Fungal Endophyte Communities in Begonia Species from the Brazilian Atlantic Rainforest

Correia, Ana Maria Lima; Lira, Simone Possedente de; Assis, Marco Antônio; Rodrigues, André

doi:10.1007/s00284-017-1400-1

Cited by 15 publications

(15 citation statements)

References 54 publications

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“…Diaporthe was the dominant genus in both plants (Figure 3 ; Table S1 ). This genus has also been described as dominant in S. adstringens by Carvalho et al ( 2012 ) and is widely distributed in different plants of several biomes, such as the Pantanal (Hokama et al, 2017 ; Pietro-Souza et al, 2017 ), Mangrove forest (de Souza Sebastianes et al, 2013 ), Atlantic Rainforest (Correia et al, 2017 ), Cerrado and Caatinga (Ferreira et al, 2012 ; Lisboa et al, 2013 ). The ability of Diaporthe species to colonize different plants may result from the produced metabolites that bypass the plants defenses, or even by acting on host development and defense against plant pathogens (Nicoletti and Fiorentino, 2015 ; Santos et al, 2016 ; Tahir et al, 2017 ).…”

Section: Discussionmentioning

confidence: 95%

“…The community also differs among the tissues from V. divergens analyzed, not easily visible in the results of nMDS, but confirmed by ANOSIM (Figure 4 ; Tables S4 , S5 ). The main difference in the community structure comes from a higher abundance and richness of endophytes in petioles, which may be result of a specialized endophytic microbiota (Persoh, 2015 ; Correia et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

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Bioprospecting and Structure of Fungal Endophyte Communities Found in the Brazilian Biomes, Pantanal, and Cerrado

et al. 2018

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Medicinal plants have been recognized as hosts of high diverse endophytic microorganisms, including fungi that produce secondary metabolites with biological activity. Two biomes in Brazil, Pantanal (wetland), and Cerrado (savannah), are known as biodiversity hotspots, and despite their importance as a reservoir for several species, knowledge about the fungal biodiversity in these biomes is very limited. Fungal endophytic communities associated with leaves and petioles of the medicinal plants Vochysia divergens (from Pantanal) and Stryphnodendron adstringens (from Cerrado) were analyzed and studied for their antimicrobial activity against human and plant pathogens. A total of 1,146 isolates of endophytic fungi were obtained from plants collected in January and June of 2016 and grouped into 124 morphotypes. One isolate of each morphotype was identified by sequencing of internal transcribed spacer (ITS) region of the rDNA gene, which revealed the presence of 24 genera, including 3 possible new genera, and 48 taxa. Differences in the endophytic community according to the biomes were observed concerning the analyzed morphotypes. However, when we analyzed the diversity of genera and richness, they were similar for both plants, with Diaporthe, Phyllosticta, and Neofusicoccum as dominant genera. In addition, the community composition of V. divergens differs according to the analyzed plant tissues (petiole and leaf). These data suggested that both, the plant species and plant tissues play a role in the composition of endophytic community. As regards the biotechnological potential, 5 isolates showed activity against the phytopathogens Phyllosticta citricarpa, Colletotrichum abscissum, and Fusarium verticilioides, and 8 isolates showed high activity against clinical pathogens and were selected for the production of crude extract in different culture media. Extract from cultivation of Diaporthe sp. LGMF1548 and LGMF1583 and Neofusicoccum brasiliense LGMF1535 showed activity against methicillin-resistant Staphylococcus aureus, Klebssiella pneumonia, and Candida albicans. In addition, extracts of Diaporthe cf. heveae LGMF1631 inhibited 90% of the mycelial growth of the P. citricarpa and 70% of C. abscissum and may represent an alternative to be used in the biological control of these phytopathogens. Future research will focus on the chemical characterization and structural elucidation of these bioactive compounds.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Bioprospecting and Structure of Fungal Endophyte Communities Found in the Brazilian Biomes, Pantanal, and Cerrado

et al. 2018

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“…The taxonomy of PE-10, PE-17, PE-19, PE-26, and PE-38 was confirmed at the species level as Colletotrichum brevisporum [ITS BLASTn 98%; β-tubulin BLASTn 100%; 100% Bayesian probability (BP)], while PE-39 was confirmed as Curvularia senegalensis (ITS BLASTn 97%; 100% PB) ( Figure 1). Literature data had already reported the abundant occurrence of Colletotrichum in the endophytic community (Vieira, Michereff, Morais Jr, Hyde, & Câmara, 2014;Nascimento et al, 2015;Gonzaga, Costa, Santos, Araújo, & Queiroz, 2015;Correia, Lira, Assis, & Rodrigues, 2018) Herein, Curvularia endophytes were less frequent in the P. edulis community; this genus was also isolated as an endophytes from other vegetal species (Bezerra et al, 2013;Nascimento et al, 2015;Correia et al, 2018).…”

Section: Isolation and Molecular Identification Of Endophytic Fungimentioning

confidence: 99%

Bioprospection and molecular phylogeny of culturable endophytic fungi associated with yellow passion fruit

Oliveira

Polli

Polônio

et al. 2020

Acta Sci. Biol. Sci.

