Fundamentals of electrochemical detection techniques for CE and MCE

Abstract

The electroanalytical techniques of amperometry, conductometry and potentiometry match well with the instrumental simplicity of CE. Indeed, all three detection approaches have been reported for electrophoretic separations. However, the characteristics of the three methods are quite distinct and these are not related to the optical methods more commonly employed. A detailed discussion of the underlying principles of each is given. The issue of possible effects of the separation voltage on the electrochemical detection techniques is considered in depth, and approaches to the elimination of such interferences are also discussed for each case.

A review of microdialysis coupled to microchip electrophoresis for monitoring biological events

“…In general, fabrication of electrodes through classic photolithography techniques allows direct integration into microfluidic devices. Electrochemistry coupled to microchip electrophoresis has been previously reviewed [121-124]. …”

Capillary and Microchip Electrophoresis

“…7 for an example). For conductivity detection, the mobility of the buffer ion of like charge and the ionic strength of the buffer should be optimized [79], whereas volatile buffers should be employed for CE-MS [80]. Impact of buffer components and pH on separability, indirect detection, and conductivity detection is easiest assessed by simulation (see Sections 3.3 and 3.2, respectively).…”

“…In addition to ESI a range of other techniques have been applied for ionization [97]. From a detection point of view, there are other sensitive detection principles in development, including electrochemical and electrochemiluminescence approaches [39,79,98,99].…”

Determination of free and total valproic acid in human plasma by capillary electrophoresis with contactless conductivity detection

“…A further distinct advantage is the ability to carry out an analysis in very small sample volumes. Several recent general review articles on CE-C 4 D are available [26][27][28]. A series of applications of the method for clinical analysis of diverse biological samples have been reported [29][30][31][32][33][34][35][36][37][38][39][40].…”