Fungal morphology and fragmentation behavior in a fed-batchAspergillus oryzae fermentation at the production scale

Li, Zheng Jian; Shukla, Vivek Kumar; Fordyce, Andrew P.; Pedersen, Annemarie Gade; Wenger, Kevin S.; Marten, Mark R.

doi:10.1002/1097-0290(20001105)70:3<300::aid-bit7>3.0.co;2-3

Cited by 85 publications

(47 citation statements)

References 51 publications

(93 reference statements)

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“…Good mixing, mass and heat transfer require a threshold level of agitation. On the other hand, a high agitation rate leads to a high energy dissipation rate connected with high shear stress, which may result in fragmentation and damage of cells and the mycelial network [22][23][24][25] . The optimal growth pH for Polyporus sp.…”

Section: Resultsmentioning

confidence: 99%

Effect of Environmental Factors in the Decolorization of Remazol Brilliant Blue R by Polyporus Sp. S133

Hadibarata

Kristanti

2012

J. Chil. Chem. Soc.

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The effects of environmental conditions such as pH, agitation, carbon and nitrogen sources, metal ion, salinity and phenolic compound on the decolorization of the anthraquinone type textile dyestuff Remazol Brilliant Blue R by white rot fungi, Polyporus sp. S133 were investigated. After extensive testing, the best performance took place at pH 4 and decolorization of the dye in liquid effluents was significantly increased by agitation. Compared to other carbon and nitrogen sources tested, glucose and ammonium tartrate gave rise to better decolorization performances. Decolorization of RBBR occurred in the presence of metal ions which are typically found in textile industry effluents. Of all the metal ions tested, Fe ++ was the most inhibiting of the decolorization. The effect of culture salinity on decolorization was also investigated. Under high-salt conditions, RBBR was also decolorized completely in 6 d. The presence of phenolic compounds inhibited the decolorization at a concentration of 1 mM, but protocatechuic acid showed no inhibition. The results indicate that possibly anthraquinone type dyes such as RBBR act as enzyme substrates that are directly oxidized by laccase.

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Section: Resultsmentioning

confidence: 99%

Effect of Environmental Factors in the Decolorization of Remazol Brilliant Blue R by Polyporus Sp. S133

Hadibarata

Kristanti

2012

J. Chil. Chem. Soc.

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“…Power, agitator speed and airflow rate were reported as energy dissipation/circulation function (EDCF) and aeration number (N Ae ) as described here. EDCF (in watts per cubic metre second), was found to correlate better with morphological and fragmentation data than power per unit liquid volume (P g /V L ) Amanullah et al 1999;Justen et al 1998;Li et al 2000). In a high-viscosity system, the gasfilled cavities behind the impeller blades were quite stable, and their size was almost independent of gassing rate (Nienow 1990).…”

Section: Energy Dissipation Per Circulation Function and Aeration Nummentioning

confidence: 99%

Impact of aeration and agitation on metabolic heat and protease secretion of Aspergillus tamarii in a real-time biological reaction calorimeter

Dhandapani

Surianarayanan

Dhilipkumar

et al. 2012

Appl Microbiol Biotechnol

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The effects of aeration and agitation on metabolic heat, alkaline protease production and morphology for Aspergillus tamarii MTCC5152 are reported in this manuscript. Measurement of metabolic heat has been attempted by the continuous and dynamic heat balance method in a biological real-time reaction calorimeter. At lower agitation intensities, growth-related processes were dominating. As a result the protease activity and the product heat yields were lower than those for 350 and 450 rpm. Although biomass growth was necessary to obtain maximum protease yield, agitation seemed to play a vital role in the protease production process. Energy dissipation per circulation function of the process is also deduced from power input. At optimal conditions, 350 rpm and 1 vvm, the gassed power required was 0.133 W. Pellet morphology and protease production were studied under different aeration and agitation intensities of A. tamarii. Pellet structure was considerably influenced by DO, a higher DO level resulted in denser pellets (1,018.4 kg/m(3)) leading to higher protease activity. Coupling of hydrodynamics and bio-reaction highlighted the complex relationship between energy dissipation, substrate uptake rate and fungal physiology. This study emphasised the potential of biocalorimetry as a reliable monitoring and robust control tool for aerobic fermentation of A. tamarii, using agricultural by-products.

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“…All flasks were incubated at 307C on an oscillating shaker at 250 rpm. Samples were taken at different time intervals and mycelia were harvested by filtration with a Büchner funnel and their wet/dry weights (biomass) were measured [17].…”

Section: Strains and Growth Conditionsmentioning

confidence: 99%

Comparison of lysis methods and preparation protocols for one- and two-dimensional electrophoresis of Aspergillus oryzae intracellular proteins

Nandakumar

Marten

2002

Electrophoresis

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Filamentous fungal fermentations are used to produce billions of dollars of biochemical and pharmaceutical products annually, yet are plagued by a number of poorly understood problems that would benefit from proteomic analysis. Unfortunately, few publications are available which describe extraction of filamentous fungal proteins for two-dimensional electrophoresis. The goal here was to develop protocols for extraction of fungal proteins, from both wild-type and a recombinant strain of the industrially important filamentous fungi Aspergillus oryzae, to be used for both one- and two-dimensional electrophoresis (1-DE and 2-DE). Because fungal cell walls are exceptionally resistant to fragmentation, four lysis protocols were tested: (i) boiling in strong alkali solution, (ii) boiling in Sodium dodecyl surfate (SDS), (iii) chemical lysis in Y-PER(R) reagent, and (iv) mechanical lysis via rapid agitation with glass beads in a Mini-BeadBeater(R). For both 1-DE and 2-DE, rapid agitation with glass beads was found to be the most efficient extraction method, yielding both mini- and large-format gels with little streaking or spot tailing, and proteins comprising a broad range of molecular weights and pI values.

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Fungal morphology and fragmentation behavior in a fed-batchAspergillus oryzae fermentation at the production scale

Cited by 85 publications

References 51 publications

Effect of Environmental Factors in the Decolorization of Remazol Brilliant Blue R by Polyporus Sp. S133

Effect of Environmental Factors in the Decolorization of Remazol Brilliant Blue R by Polyporus Sp. S133

Impact of aeration and agitation on metabolic heat and protease secretion of Aspergillus tamarii in a real-time biological reaction calorimeter

Comparison of lysis methods and preparation protocols for one- and two-dimensional electrophoresis of Aspergillus oryzae intracellular proteins

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