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Endophytic microorganisms live inside the plants without causing any damage to their hosts. In the agricultural field, these endophytes might be a strategy of biological control for phytopathogens. We aimed to isolate endophytic fungi from yellow passion fruit (Passiflora edulis) leaves, evaluating its biocontrol capacity by in vitro antagonism against phytopathogen Colletotrichum sp. CNPU378. We also carried out greenhouse experiments in bean seedlings. A high colonization frequency was obtained (89%), and the molecular identification based on DNA sequencing attested Colletotrichum as the most frequent genus and minor occurrence of Curvularia endophytes. The endophytes tested showed different types of competitive interactions in in vitro antagonism inhibition rate ranging from 28.8 to 48.8%. There were 10 promising antagonists tested for their antagonist activity of crude extracts of secondary metabolites, in which strain PE-36 (20.8%) stood out among the other strains evaluated. In the greenhouse assay, plants inoculated only with endophyte Colletotrichum sp. PE-36 was symptomless and suggest that the endophyte strengthened the growth promotion in common bean plants, especially in the root length and number of leaves when compared to control plants and other treatments. Despite many fungi of Colletotrichum genus being described as causative agents of anthracnose, in this study, the plant sampled was colonized predominantly by Colletotrichum endophytes living in asymptomatic relationship. By the way, we come across a Colletotrichum sp. endophyte able to antagonize a Colletotrichum sp. pathogen.

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“…& B.G. Schub (HRCB 64229) and identified by morphological and molecular techniques in a previous study (Correia, Lira, Assis, & Rodrigues, 2018).…”

Section: Biological Materialsmentioning

confidence: 99%

“…Here, we worked with several endophytic fungi from plants in the genus Begonia Plumier found in the Atlantic Rain Forest (Correia, Lira, Assis, & Rodrigues, 2018), to assess their potential to produce active extracts against plant pathogens and identify the compounds.…”

Section: Introductionmentioning

confidence: 99%

Secondary metabolites produced by endophytic fungi: novel antifungal activity of fumiquinone B

Grigoletto¹,

Correia²,

Abraham³

et al. 2019

Acta Sci. Biol. Sci.

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Fungi are present in the most diverse environments including the interior of plant tissues, living as endophytes without causing apparent damage. These endophytes are producers of secondary metabolites, also known as natural products, such as fungicides. Here, we evaluated the ethyl acetate fractions obtained from endophytic fungi isolated from plants in the genus Begonia. The fractions were submitted to inhibitory test against the plant pathogens Diaporthe phaseolorum and Colletotrichum gloeosporioides. From the 88 ethyl acetate fractions evaluated, 14.7 % inhibited C. gloeosporioides and 11.3 % inhibited D. phaseolorum. One fungal isolate displaying an active fraction was selected for chemical investigation. The fungus identified as Neopestalotiopsis sp., produced a compound that was active against D. phaseolorum, with a MIC of 312 µg mL-1 (1,695.3 µM). The compound was identified by mass spectrometry and 1H NMR as the known compound fumiquinone B. The results highlight that the endophytes are capable of producing compounds that may be used to control plant pathogens. The compound fumiquinone B is reported for the first time as an antifungal agent against D. phaseolorum, a relevant plant pathogen worldwide. This is also the first report of the production of fumiquinone B by the genus Neopestalotiopsis.

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Fungal Endophyte Communities in Begonia Species from the Brazilian Atlantic Rainforest

Cited by 15 publications

References 54 publications

Bioprospecting and Structure of Fungal Endophyte Communities Found in the Brazilian Biomes, Pantanal, and Cerrado

Bioprospecting and Structure of Fungal Endophyte Communities Found in the Brazilian Biomes, Pantanal, and Cerrado

Bioprospection and molecular phylogeny of culturable endophytic fungi associated with yellow passion fruit

Secondary metabolites produced by endophytic fungi: novel antifungal activity of fumiquinone B

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