Fungi of the Genera Alternaria as Producers of Biological Active Compounds and Mycoherbicides

Dalinova, Anna; Salimova, D. R.; Berestetskiy, Alexander

doi:10.1134/s0003683820030023

Cited by 26 publications

(24 citation statements)

References 166 publications

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“…altersetin), cytotoxic activity (bi -and tricycloalternarenes) and other interesting properties (enzyme inhibitors, antioxidants, etc.). Many of them possesses several types of biological activity [33][34][35]. In the present and earlier studies [21], the extracts from A. tenuissima MFP253011 cultures obtained by various methods (using different media and solvents) were found to display entomotoxic activity along with phytotoxic, antimicrobial and cytotoxic properties (Figures 2-5).…”

Section: Detection Of Entomotoxic Metabolites In a Tenuissima Extractsmentioning

confidence: 59%

Entomotoxic Activity of the Extracts from the Fungus, <em>Alternaria tenuissima</em> and Its Major Metabolite, Tenuazonic Acid

Salimova¹,

Dalinova²,

Dubovik³

et al. 2021

Preprint

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Study of fungal antibiotics in their competitive interactions with arthropods may lead to development novel biorational insecticides. Extracts of Alternaria tenuissima MFP253011 obtained by various methods showed a wide range of biological activity, including entomotoxic properties. Analysis of their composition and bioactivity allowed to reveal several known mycotoxins and unidentified compounds that may be involved in entomotoxic activity of the extracts. Among them, tenuazonic acid (TeA), which was the major component of the A. tenuissima extracts, was found the most likely to have larvicidal activity against Galleria mellonella. In the intrahaemocoel injection bioassay, TeA was toxic to G. mellonella and of Zophobas morio with LT50 6 and 2 days, respectively, at the level of 50 µg/larva. Administered orally, TeA inhibited growth of G. mellonella larvae and caused mortality of Acheta domesticus imagines (LT50 7 days) at a concentration of 250 µg/g of feed. TeA showed weak contact-intestinal activity against the two phytophages, Tetranychus urticae and Schizaphis graminum, causing the 12 and 40% of mortality at a concentration of 1 mg/mL. TeA was cytotoxic to Sf9 cell line (IC50 25 µg/mL). Thus, model insect G. mellonella and cell line Sf9 could be used for a further toxicological characterization of TeA.

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Section: Detection Of Entomotoxic Metabolites In a Tenuissima Extractsmentioning

confidence: 59%

Entomotoxic Activity of the Extracts from the Fungus, <em>Alternaria tenuissima</em> and Its Major Metabolite, Tenuazonic Acid

Salimova¹,

Dalinova²,

Dubovik³

et al. 2021

Preprint

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“…On the other hand, a significantly higher frequency of Alternaria (Alternaria alternata) and Cladosporium (Cladosporium chasmanthicola) was observed in disordered foragers. Alternaria alternata was one of the most common phytopathogens capable of producing phytotoxins, mycotoxins and other secondary toxic metabolites [68,69]. Cladosporium was commonly found in pollen [70] and existed in a beehive environment [71].…”

Section: Discussionmentioning

confidence: 99%

Microbiota dysbiosis in honeybee ( Apis mellifera L . ) larvae infected with brood diseases and foraging bees exposed to agrochemicals

