Fungal choline biosynthesis - a target for controlling rice blast

Uesugi, Yasuhiko

doi:10.1039/b100804h

Cited by 28 publications

(8 citation statements)

References 3 publications

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“…For instance, a plant defence activator, probenazole induces defence through systemic acquired resistance after the salicylic acid and pathogenesis-related protein accumulation is increased (Iwai et al, 2007). Other chemicals such as, melanin biosynthetic inhibitors intervene in the formation of appressorium (Iwata, 2001;Kurahashi, 2001) and choline biosynthesis fungicides target the fungal membrane phosphatidylcholine synthesis (Uesugi, 2001).…”

Section: Chemical Control Using Fungicidesmentioning

confidence: 99%

The pathogen and control management of rice blast disease

Zakaria¹,

Misman²

2018

MJM

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Outbreak of rice blast disease is a serious problem in all rice growing regions worldwide and is a threat to rice production. In Malaysia, the outbreak of rice blast is a recurrent problem where rice plant remain vulnerable to the disease. Nowadays, the generic name of rice blast pathogen is Pyricularia oryzae and Magnaporthe oryzae is the synonym. Rice pathogen infects mainly aerial parts of rice plant causing rotting and blast on the leaves, stems, peduncles, panicles and seeds leading to crop failure. Rice blast pathogen population is regarded as highly variable and consisted of multiple pathotypes which contribute to genetic diversity as well virulence diversity of the pathogen. The pathotypes can only be determined through pathogenicity test using a set of differential rice cultivars which generally differ by one or more resistance genes. Knowledge on the rice blast virulence and host resistant is important to manage the disease. Among the control methods, utilizing resistant cultivars is considered as the most effective approach to manage rice blast disease. Therefore, development of blast resistant cultivar becomes a priority.

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Section: Chemical Control Using Fungicidesmentioning

confidence: 99%

The pathogen and control management of rice blast disease

Zakaria¹,

Misman²

2018

MJM

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“…The ∆cyp684 mutants showed a 200 fold reduction in fenhexamid resistance and a simultaneous reduction in DMI-fenhexamid synergy ( In B. pseudocinerea however the cyp684 deletion abolishes the hypersensitivity to the phosphothiolate edifenphos in addition to the loss of fenhexamid resistance ( These compounds need to be modified in order to become active. In the case of edifenphos, the active metabolite is produced by the cleavage of a phosphor-sulfur bridge (Uesugi, 2001). As for B. pseudocinerea, a strong synergy was observed between phosphorothiolates and DMIs.…”

Section: Characterization Of An Unusual Resistance Mechanism Towards mentioning

confidence: 99%

Fenhexamid Resistance in the Botrytis Species Complex, Responsible for Grey Mould Disease

Billard¹,

Fillinger²,

Leroux³

et al. 2011

Fungicides - Beneficial and Harmful Aspects

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“…Because of the critical role of phospholipids in M. oryzae , key enzymes involved in phospholipid biosynthesis are used as targets of rice blast fungicides. The widely used fungicides phosphorothiolates and isoprothiolane inhibit the transmethylation step of choline biosynthesis (Uesugi, 2001). The impact of phospholipid biosynthesis on virulence has also been reported in the human pathogen Candida albicans , and phosphatidylserine (PS) and phosphatidylethanolamine (PE) are important for pathogenesis (Mu et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

The MoPah1 phosphatidate phosphatase is involved in lipid metabolism, development, and pathogenesis in Magnaporthe oryzae

Zhao

Sun

et al. 2022

Molecular Plant Pathology

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As with the majority of the hemibiotrophic fungal pathogens, the rice blast fungus Magnaporthe oryzae uses highly specialized infection structures called appressoria for plant penetration. Appressoria differentiated from germ tubes rely on enormous turgor pressure to directly penetrate the plant cell, in which process lipid metabolism plays a critical role. In this study, we characterized the MoPAH1 gene in M. oryzae, encoding a putative highly conserved phosphatidate phosphatase. The expression of MoPAH1 was up-regulated during plant infection. The MoPah1 protein is expressed at all developmental and infection stages, and is localized to the cytoplasm. Disruption of MoPAH1 causes pleiotropic defects in vegetative growth, sporulation, and heat tolerance. The lipid profile is significantly altered in the Mopah1 mutant. Lipidomics assays showed that the level of phosphatidic acid (PA) was increased in the mutant, which had reduced levels of diacylglycerol and triacylglycerol. Using a PA biosensor, we showed that the increased level of PA in the Mopah1 mutant was primarily accumulated in the vacuole. The Mopah1 mutant was blocked in both conidiation and the formation of appressorium-like structures at hyphal tips. It was nonpathogenic and failed to cause any blast lesions on rice and barley seedlings. RNA sequencing analysis revealed that MoPah1 regulates the expression of transcription factors critical for various developmental and infection-related processes. The Mopah1 mutant was reduced in the expression and phosphorylation of Pmk1 MAP kinase and delayed in autophagy. Our study demonstrates that MoPah1 is necessary for lipid metabolism, fungal development, and pathogenicity in M. oryzae.

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Fungal choline biosynthesis - a target for controlling rice blast

Cited by 28 publications

References 3 publications

The pathogen and control management of rice blast disease

The pathogen and control management of rice blast disease

Fenhexamid Resistance in the Botrytis Species Complex, Responsible for Grey Mould Disease

The MoPah1 phosphatidate phosphatase is involved in lipid metabolism, development, and pathogenesis in Magnaporthe oryzae

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