Fungal food choices of<i>Dermatophagoides farinae</i>affect indoor fungi selection and dispersal

Naegele, A.; Reboux, Gabriel; Scherer, Émeline; Roussel, Sandrine; Millon, Laurence

doi:10.1080/09603123.2012.699029

Cited by 25 publications

(8 citation statements)

References 15 publications

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“…Most research groups use dried yeast to supplement diets and improve mite population growth [72, 80–83]. HDM have been observed to feed on bacteria and fungi in laboratory experiments [84, 85]. Whether HDM consume bacteria or fungi in a wildtype setting as a nutrient source needs further experimental evidence.…”

Section: Resultsmentioning

confidence: 99%

Proteome and allergenome of the European house dust mite Dermatophagoides pteronyssinus

et al. 2019

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The European house dust mite Dermatophagoides pteronyssinus is of significant medical importance as it is a major elicitor of allergic illnesses. In this analysis we have undertaken comprehensive bioinformatic and proteomic examination of Dermatophagoides pteronyssinus airmid, identified 12,530 predicted proteins and validated the expression of 4,002 proteins. Examination of homology between predicted proteins and allergens from other species revealed as much as 2.6% of the D . pteronyssinus airmid proteins may cause an allergenic response. Many of the potential allergens have evidence for expression ( n = 259) and excretion ( n = 161) making them interesting targets for future allergen studies. Comparative proteomic analysis of mite body and spent growth medium facilitated qualitative assessment of mite group allergen localisation. Protein extracts from house dust contain a substantial number of uncharacterised D . pteronyssinus proteins in addition to known and putative allergens. Novel D . pteronyssinus proteins were identified to be highly abundant both in house dust and laboratory cultures and included numerous carbohydrate active enzymes that may be involved in cuticle remodelling, bacteriophagy or mycophagy. These data may have clinical applications in the development of allergen-specific immunotherapy that mimic natural exposure. Using a phylogenomic approach utilising a supermatrix and supertree methodologies we also show that D . pteronyssinus is more closely related to Euroglyphus maynei than Dermatophagoides farinae .

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Section: Resultsmentioning

confidence: 99%

Proteome and allergenome of the European house dust mite Dermatophagoides pteronyssinus

et al. 2019

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“…Mites are associated with microorganisms, including bacteria and fungi. The bacteria and fungi contribute to mite nutrition by producing exo-enzymes that predigest the substrate by serving directly as a food source (Sinha, 1966 ; Hubert et al, 2003 , 2012a , b ; Smrz, 2003 ; Erban and Hubert, 2008 ; Naegele et al, 2013 ). From a sanitary view, the mite feces are the most significant contaminants of the human environment because they contain major allergens that accumulate and persist in the environment (Tovey et al, 1981 ; de Boer et al, 1995 ; Sidenius et al, 2002 ; Platts-Mills and Woodfolk, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Feces Derived Allergens of Tyrophagus putrescentiae Reared on Dried Dog Food and Evidence of the Strong Nutritional Interaction between the Mite and Bacillus cereus Producing Protease Bacillolysins and Exo-chitinases

et al. 2016

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Tyrophagus putrescentiae (Schrank, 1781) is an emerging source of allergens in stored products and homes. Feces proteases are the major allergens of astigmatid mites (Acari: Acaridida). In addition, the mites are carriers of microorganisms and microbial adjuvant compounds that stimulate innate signaling pathways. We sought to analyze the mite feces proteome, proteolytic activities, and mite-bacterial interaction in dry dog food (DDF). Proteomic methods comprising enzymatic and zymographic analysis of proteases and 2D-E-MS/MS were performed. The highest protease activity was assigned to trypsin-like proteases; lower activity was assigned to chymotrypsin-like proteases, and the cysteine protease cathepsin B-like had very low activity. The 2D-E-MS/MS proteomic analysis identified mite trypsin allergen Tyr p3, fatty acid-binding protein Tyr p13 and putative mite allergens ferritin (Grp 30) and (poly)ubiquitins. Tyr p3 was detected at different positions of the 2D-E. It indicates presence of zymogen at basic pI, and mature-enzyme form and enzyme fragment at acidic pI. Bacillolysins (neutral and alkaline proteases) of Bacillus cereus symbiont can contribute to the protease activity of the mite extract. The bacterial exo-chitinases likely contribute to degradation of mite exuviae, mite bodies or food boluses consisting of chitin, including the peritrophic membrane. Thus, the chitinases disrupt the feces and facilitate release of the allergens. B. cereus was isolated and identified based on amplification and sequencing of 16S rRNA and motB genes. B. cereus was added into high-fat, high-protein (DDF) and low-fat, low-protein (flour) diets to 1 and 5% (w/w), and the diets palatability was evaluated in 21-day population growth test. The supplementation of diet with B. cereus significantly suppressed population growth and the suppressive effect was higher in the high-fat, high-protein diet than in the low-fat, low-protein food. Thus, B. cereus has to coexist with the mite in balance to be beneficial for the mite. The mite-B. cereus symbiosis can be beneficial-suppressive at some level. The results increase the veterinary and medical importance of the allergens detected in feces. The B. cereus enzymes/toxins are important components of mite allergens. The strong symbiotic association of T. putrescentiae with B. cereus in DDF was indicated.

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“…Su et al (2005) showed that the level of total IgE was significantly higher in children exposed to both molds and dust mites. However, dust mites are also involved in both mold spore consumption and dispersion (Naegele et al 2013). Therefore, the interactions between mold and dust mites and the development of asthma in Puerto Rico will need further exploration.…”

Section: Discussionmentioning

confidence: 99%

Mold populations and dust mite allergen concentrations in house dust samples from across Puerto Rico

Vesper¹,

Choi²,

Perzanowski³

et al. 2015

International Journal of Environmental Health Research

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Lifetime childhood asthma prevalence (LCAP) percentages in Puerto Rico Health Regions (HR) are substantially higher in northeastern vs. southwestern HR. Higher average relative humidity in the northeast might promote mold and mite exposures and possibly asthma prevalence. To test this hypothesis, mold contamination, Environmental Relative Moldiness Index (ERMI) values were measured in floor dust (n = 26) and dust mite allergen concentrations in bed dust (n = 14). For this analysis, the eight HR were divided into those with LCAP > 30 % (n = 3) and < 30 % (n = 5). The average ERMI value was significantly greater (Wilcoxon Rank Sum, p < 0.001) in high than in low LCAP HR (14.5 vs. 9.3). The dust mite antigens Der p 1, Der f 1, and Blo t 5 were detected in 90 % of bed samples, but the concentrations were not significantly different in high vs. low LCAP HR. Mold exposures might partially explain the differences in LCAP HR in Puerto Rico.

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Fungal food choices ofDermatophagoides farinaeaffect indoor fungi selection and dispersal

Cited by 25 publications

References 15 publications

Proteome and allergenome of the European house dust mite Dermatophagoides pteronyssinus

Proteome and allergenome of the European house dust mite Dermatophagoides pteronyssinus

Feces Derived Allergens of Tyrophagus putrescentiae Reared on Dried Dog Food and Evidence of the Strong Nutritional Interaction between the Mite and Bacillus cereus Producing Protease Bacillolysins and Exo-chitinases

Mold populations and dust mite allergen concentrations in house dust samples from across Puerto Rico

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