Fundamentals of the theory and practice of polymer gel-permeation chromatography as a method of chromatographic porosimetry

Горбунов, А.А.; Solovyova, L. Ya.; Pasechnik, V.A.

doi:10.1016/s0021-9673(01)84594-5

Cited by 93 publications

(32 citation statements)

References 45 publications

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“…(7) and (8), p d represents the pore diameter, n is a dummy integration variable, and K sp denotes the kernel function [27] that represents the portioning of molecule i in a single pore. The functional form of K sp depends on the molecular structure of the polymer standards and the morphology of the pores; for straight chain polymers partitioning in cylindrical pores, the expressions developed by Casassa [41] or Davidson et al [42] should be used.…”

Section: Isecmentioning

confidence: 99%

“…Furthermore, any appropriate normalized distribution function (Gaussian distribution, log-normal distribution, Weibull distribution, etc.) can be selected to represent f b or f p ; Gorbunov et al [27] have indicated that the exclusion curve for a material is not strongly affected by the functional form of the pore size distribution.…”

Section: Isecmentioning

confidence: 99%

“…The values obtained by ISEC are listed together with those generated by nitrogen sorption measurements in Table 3. However, due to the fact that multimodal distributions within one order of magnitude are not clearly manifested in an exclusion curve [27], the real trimodal pore volume distribution indicated for the MTS2 material by nitrogen sorption was not employed in the ISEC model. Therefore, the pore volume distribution for the MTS2 material obtained from ISEC can be considered to be an effective unimodal representation of the apparently real trimodel distribution detected by nitrogen sorption porosimetry.…”

Section: Size Exclusion Propertiesmentioning

confidence: 99%

See 2 more Smart Citations

Pore structural characteristics, size exclusion properties and column performance of two mesoporous amorphous silicas and their pseudomorphically transformed MCM‐41 type derivatives

Bayram-Hahn¹,

Grimes

Lind

et al. 2007

J of Separation Science

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Highly ordered mesoporous silicas such as, mobile composition of matter, MCM-41, MCM-48, and the SBA-types of materials have helped to a large extent to understand the formation mechanisms of the pore structure of adsorbents and to improve the methods of pore structural characterization. It still remains an open question whether the high order, the regularity of the pore system, and the narrow pore size distribution of the materials will lead to a substantial benefit when these materials are employed in liquid phase separation processes. MCM-41 type 10 microm beads are synthesized following the route of pseudomorphic transformation of highly porous amorphous silicas. Highly porous silicas and the pseudomorphically transformed derivatives are characterized by nitrogen sorption at 77 K and by inverse size-exclusion chromatography (ISEC) employing polystyrene standards. Applying the network model developed by Grimes, we calculated the pore connectivity n(T) of the materials. The value of n(T) varies between the percolation threshold of the lattice and values of n(T) > 10, the latter being the limiting value above which the material can be considered to be almost infinitely connected such that the ISEC behavior of the material calculated with the pore network model is the same when calculated with a parallel pore model which assumes an infinite connectivity. One should expect that the pore connectivity is reflected in the column performance, when these native and unmodified materials are packed into columns and tested with low molecular weight analytes in the Normal Phase LC mode. As found in a previous study on monolithic silicas and highly porous silicas, the slope of the plate height (HETP) - linear velocity (u) curve decreased significantly with enhanced pore connectivity of the materials. First results on the pseudomorphically transformed MCM-41 type silicas and their highly porous amorphous precursors showed that (i) the transformation did not change the pore connectivity (within the limits detectable by ISEC) from the starting material to the final product and (ii) the slope of the HETP versus u curve for dibutylphtalate did not change significantly after the pseudomorphic transformation.

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Section: Isecmentioning

confidence: 99%

Section: Isecmentioning

confidence: 99%

Section: Size Exclusion Propertiesmentioning

confidence: 99%

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Pore structural characteristics, size exclusion properties and column performance of two mesoporous amorphous silicas and their pseudomorphically transformed MCM‐41 type derivatives

Bayram-Hahn¹,

Grimes

Lind

et al. 2007

J of Separation Science

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show abstract

“…Pressure-driven capillary liquid chromatographic inversed size-exclusion chromatography (ISEC) was performed using Bischoff 2200 (Bischoff, Leonberg, Germany) micropump, T-junction with a flow-splitting capillary, Linear  UVIS 200 (Spectra-Physics, San José, CA, USA) detector, SpectraSystem AS 3000 (Spectra-Physics) autosampler, CSW (DataApex, Prague, Czech Republic) data registration software. Porosimetric characteristics were calculated using the Kruš inskaš & Maruska 1991 software based on the theoretical ISEC model proposed by Gorbunov et al [16] …”

Section: Instrumentationmentioning

confidence: 99%

A simplified synthesis of polymeric nonparticulate stationary phases with macrocyclic antibiotic as chiral selector for capillary electrochromatography

2004

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A simplified approach to synthesize nonparticulate (continuous or monolithic) beds with embedded vancomycin chiral selectors for capillary electrochromatography is proposed. In the present approach, N,N'-diallyltartardiamide monomer with diol functionality is used, which can be readily converted to aldehyde groups via periodate treatment. Parallel to the activation of the polymeric matrix for covalent attachment of vancomycin, the periodate treatment has shown secondary effects on the polymeric bed morphology, namely the increase of the average pore size and porosity of the skeleton. Inversed size-exclusion chromatography was applied to characterize porosimetric properties of the capillary columns before and after the periodate treatment. Electroosmotic and enantioselective properties of the nonparticulate beds synthesized are presented. The approach is of more general interest attaching different affinity groups to the polymeric matrix and/or enhancing the accessibility to the active sites, for instance, in the molecular imprinting technique.

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“…Pore structural characteristics were calculated using the Krušinskas and Maruška 1991 software based on the theoretical ISEC model proposed by Gorbunov et al [30].…”

Section: Isecmentioning

confidence: 99%

Coupling of solid‐phase microextraction continuous bed (monolithic) capillaries with capillary zone electrophoresis for direct analysis of drugs in biological fluids

et al. 2008

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Hyperlink robust biocompatible solid-phase microextraction (SPME) devices were prepared using continuous bed (monolithic) restricted-access media (RAM) as the SPME capillary insert. The RAM-based SPME approach was able to simultaneously separate proteins from a biological sample, while directly extracting the active components of caffeine, paracetamol and acetylsalicylic acid from the drug NeoCitramonum. The devices were interfaced with a CZE system and fully automated analysis for sample preconcentration, desorption, separation and quantification of analytes was evaluated. Comparative study of in-line coupled SPME-CZE using RAM and RP capillary inserts was carried out. Using an SPME (RAM) insert, the calculated caffeine, paracetamol and acetylsalicylic acid LODs in a bovine plasma sample were 0.3, 0.8 and 1.9 ng/mL, respectively.

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Fundamentals of the theory and practice of polymer gel-permeation chromatography as a method of chromatographic porosimetry

Cited by 93 publications

References 45 publications

Pore structural characteristics, size exclusion properties and column performance of two mesoporous amorphous silicas and their pseudomorphically transformed MCM‐41 type derivatives

Pore structural characteristics, size exclusion properties and column performance of two mesoporous amorphous silicas and their pseudomorphically transformed MCM‐41 type derivatives

A simplified synthesis of polymeric nonparticulate stationary phases with macrocyclic antibiotic as chiral selector for capillary electrochromatography

Coupling of solid‐phase microextraction continuous bed (monolithic) capillaries with capillary zone electrophoresis for direct analysis of drugs in biological fluids

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