Fan

et al. 2021

R. Soc. open sci.

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American foulbrood (AFB) disease and chalkbrood disease (CBD) are important bacterial and fungal diseases, respectively, that affect honeybee broods. Exposure to agrochemicals is an abiotic stressor that potentially weakens honeybee colonies. Gut microflora alterations in adult honeybees associated with these biotic and abiotic factors have been investigated. However, microbial compositions in AFB- and CBD-infected larvae and the profile of whole-body microbiota in foraging bees exposed to agrochemicals have not been fully studied. In this study, bacterial and fungal communities in healthy and diseased (AFB/CBD) honeybee larvae were characterized by amplicon sequencing of bacterial 16S rRNA gene and fungal internal transcribed spacer1 region, respectively. The bacterial and fungal communities in disordered foraging bees poisoned by agrochemicals were analysed. Our results revealed that healthy larvae were significantly enriched in bacterial genera Lactobacillus and Stenotrophomonas and the fungal genera Alternaria and Aspergillus . The enrichment of these microorganisms, which had antagonistic activities against the etiologic agents for AFB and CBD, respectively, may protect larvae from potential infection. In disordered foraging bees, the relative abundance of bacterial genus Gilliamella and fungal species Cystofilobasidium macerans were significantly reduced, which may compromise hosts' capacities in nutrient absorption and immune defence against pathogens. Significantly higher frequency of environmentally derived fungi was observed in disordered foraging bees, which reflected the perturbed microbiota communities of hosts. Results from PICRUSt and FUNGuild analyses revealed significant differences in gene clusters of bacterial communities and fungal function profiles. Overall, results of this study provide references for the composition and function of microbial communities in AFB- and CBD-infected honeybee larvae and foraging bees exposed to agrochemicals.

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“…Metabolites of small-spored Alternaria species have been characterized as mycotoxins (tenuazonic acid, alternariol and its methyl ester, altertoxins) and as substances with phytotoxic (e.g., altenuene, tenuazonic acid, tentoxin, and a number of host-specific toxins), antimicrobial (e.g., altersetin), cytotoxic activity (bi- and tricycloalternarenes), and other interesting properties (enzyme inhibitors, antioxidants, etc.). Many of them possess several types of biological activity [ 33 , 34 , 35 ]. In the present and earlier studies [ 21 ], the extracts from A. tenuissima MFP253011 cultures obtained by various methods (using different media and solvents) were found to display entomotoxic activity along with phytotoxic, antimicrobial, and cytotoxic properties ( Figure 2 , Figure 3 , Figure 4 and Figure 5 ).…”

Section: Discussionmentioning

confidence: 99%

Entomotoxic Activity of the Extracts from the Fungus, Alternaria tenuissima and Its Major Metabolite, Tenuazonic Acid

Salimova

Dalinova

Dubovik

et al. 2021

JoF

View full text Add to dashboard Cite

The study of fungal antibiotics in their competitive interactions with arthropods may lead to the development of novel biorational insecticides. Extracts of Alternaria tenuissima MFP253011 obtained using various methods showed a wide range of biological activities, including entomotoxic properties. Analysis of their composition and bioactivity allowed us to reveal several known mycotoxins and unidentified compounds that may be involved in the entomotoxic activity of the extracts. Among them, tenuazonic acid (TeA), which was the major component of the A. tenuissima extracts, was found the most likely to have larvicidal activity against Galleria mellonella. In the intrahaemocoel injection bioassay, TeA was toxic to G. mellonella and of Zophobas morio with an LT50 of 6 and 2 days, respectively, at the level of 50 µg/larva. Administered orally, TeA inhibited the growth of G. mellonella larvae and caused mortality of Acheta domesticus adults (LT50 7 days) at a concentration of 250 µg/g of feed. TeA showed weak contact intestinal activity against the two phytophages, Tetranychus urticae and Schizaphis graminum, causing 15% and 27% mortality at a concentration of 1 mg/mL, respectively. TeA was cytotoxic to the Sf9 cell line (IC50 25 µg/mL). Thus, model insects such as G. mellonella could be used for further toxicological characterization of TeA.

show abstract

Fungi of the Genera Alternaria as Producers of Biological Active Compounds and Mycoherbicides

Cited by 26 publications

References 166 publications

Entomotoxic Activity of the Extracts from the Fungus, <em>Alternaria tenuissima</em> and Its Major Metabolite, Tenuazonic Acid

Entomotoxic Activity of the Extracts from the Fungus, <em>Alternaria tenuissima</em> and Its Major Metabolite, Tenuazonic Acid

Microbiota dysbiosis in honeybee ( Apis mellifera L . ) larvae infected with brood diseases and foraging bees exposed to agrochemicals

Entomotoxic Activity of the Extracts from the Fungus, Alternaria tenuissima and Its Major Metabolite, Tenuazonic Acid

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