conditions provided nearbaseline resolution of tomatidine and acetylleptinidine (R = 1.4; Table I) with an analysis time of less than 20 min (Figure 4); an Rvalue of 1.5 constitutes baseline separation (Poole and Schuette, 1984). Thus, these conditions provided a good compromise between resolution and analysis time.…”