Fundamental Studies on Physiological and Pharmacological Actions of L-Ascorbate 2-Sulfate. V. On the Hypolipidemic and Anti Atherosclerotic Effects of L-Ascorbate 2-Sulfate in Rabbits

Abstract

The area rate of atheromatous plaque in aorta was less in specimens from both groups of AAS than in those from control I. Pathological changes in intima and media of aorta were milder in specimens from both groups of AAS.Development of pathological changes in arteries of various organs were prevented with both doses of AAS.The hypocholesterolemic effects of L-ascorbate 2-sulfate (AAS) in mice and rats have been reported in a previous paper (1) in which we suggested that AAS is a prospective hypolipidernic drug having a pronounced effect on lipid metabolism, extremely low toxicity and fewer pharmacological effects on the functions of various organs (2-4).In the present study the effects of AAS on experimental hyperlipidemia and athero sclerosis in rabbits were examined with L-ascorbate (AA) and clofibrate (CPIB) as reference compounds, and it was found that AAS possessed hypolipidemic effects in rabbits as well as in rats, and marked preventive effects on experimental atherosclerosis. MATERIALS AND METHODS Materials:The compounds examined for hypolipidemic effects are sodium L-ascorbate 2-sulfate-3 H2O, sodium L-ascorbate and clofibrate.Animal treatment: White male rabbits weighing approx. 2.5 kg were divided into 5 groups of nine and each animal was fed daily 100 g of laboratory chow (Nippon Clea, CR-1) supplemented with 1 of cholesterol. In addition to these 5 groups, one group of three

Effect of L-ascorbate 2-sulfate on fatty liver and hyperlipidemia induced by various treatments in rats and guinea pigs.

“…It was reported that AAS has hypolipidemic effects in normal rats in a dose of 250mg/kg, in cholesterol-fed rats and rabbits in doses of 150 and 300mg/kg, and in cholesterol-fed guinea pigs at a dose of 300mg/kg, respectively (4)(5)(6). In this experiments, AAS was effective at a dose of 300 mg/kg on fatty liver and/or hyperlipidemia induced by various treatments (Tables 2 and 5).…”

Fundamental Studies on Physiological and Pharmacological Actions of L-Ascorbate 2-Sulfate. Vi. Effects of L-Ascorbate 2-Sulfate on Lipid Metabolism in Guinea Pigs

“…Analyses of lipids and AA: Analysis of lipids in serum and liver were carried out as described in the previous papers (6,7). AA in various organs was extracted by homo genization with 1.4N acetic acid in 6 % metaphosphoric acid and centrifuging at 3,000 rpm for 15 min.…”

“…We reported in previous papers that AAS exerts lipid lowering effects in experimental hyperlipidemia with mice, rats and rabbits, and prevents development of atherosclerosis (6,7). In an attempt to dissociate the effect of AAS from that of L-ascorbic acid (AA), the present study of AAS on lipid metabolism has been carried out in guinea pigs which do not to biosynthesize AA in vivo.…”